Transcript: Human NM_001098413.3

Homo sapiens G antigen 10 (GAGE10), mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
GAGE10 (102724473)
Length:
561
CDS:
117..467

Additional Resources:

NCBI RefSeq record:
NM_001098413.3
NBCI Gene record:
GAGE10 (102724473)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001098413.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000255387 AGGAACAGGTTCACCCAAAGA pLKO_005 343 CDS 100% 4.950 2.970 N GAGE10 n/a
2 TRCN0000255384 CCCTGAAATGATTGGGCCTAT pLKO_005 173 CDS 100% 4.050 2.430 N GAGE10 n/a
3 TRCN0000197332 CCTGAAATGATTGGGCCTATG pLKO.1 174 CDS 100% 6.000 3.000 Y GAGE4 n/a
4 TRCN0000137608 CCAAATCCAGAGGAGGTGAAA pLKO.1 411 CDS 100% 4.950 2.475 Y GAGE1 n/a
5 TRCN0000155993 CCAAATCCAGAGGAGGTGAAA pLKO.1 411 CDS 100% 4.950 2.475 Y GAGE2C n/a
6 TRCN0000115784 CCCGAGCAGTTCAGTGATGAA pLKO.1 198 CDS 100% 4.950 2.475 Y GAGE7 n/a
7 TRCN0000369735 CCGAGCAGTTCAGTGATGAAG pLKO_005 199 CDS 100% 4.950 2.475 Y GAGE4 n/a
8 TRCN0000138397 CGAGCAGTTCAGTGATGAAGT pLKO.1 200 CDS 100% 4.950 2.475 Y GAGE1 n/a
9 TRCN0000137684 GAACCAGCAACACCTGAAGAA pLKO.1 222 CDS 100% 4.950 2.475 Y GAGE1 n/a
10 TRCN0000155031 GAACCAGCAACACCTGAAGAA pLKO.1 222 CDS 100% 4.950 2.475 Y GAGE2C n/a
11 TRCN0000369730 GGAACCAGCAACACCTGAAGA pLKO_005 221 CDS 100% 4.950 2.475 Y GAGE4 n/a
12 TRCN0000371197 ACCAGCAACACCTGAAGAAGG pLKO_005 224 CDS 100% 4.050 2.025 Y GAGE2D n/a
13 TRCN0000263270 AGACCAAGACGCTACGTAGAG pLKO_005 150 CDS 100% 4.050 2.025 Y GAGE13 n/a
14 TRCN0000256037 AGTTCAGTGATGAAGTGGAAC pLKO_005 205 CDS 100% 4.050 2.025 Y GAGE2D n/a
15 TRCN0000370840 ATGAAGTGGAACCAGCAACAC pLKO_005 214 CDS 100% 4.050 2.025 Y GAGE6 n/a
16 TRCN0000255388 CAGCAACTCAACGTCAGGATC pLKO_005 250 CDS 100% 4.050 2.025 Y GAGE10 n/a
17 TRCN0000243153 CTAGACCAAGACGCTACGTAG pLKO_005 148 CDS 100% 4.050 2.025 Y GAGE2E n/a
18 TRCN0000243156 CTGAAATGATTGGGCCTATGC pLKO_005 175 CDS 100% 4.050 2.025 Y GAGE2E n/a
19 TRCN0000243154 CTGAAGCTGATAGCCAGGAAC pLKO_005 328 CDS 100% 4.050 2.025 Y GAGE2E n/a
20 TRCN0000262876 GAAGCTGATAGCCAGGAACAG pLKO_005 330 CDS 100% 4.050 2.025 Y GAGE12J n/a
21 TRCN0000265695 GAGCAGTTCAGTGATGAAGTG pLKO_005 201 CDS 100% 4.050 2.025 Y GAGE12F n/a
22 TRCN0000155220 GATGAAGTGGAACCAGCAACA pLKO.1 213 CDS 100% 4.050 2.025 Y GAGE2C n/a
23 TRCN0000162881 GATGAAGTGGAACCAGCAACA pLKO.1 213 CDS 100% 4.050 2.025 Y GAGE12I n/a
24 TRCN0000243315 GCCAAATCCAGAGGAGGTGAA pLKO_005 410 CDS 100% 4.050 2.025 Y GAGE12I n/a
25 TRCN0000255386 GTGGAACCAGCAACACCTGAA pLKO_005 219 CDS 100% 4.050 2.025 Y GAGE10 n/a
26 TRCN0000282435 TAGACCAAGACGCTACGTAGA pLKO_005 149 CDS 100% 4.050 2.025 Y GAGE2B n/a
27 TRCN0000281477 TGAAGCTGATAGCCAGGAACA pLKO_005 329 CDS 100% 4.050 2.025 Y GAGE2D n/a
28 TRCN0000242734 TGATGAAGTGGAACCAGCAAC pLKO_005 212 CDS 100% 4.050 2.025 Y GAGE5 n/a
29 TRCN0000255718 TGGAACCAGCAACACCTGAAG pLKO_005 220 CDS 100% 4.050 2.025 Y GAGE12F n/a
30 TRCN0000242732 GCCGAAGCCTGAAGCTGATAG pLKO_005 320 CDS 100% 3.600 1.800 Y GAGE5 n/a
31 TRCN0000371199 CCTAGACCAAGACGCTACGTA pLKO_005 147 CDS 100% 3.000 1.500 Y GAGE2D n/a
32 TRCN0000244666 ACCAGCAACTCAACGTCAGGA pLKO_005 248 CDS 100% 2.640 1.320 Y GAGE4 n/a
33 TRCN0000265767 AGCAGTTCAGTGATGAAGTGG pLKO_005 202 CDS 100% 2.640 1.320 Y GAGE12H n/a
34 TRCN0000265850 AGTGGAACCAGCAACACCTGA pLKO_005 218 CDS 100% 2.640 1.320 Y GAGE12E n/a
35 TRCN0000282344 CAACTCAACGTCAGGATCCTG pLKO_005 253 CDS 100% 2.640 1.320 Y GAGE12G n/a
36 TRCN0000121911 CAGTTCAGTGATGAAGTGGAA pLKO.1 204 CDS 100% 2.640 1.320 Y GAGE7 n/a
37 TRCN0000136873 CAGTTCAGTGATGAAGTGGAA pLKO.1 204 CDS 100% 2.640 1.320 Y GAGE1 n/a
38 TRCN0000115786 CCAGCAACTCAACGTCAGGAT pLKO.1 249 CDS 100% 2.640 1.320 Y GAGE7 n/a
39 TRCN0000263272 CCTGAAGCTGATAGCCAGGAA pLKO_005 327 CDS 100% 2.640 1.320 Y GAGE13 n/a
40 TRCN0000262618 GAAGTGGAACCAGCAACACCT pLKO_005 216 CDS 100% 2.640 1.320 Y GAGE12G n/a
41 TRCN0000281478 GCAACTCAACGTCAGGATCCT pLKO_005 252 CDS 100% 2.640 1.320 Y GAGE2D n/a
42 TRCN0000155329 GCAGTTCAGTGATGAAGTGGA pLKO.1 203 CDS 100% 2.640 1.320 Y GAGE2C n/a
43 TRCN0000250096 GGAACCAGCAACTCAACGTCA pLKO_005 245 CDS 100% 2.640 1.320 Y GAGE6 n/a
44 TRCN0000163548 GTGATGAAGTGGAACCAGCAA pLKO.1 211 CDS 100% 2.640 1.320 Y GAGE12I n/a
45 TRCN0000265783 TGAAGTGGAACCAGCAACACC pLKO_005 215 CDS 100% 2.640 1.320 Y GAGE2A n/a
46 TRCN0000262815 AGCAACTCAACGTCAGGATCC pLKO_005 251 CDS 100% 2.250 1.125 Y GAGE2B n/a
47 TRCN0000255717 GAACCAGCAACTCAACGTCAG pLKO_005 246 CDS 100% 2.250 1.125 Y GAGE12F n/a
48 TRCN0000262875 ACTCAACGTCAGGATCCTGCA pLKO_005 255 CDS 100% 2.160 1.080 Y GAGE12J n/a
49 TRCN0000243319 AGTGATGAAGTGGAACCAGCA pLKO_005 210 CDS 100% 2.160 1.080 Y GAGE12I n/a
50 TRCN0000282455 CAGTGATGAAGTGGAACCAGC pLKO_005 209 CDS 100% 2.160 1.080 Y GAGE12J n/a
51 TRCN0000370885 GCCCGAGCAGTTCAGTGATGA pLKO_005 197 CDS 100% 1.650 0.825 Y GAGE6 n/a
52 TRCN0000369766 GGCCGAAGCCTGAAGCTGATA pLKO_005 319 CDS 100% 1.650 0.825 Y GAGE4 n/a
53 TRCN0000370819 CCGAAGCCTGAAGCTGATAGC pLKO_005 321 CDS 100% 1.350 0.675 Y GAGE6 n/a
54 TRCN0000243318 GGAGAGGATGAGGGAGCATCT pLKO_005 288 CDS 100% 1.350 0.675 Y GAGE12I n/a
55 TRCN0000412500 AGAGGATGAGGGAGCATCTGC pLKO_005 290 CDS 100% 0.880 0.440 Y GAGE2C n/a
56 TRCN0000371202 GCCTAGACCAAGACGCTACGT pLKO_005 146 CDS 100% 0.880 0.440 Y GAGE2A n/a
57 TRCN0000371148 GGGAACCAGCAACTCAACGTC pLKO_005 244 CDS 100% 0.880 0.440 Y GAGE2A n/a
58 TRCN0000432757 AGGATGAGGGAGCATCTGCAG pLKO_005 292 CDS 100% 0.720 0.360 Y GAGE7 n/a
59 TRCN0000371198 ATCTGCAGGTCAAGGGCCGAA pLKO_005 305 CDS 100% 0.720 0.360 Y GAGE2D n/a
60 TRCN0000415680 CAACGTCAGGATCCTGCAGCT pLKO_005 258 CDS 100% 0.720 0.360 Y GAGE2C n/a
61 TRCN0000371149 CTCAACGTCAGGATCCTGCAG pLKO_005 256 CDS 100% 0.720 0.360 Y GAGE2A n/a
62 TRCN0000370886 TCAACGTCAGGATCCTGCAGC pLKO_005 257 CDS 100% 0.720 0.360 Y GAGE6 n/a
63 TRCN0000262201 TGAAATGATTGGGCCTATGCG pLKO_005 176 CDS 100% 2.640 1.320 Y GAGE12D n/a
64 TRCN0000369765 GAAATGATTGGGCCTATGCGG pLKO_005 177 CDS 100% 0.660 0.330 Y GAGE4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001098413.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02972 pDONR223 100% 97.4% 93.9% None (many diffs) n/a
2 ccsbBroad304_02972 pLX_304 0% 97.4% 93.9% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000467585 CGTTGCGCGATTGGCGCAGTTACT pLX_317 88.8% 97.4% 93.9% V5 (many diffs) n/a
4 ccsbBroadEn_00610 pDONR223 100% 95.1% 90.5% None (many diffs) n/a
5 ccsbBroad304_00610 pLX_304 0% 95.1% 90.5% V5 (not translated due to frame shift) (many diffs) n/a
6 TRCN0000471710 CGGCTTATCACCGACTTTCCCGAT pLX_317 88.1% 95.1% 90.5% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_14111 pDONR223 100% 94.8% 86.3% None (many diffs) n/a
8 ccsbBroad304_14111 pLX_304 0% 94.8% 86.3% V5 (not translated due to frame shift) (many diffs) n/a
9 TRCN0000466573 TTGTGTATGCCTCTTGCCACGACC pLX_317 100% 94.8% 86.3% V5 (not translated due to frame shift) (many diffs) n/a
10 ccsbBroadEn_05736 pDONR223 100% 94.8% 89.7% None (many diffs) n/a
11 ccsbBroad304_05736 pLX_304 0% 94.8% 89.7% V5 (many diffs) n/a
12 TRCN0000467570 TATCCGGGAATCACCCTTACCCAG pLX_317 94.9% 94.8% 89.7% V5 (many diffs) n/a
13 ccsbBroadEn_06249 pDONR223 100% 94.5% 89.7% None (many diffs) n/a
14 ccsbBroad304_06249 pLX_304 0% 94.5% 89.7% V5 (many diffs) n/a
15 TRCN0000478101 CATCGACAATAGGTAGTCAAACGT pLX_317 40.9% 94.5% 89.7% V5 (many diffs) n/a
16 ccsbBroadEn_00603 pDONR223 100% 77.9% 72.8% None (many diffs) n/a
17 ccsbBroad304_00603 pLX_304 0% 77.9% 72.8% V5 (many diffs) n/a
18 TRCN0000466263 GTCGTATTTAGCCCTAATACTCGG pLX_317 96.3% 77.9% 72.8% V5 (many diffs) n/a
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