Construct: ORF ccsbBroad304_02972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014985.1_s304c1, BRDN0000405935
- Derived from:
- ccsbBroadEn_02972
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAGE2E (26749)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02972
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 729408 | GAGE2D | G antigen 2D | NM_001098407.1 | 100% | 100% | |
| 2 | human | 26749 | GAGE2E | G antigen 2E | NM_001127200.3 | 100% | 100% | |
| 3 | human | 100101629 | GAGE8 | G antigen 8 | NM_012196.1 | 100% | 100% | |
| 4 | human | 645037 | GAGE2B | G antigen 2B | NM_001098411.3 | 99.7% | 99.1% | 220C>G |
| 5 | human | 2574 | GAGE2C | G antigen 2C | NM_001472.2 | 99.7% | 99.1% | 220C>G |
| 6 | human | 729447 | GAGE2A | G antigen 2A | NM_001127212.1 | 99.4% | 98.2% | 175C>G;220C>G |
| 7 | human | 645051 | GAGE13 | G antigen 13 | NM_001098412.2 | 98.8% | 98.2% | 25_27delTAT;31T>C |
| 8 | human | 2576 | GAGE4 | G antigen 4 | NM_001474.1 | 98% | 97.4% | (many diffs) |
| 9 | human | 729396 | GAGE12J | G antigen 12J | NM_001098406.2 | 98% | 96.5% | (many diffs) |
| 10 | human | 100008586 | GAGE12F | G antigen 12F | NM_001098405.2 | 97.7% | 96.5% | (many diffs) |
| 11 | human | 645073 | GAGE12G | G antigen 12G | NM_001098409.2 | 97.7% | 96.5% | (many diffs) |
| 12 | human | 729428 | GAGE12B | G antigen 12B | NM_001127345.2 | 97.7% | 96.5% | (many diffs) |
| 13 | human | 2577 | GAGE5 | G antigen 5 | NM_001475.1 | 97.7% | 96.5% | (many diffs) |
| 14 | human | 26748 | GAGE12I | G antigen 12I | NM_001477.1 | 97.7% | 96.5% | (many diffs) |
| 15 | human | 2579 | GAGE7 | G antigen 7 | NM_021123.3 | 97.7% | 96.5% | (many diffs) |
| 16 | human | 2543 | GAGE1 | G antigen 1 | NM_001040663.2 | 97.4% | 96.5% | (many diffs) |
| 17 | human | 729422 | GAGE12C | G antigen 12C | NM_001098408.1 | 97.4% | 95.7% | (many diffs) |
| 18 | human | 729431 | GAGE12E | G antigen 12E | NM_001098418.1 | 97.4% | 95.7% | (many diffs) |
| 19 | human | 100132399 | GAGE12D | G antigen 12D | NM_001127199.1 | 97.4% | 95.7% | (many diffs) |
| 20 | human | 2578 | GAGE6 | G antigen 6 | NM_001476.1 | 97.4% | 95.7% | (many diffs) |
| 21 | human | 102724473 | GAGE10 | G antigen 10 | NM_001098413.3 | 97.4% | 93.9% | (many diffs) |
| 22 | human | 729442 | GAGE12H | G antigen 12H | NM_001098410.1 | 97.1% | 94.8% | (many diffs) |
| 23 | human | 2543 | GAGE1 | G antigen 1 | XM_011543896.2 | 88.4% | 87.9% | (many diffs) |
| 24 | human | 102724473 | GAGE10 | G antigen 10 | XM_024452325.1 | 77.9% | 73.2% | (many diffs) |
| 25 | human | 645073 | GAGE12G | G antigen 12G | NM_001354422.1 | 62.1% | 61.5% | (many diffs) |
| 26 | human | 2543 | GAGE1 | G antigen 1 | NR_102272.1 | 10.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 414
- ORF length:
- 348
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ttggcgagga agatcgacct atcggcctag accaagacgc tacgtagagc 121 ctcctgaaat gattgggcct atgcggcccg agcagttcag tgatgaagtg gaaccagcaa 181 cacctgaaga aggggaacca gcaactcaac gtcaggatcc tgcagctgct caggagggag 241 aggatgaggg agcatctgca ggtcaagggc cgaagcctga agctgatagc caggaacagg 301 gtcacccaca gactgggtgt gagtgtgaag atggtcctga tgggcaggag atggacccgc 361 caaatccaga ggaggtgaaa acgcctgaag aaggtgaaaa gcaatcacag tgttacccca 421 actttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct 481 acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt 541 a