Transcript: Human NM_001127200.3

Homo sapiens G antigen 2E (GAGE2E), mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
GAGE2E (26749)
Length:
535
CDS:
94..444

Additional Resources:

NCBI RefSeq record:
NM_001127200.3
NBCI Gene record:
GAGE2E (26749)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001127200.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243155 CTACGTAGAGCCTCCTGAAAT pLKO_005 138 CDS 100% 13.200 6.600 Y GAGE2E n/a
2 TRCN0000265712 TACGTAGAGCCTCCTGAAATG pLKO_005 139 CDS 100% 10.800 5.400 Y GAGE2D n/a
3 TRCN0000265787 TAGAGCCTCCTGAAATGATTG pLKO_005 143 CDS 100% 10.800 5.400 Y GAGE2A n/a
4 TRCN0000197332 CCTGAAATGATTGGGCCTATG pLKO.1 151 CDS 100% 6.000 3.000 Y GAGE4 n/a
5 TRCN0000262814 ACGTAGAGCCTCCTGAAATGA pLKO_005 140 CDS 100% 5.625 2.813 Y GAGE2B n/a
6 TRCN0000138296 CGTAGAGCCTCCTGAAATGAT pLKO.1 141 CDS 100% 5.625 2.813 Y GAGE1 n/a
7 TRCN0000263271 GTAGAGCCTCCTGAAATGATT pLKO_005 142 CDS 100% 5.625 2.813 Y GAGE13 n/a
8 TRCN0000256038 ACTGGGTGTGAGTGTGAAGAT pLKO_005 340 CDS 100% 4.950 2.475 Y GAGE2D n/a
9 TRCN0000244667 CAGACTGGGTGTGAGTGTGAA pLKO_005 337 CDS 100% 4.950 2.475 Y GAGE4 n/a
10 TRCN0000137608 CCAAATCCAGAGGAGGTGAAA pLKO.1 388 CDS 100% 4.950 2.475 Y GAGE1 n/a
11 TRCN0000155993 CCAAATCCAGAGGAGGTGAAA pLKO.1 388 CDS 100% 4.950 2.475 Y GAGE2C n/a
12 TRCN0000115784 CCCGAGCAGTTCAGTGATGAA pLKO.1 175 CDS 100% 4.950 2.475 Y GAGE7 n/a
13 TRCN0000369735 CCGAGCAGTTCAGTGATGAAG pLKO_005 176 CDS 100% 4.950 2.475 Y GAGE4 n/a
14 TRCN0000138397 CGAGCAGTTCAGTGATGAAGT pLKO.1 177 CDS 100% 4.950 2.475 Y GAGE1 n/a
15 TRCN0000256139 CTGGGTGTGAGTGTGAAGATG pLKO_005 341 CDS 100% 4.950 2.475 Y GAGE12H n/a
16 TRCN0000137684 GAACCAGCAACACCTGAAGAA pLKO.1 199 CDS 100% 4.950 2.475 Y GAGE1 n/a
17 TRCN0000155031 GAACCAGCAACACCTGAAGAA pLKO.1 199 CDS 100% 4.950 2.475 Y GAGE2C n/a
18 TRCN0000419595 GCTACGTAGAGCCTCCTGAAA pLKO_005 137 CDS 100% 4.950 2.475 Y GAGE2C n/a
19 TRCN0000369730 GGAACCAGCAACACCTGAAGA pLKO_005 198 CDS 100% 4.950 2.475 Y GAGE4 n/a
20 TRCN0000371197 ACCAGCAACACCTGAAGAAGG pLKO_005 201 CDS 100% 4.050 2.025 Y GAGE2D n/a
21 TRCN0000263270 AGACCAAGACGCTACGTAGAG pLKO_005 127 CDS 100% 4.050 2.025 Y GAGE13 n/a
22 TRCN0000255889 AGACTGGGTGTGAGTGTGAAG pLKO_005 338 CDS 100% 4.050 2.025 Y GAGE12E n/a
23 TRCN0000243152 AGTGTGAAGATGGTCCTGATG pLKO_005 350 CDS 100% 4.050 2.025 Y GAGE2E n/a
24 TRCN0000256037 AGTTCAGTGATGAAGTGGAAC pLKO_005 182 CDS 100% 4.050 2.025 Y GAGE2D n/a
25 TRCN0000370840 ATGAAGTGGAACCAGCAACAC pLKO_005 191 CDS 100% 4.050 2.025 Y GAGE6 n/a
26 TRCN0000255388 CAGCAACTCAACGTCAGGATC pLKO_005 227 CDS 100% 4.050 2.025 Y GAGE10 n/a
27 TRCN0000243153 CTAGACCAAGACGCTACGTAG pLKO_005 125 CDS 100% 4.050 2.025 Y GAGE2E n/a
28 TRCN0000243156 CTGAAATGATTGGGCCTATGC pLKO_005 152 CDS 100% 4.050 2.025 Y GAGE2E n/a
29 TRCN0000243154 CTGAAGCTGATAGCCAGGAAC pLKO_005 305 CDS 100% 4.050 2.025 Y GAGE2E n/a
30 TRCN0000262876 GAAGCTGATAGCCAGGAACAG pLKO_005 307 CDS 100% 4.050 2.025 Y GAGE12J n/a
31 TRCN0000265695 GAGCAGTTCAGTGATGAAGTG pLKO_005 178 CDS 100% 4.050 2.025 Y GAGE12F n/a
32 TRCN0000115783 GAGTGTGAAGATGGTCCTGAT pLKO.1 349 CDS 100% 4.050 2.025 Y GAGE7 n/a
33 TRCN0000137973 GAGTGTGAAGATGGTCCTGAT pLKO.1 349 CDS 100% 4.050 2.025 Y GAGE1 n/a
34 TRCN0000155220 GATGAAGTGGAACCAGCAACA pLKO.1 190 CDS 100% 4.050 2.025 Y GAGE2C n/a
35 TRCN0000162881 GATGAAGTGGAACCAGCAACA pLKO.1 190 CDS 100% 4.050 2.025 Y GAGE12I n/a
36 TRCN0000243315 GCCAAATCCAGAGGAGGTGAA pLKO_005 387 CDS 100% 4.050 2.025 Y GAGE12I n/a
37 TRCN0000255386 GTGGAACCAGCAACACCTGAA pLKO_005 196 CDS 100% 4.050 2.025 Y GAGE10 n/a
38 TRCN0000265779 GTGTGAAGATGGTCCTGATGG pLKO_005 351 CDS 100% 4.050 2.025 Y GAGE2A n/a
39 TRCN0000282435 TAGACCAAGACGCTACGTAGA pLKO_005 126 CDS 100% 4.050 2.025 Y GAGE2B n/a
40 TRCN0000115782 TCCTGAAATGATTGGGCCTAT pLKO.1 150 CDS 100% 4.050 2.025 Y GAGE7 n/a
41 TRCN0000281477 TGAAGCTGATAGCCAGGAACA pLKO_005 306 CDS 100% 4.050 2.025 Y GAGE2D n/a
42 TRCN0000242734 TGATGAAGTGGAACCAGCAAC pLKO_005 189 CDS 100% 4.050 2.025 Y GAGE5 n/a
43 TRCN0000255718 TGGAACCAGCAACACCTGAAG pLKO_005 197 CDS 100% 4.050 2.025 Y GAGE12F n/a
44 TRCN0000432465 TGGGTGTGAGTGTGAAGATGG pLKO_005 342 CDS 100% 4.050 2.025 Y GAGE2C n/a
45 TRCN0000256336 AGGAGATGGACCCGCCAAATC pLKO_005 374 CDS 100% 3.600 1.800 Y GAGE2A n/a
46 TRCN0000243317 CAGGAGATGGACCCGCCAAAT pLKO_005 373 CDS 100% 3.600 1.800 Y GAGE12I n/a
47 TRCN0000242732 GCCGAAGCCTGAAGCTGATAG pLKO_005 297 CDS 100% 3.600 1.800 Y GAGE5 n/a
48 TRCN0000371199 CCTAGACCAAGACGCTACGTA pLKO_005 124 CDS 100% 3.000 1.500 Y GAGE2D n/a
49 TRCN0000262813 ACAGACTGGGTGTGAGTGTGA pLKO_005 336 CDS 100% 2.640 1.320 Y GAGE2B n/a
50 TRCN0000244666 ACCAGCAACTCAACGTCAGGA pLKO_005 225 CDS 100% 2.640 1.320 Y GAGE4 n/a
51 TRCN0000265767 AGCAGTTCAGTGATGAAGTGG pLKO_005 179 CDS 100% 2.640 1.320 Y GAGE12H n/a
52 TRCN0000265850 AGTGGAACCAGCAACACCTGA pLKO_005 195 CDS 100% 2.640 1.320 Y GAGE12E n/a
53 TRCN0000282344 CAACTCAACGTCAGGATCCTG pLKO_005 230 CDS 100% 2.640 1.320 Y GAGE12G n/a
54 TRCN0000121911 CAGTTCAGTGATGAAGTGGAA pLKO.1 181 CDS 100% 2.640 1.320 Y GAGE7 n/a
55 TRCN0000136873 CAGTTCAGTGATGAAGTGGAA pLKO.1 181 CDS 100% 2.640 1.320 Y GAGE1 n/a
56 TRCN0000115786 CCAGCAACTCAACGTCAGGAT pLKO.1 226 CDS 100% 2.640 1.320 Y GAGE7 n/a
57 TRCN0000263272 CCTGAAGCTGATAGCCAGGAA pLKO_005 304 CDS 100% 2.640 1.320 Y GAGE13 n/a
58 TRCN0000262618 GAAGTGGAACCAGCAACACCT pLKO_005 193 CDS 100% 2.640 1.320 Y GAGE12G n/a
59 TRCN0000281478 GCAACTCAACGTCAGGATCCT pLKO_005 229 CDS 100% 2.640 1.320 Y GAGE2D n/a
60 TRCN0000155329 GCAGTTCAGTGATGAAGTGGA pLKO.1 180 CDS 100% 2.640 1.320 Y GAGE2C n/a
61 TRCN0000250096 GGAACCAGCAACTCAACGTCA pLKO_005 222 CDS 100% 2.640 1.320 Y GAGE6 n/a
62 TRCN0000163548 GTGATGAAGTGGAACCAGCAA pLKO.1 188 CDS 100% 2.640 1.320 Y GAGE12I n/a
63 TRCN0000262201 TGAAATGATTGGGCCTATGCG pLKO_005 153 CDS 100% 2.640 1.320 Y GAGE12D n/a
64 TRCN0000265783 TGAAGTGGAACCAGCAACACC pLKO_005 192 CDS 100% 2.640 1.320 Y GAGE2A n/a
65 TRCN0000262812 TGAGTGTGAAGATGGTCCTGA pLKO_005 348 CDS 100% 2.640 1.320 Y GAGE2B n/a
66 TRCN0000262815 AGCAACTCAACGTCAGGATCC pLKO_005 228 CDS 100% 2.250 1.125 Y GAGE2B n/a
67 TRCN0000255717 GAACCAGCAACTCAACGTCAG pLKO_005 223 CDS 100% 2.250 1.125 Y GAGE12F n/a
68 TRCN0000262875 ACTCAACGTCAGGATCCTGCA pLKO_005 232 CDS 100% 2.160 1.080 Y GAGE12J n/a
69 TRCN0000243319 AGTGATGAAGTGGAACCAGCA pLKO_005 187 CDS 100% 2.160 1.080 Y GAGE12I n/a
70 TRCN0000282455 CAGTGATGAAGTGGAACCAGC pLKO_005 186 CDS 100% 2.160 1.080 Y GAGE12J n/a
71 TRCN0000138463 CTCCTGAAATGATTGGGCCTA pLKO.1 149 CDS 100% 2.160 1.080 Y GAGE1 n/a
72 TRCN0000262199 GTGAGTGTGAAGATGGTCCTG pLKO_005 347 CDS 100% 2.160 1.080 Y GAGE12D n/a
73 TRCN0000281576 TGTGAAGATGGTCCTGATGGG pLKO_005 352 CDS 100% 2.160 1.080 Y GAGE12C n/a
74 TRCN0000370885 GCCCGAGCAGTTCAGTGATGA pLKO_005 174 CDS 100% 1.650 0.825 Y GAGE6 n/a
75 TRCN0000369766 GGCCGAAGCCTGAAGCTGATA pLKO_005 296 CDS 100% 1.650 0.825 Y GAGE4 n/a
76 TRCN0000370819 CCGAAGCCTGAAGCTGATAGC pLKO_005 298 CDS 100% 1.350 0.675 Y GAGE6 n/a
77 TRCN0000243318 GGAGAGGATGAGGGAGCATCT pLKO_005 265 CDS 100% 1.350 0.675 Y GAGE12I n/a
78 TRCN0000412836 GAGATGGACCCGCCAAATCCA pLKO_005 376 CDS 100% 1.000 0.500 Y GAGE2C n/a
79 TRCN0000412500 AGAGGATGAGGGAGCATCTGC pLKO_005 267 CDS 100% 0.880 0.440 Y GAGE2C n/a
80 TRCN0000371145 AGGAACAGGGTCACCCACAGA pLKO_005 320 CDS 100% 0.880 0.440 Y GAGE2D n/a
81 TRCN0000371202 GCCTAGACCAAGACGCTACGT pLKO_005 123 CDS 100% 0.880 0.440 Y GAGE2A n/a
82 TRCN0000371148 GGGAACCAGCAACTCAACGTC pLKO_005 221 CDS 100% 0.880 0.440 Y GAGE2A n/a
83 TRCN0000432757 AGGATGAGGGAGCATCTGCAG pLKO_005 269 CDS 100% 0.720 0.360 Y GAGE7 n/a
84 TRCN0000371198 ATCTGCAGGTCAAGGGCCGAA pLKO_005 282 CDS 100% 0.720 0.360 Y GAGE2D n/a
85 TRCN0000415680 CAACGTCAGGATCCTGCAGCT pLKO_005 235 CDS 100% 0.720 0.360 Y GAGE2C n/a
86 TRCN0000371149 CTCAACGTCAGGATCCTGCAG pLKO_005 233 CDS 100% 0.720 0.360 Y GAGE2A n/a
87 TRCN0000370886 TCAACGTCAGGATCCTGCAGC pLKO_005 234 CDS 100% 0.720 0.360 Y GAGE6 n/a
88 TRCN0000369765 GAAATGATTGGGCCTATGCGG pLKO_005 154 CDS 100% 0.660 0.330 Y GAGE4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001127200.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02972 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02972 pLX_304 0% 100% 100% V5 (not translated due to frame shift) n/a
3 TRCN0000467585 CGTTGCGCGATTGGCGCAGTTACT pLX_317 88.8% 100% 100% V5 n/a
4 ccsbBroadEn_00610 pDONR223 100% 97.7% 96.5% None (many diffs) n/a
5 ccsbBroad304_00610 pLX_304 0% 97.7% 96.5% V5 (not translated due to frame shift) (many diffs) n/a
6 TRCN0000471710 CGGCTTATCACCGACTTTCCCGAT pLX_317 88.1% 97.7% 96.5% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_14111 pDONR223 100% 97.4% 92.3% None (many diffs) n/a
8 ccsbBroad304_14111 pLX_304 0% 97.4% 92.3% V5 (not translated due to frame shift) (many diffs) n/a
9 TRCN0000466573 TTGTGTATGCCTCTTGCCACGACC pLX_317 100% 97.4% 92.3% V5 (not translated due to frame shift) (many diffs) n/a
10 ccsbBroadEn_05736 pDONR223 100% 97.4% 95.7% None (many diffs) n/a
11 ccsbBroad304_05736 pLX_304 0% 97.4% 95.7% V5 (many diffs) n/a
12 TRCN0000467570 TATCCGGGAATCACCCTTACCCAG pLX_317 94.9% 97.4% 95.7% V5 (many diffs) n/a
13 ccsbBroadEn_06249 pDONR223 100% 97.1% 95.7% None (many diffs) n/a
14 ccsbBroad304_06249 pLX_304 0% 97.1% 95.7% V5 (many diffs) n/a
15 TRCN0000478101 CATCGACAATAGGTAGTCAAACGT pLX_317 40.9% 97.1% 95.7% V5 (many diffs) n/a
16 ccsbBroadEn_00603 pDONR223 100% 80.1% 77.8% None (many diffs) n/a
17 ccsbBroad304_00603 pLX_304 0% 80.1% 77.8% V5 (many diffs) n/a
18 TRCN0000466263 GTCGTATTTAGCCCTAATACTCGG pLX_317 96.3% 80.1% 77.8% V5 (many diffs) n/a
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