Transcript: Human XM_024452325.1

PREDICTED: Homo sapiens G antigen 10 (GAGE10), transcript variant X1, partial mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GAGE10 (102724473)
Length:
403
CDS:
1..309

Additional Resources:

NCBI RefSeq record:
XM_024452325.1
NBCI Gene record:
GAGE10 (102724473)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024452325.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000255387 AGGAACAGGTTCACCCAAAGA pLKO_005 185 CDS 100% 4.950 2.970 N GAGE10 n/a
2 TRCN0000137608 CCAAATCCAGAGGAGGTGAAA pLKO.1 253 CDS 100% 4.950 2.475 Y GAGE1 n/a
3 TRCN0000155993 CCAAATCCAGAGGAGGTGAAA pLKO.1 253 CDS 100% 4.950 2.475 Y GAGE2C n/a
4 TRCN0000115784 CCCGAGCAGTTCAGTGATGAA pLKO.1 40 CDS 100% 4.950 2.475 Y GAGE7 n/a
5 TRCN0000369735 CCGAGCAGTTCAGTGATGAAG pLKO_005 41 CDS 100% 4.950 2.475 Y GAGE4 n/a
6 TRCN0000138397 CGAGCAGTTCAGTGATGAAGT pLKO.1 42 CDS 100% 4.950 2.475 Y GAGE1 n/a
7 TRCN0000137684 GAACCAGCAACACCTGAAGAA pLKO.1 64 CDS 100% 4.950 2.475 Y GAGE1 n/a
8 TRCN0000155031 GAACCAGCAACACCTGAAGAA pLKO.1 64 CDS 100% 4.950 2.475 Y GAGE2C n/a
9 TRCN0000369730 GGAACCAGCAACACCTGAAGA pLKO_005 63 CDS 100% 4.950 2.475 Y GAGE4 n/a
10 TRCN0000371197 ACCAGCAACACCTGAAGAAGG pLKO_005 66 CDS 100% 4.050 2.025 Y GAGE2D n/a
11 TRCN0000256037 AGTTCAGTGATGAAGTGGAAC pLKO_005 47 CDS 100% 4.050 2.025 Y GAGE2D n/a
12 TRCN0000370840 ATGAAGTGGAACCAGCAACAC pLKO_005 56 CDS 100% 4.050 2.025 Y GAGE6 n/a
13 TRCN0000255388 CAGCAACTCAACGTCAGGATC pLKO_005 92 CDS 100% 4.050 2.025 Y GAGE10 n/a
14 TRCN0000243154 CTGAAGCTGATAGCCAGGAAC pLKO_005 170 CDS 100% 4.050 2.025 Y GAGE2E n/a
15 TRCN0000262876 GAAGCTGATAGCCAGGAACAG pLKO_005 172 CDS 100% 4.050 2.025 Y GAGE12J n/a
16 TRCN0000265695 GAGCAGTTCAGTGATGAAGTG pLKO_005 43 CDS 100% 4.050 2.025 Y GAGE12F n/a
17 TRCN0000155220 GATGAAGTGGAACCAGCAACA pLKO.1 55 CDS 100% 4.050 2.025 Y GAGE2C n/a
18 TRCN0000162881 GATGAAGTGGAACCAGCAACA pLKO.1 55 CDS 100% 4.050 2.025 Y GAGE12I n/a
19 TRCN0000243315 GCCAAATCCAGAGGAGGTGAA pLKO_005 252 CDS 100% 4.050 2.025 Y GAGE12I n/a
20 TRCN0000255386 GTGGAACCAGCAACACCTGAA pLKO_005 61 CDS 100% 4.050 2.025 Y GAGE10 n/a
21 TRCN0000281477 TGAAGCTGATAGCCAGGAACA pLKO_005 171 CDS 100% 4.050 2.025 Y GAGE2D n/a
22 TRCN0000242734 TGATGAAGTGGAACCAGCAAC pLKO_005 54 CDS 100% 4.050 2.025 Y GAGE5 n/a
23 TRCN0000255718 TGGAACCAGCAACACCTGAAG pLKO_005 62 CDS 100% 4.050 2.025 Y GAGE12F n/a
24 TRCN0000242732 GCCGAAGCCTGAAGCTGATAG pLKO_005 162 CDS 100% 3.600 1.800 Y GAGE5 n/a
25 TRCN0000244666 ACCAGCAACTCAACGTCAGGA pLKO_005 90 CDS 100% 2.640 1.320 Y GAGE4 n/a
26 TRCN0000265767 AGCAGTTCAGTGATGAAGTGG pLKO_005 44 CDS 100% 2.640 1.320 Y GAGE12H n/a
27 TRCN0000265850 AGTGGAACCAGCAACACCTGA pLKO_005 60 CDS 100% 2.640 1.320 Y GAGE12E n/a
28 TRCN0000282344 CAACTCAACGTCAGGATCCTG pLKO_005 95 CDS 100% 2.640 1.320 Y GAGE12G n/a
29 TRCN0000121911 CAGTTCAGTGATGAAGTGGAA pLKO.1 46 CDS 100% 2.640 1.320 Y GAGE7 n/a
30 TRCN0000136873 CAGTTCAGTGATGAAGTGGAA pLKO.1 46 CDS 100% 2.640 1.320 Y GAGE1 n/a
31 TRCN0000115786 CCAGCAACTCAACGTCAGGAT pLKO.1 91 CDS 100% 2.640 1.320 Y GAGE7 n/a
32 TRCN0000263272 CCTGAAGCTGATAGCCAGGAA pLKO_005 169 CDS 100% 2.640 1.320 Y GAGE13 n/a
33 TRCN0000262618 GAAGTGGAACCAGCAACACCT pLKO_005 58 CDS 100% 2.640 1.320 Y GAGE12G n/a
34 TRCN0000281478 GCAACTCAACGTCAGGATCCT pLKO_005 94 CDS 100% 2.640 1.320 Y GAGE2D n/a
35 TRCN0000155329 GCAGTTCAGTGATGAAGTGGA pLKO.1 45 CDS 100% 2.640 1.320 Y GAGE2C n/a
36 TRCN0000250096 GGAACCAGCAACTCAACGTCA pLKO_005 87 CDS 100% 2.640 1.320 Y GAGE6 n/a
37 TRCN0000163548 GTGATGAAGTGGAACCAGCAA pLKO.1 53 CDS 100% 2.640 1.320 Y GAGE12I n/a
38 TRCN0000265783 TGAAGTGGAACCAGCAACACC pLKO_005 57 CDS 100% 2.640 1.320 Y GAGE2A n/a
39 TRCN0000262815 AGCAACTCAACGTCAGGATCC pLKO_005 93 CDS 100% 2.250 1.125 Y GAGE2B n/a
40 TRCN0000255717 GAACCAGCAACTCAACGTCAG pLKO_005 88 CDS 100% 2.250 1.125 Y GAGE12F n/a
41 TRCN0000262875 ACTCAACGTCAGGATCCTGCA pLKO_005 97 CDS 100% 2.160 1.080 Y GAGE12J n/a
42 TRCN0000243319 AGTGATGAAGTGGAACCAGCA pLKO_005 52 CDS 100% 2.160 1.080 Y GAGE12I n/a
43 TRCN0000282455 CAGTGATGAAGTGGAACCAGC pLKO_005 51 CDS 100% 2.160 1.080 Y GAGE12J n/a
44 TRCN0000370885 GCCCGAGCAGTTCAGTGATGA pLKO_005 39 CDS 100% 1.650 0.825 Y GAGE6 n/a
45 TRCN0000369766 GGCCGAAGCCTGAAGCTGATA pLKO_005 161 CDS 100% 1.650 0.825 Y GAGE4 n/a
46 TRCN0000370819 CCGAAGCCTGAAGCTGATAGC pLKO_005 163 CDS 100% 1.350 0.675 Y GAGE6 n/a
47 TRCN0000243318 GGAGAGGATGAGGGAGCATCT pLKO_005 130 CDS 100% 1.350 0.675 Y GAGE12I n/a
48 TRCN0000412500 AGAGGATGAGGGAGCATCTGC pLKO_005 132 CDS 100% 0.880 0.440 Y GAGE2C n/a
49 TRCN0000371148 GGGAACCAGCAACTCAACGTC pLKO_005 86 CDS 100% 0.880 0.440 Y GAGE2A n/a
50 TRCN0000432757 AGGATGAGGGAGCATCTGCAG pLKO_005 134 CDS 100% 0.720 0.360 Y GAGE7 n/a
51 TRCN0000371198 ATCTGCAGGTCAAGGGCCGAA pLKO_005 147 CDS 100% 0.720 0.360 Y GAGE2D n/a
52 TRCN0000415680 CAACGTCAGGATCCTGCAGCT pLKO_005 100 CDS 100% 0.720 0.360 Y GAGE2C n/a
53 TRCN0000371149 CTCAACGTCAGGATCCTGCAG pLKO_005 98 CDS 100% 0.720 0.360 Y GAGE2A n/a
54 TRCN0000370886 TCAACGTCAGGATCCTGCAGC pLKO_005 99 CDS 100% 0.720 0.360 Y GAGE6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024452325.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00610 pDONR223 100% 82.3% 73.5% None (many diffs) n/a
2 ccsbBroad304_00610 pLX_304 0% 82.3% 73.5% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000471710 CGGCTTATCACCGACTTTCCCGAT pLX_317 88.1% 82.3% 73.5% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_14111 pDONR223 100% 80% 68.3% None (many diffs) n/a
5 ccsbBroad304_14111 pLX_304 0% 80% 68.3% V5 (not translated due to frame shift) (many diffs) n/a
6 TRCN0000466573 TTGTGTATGCCTCTTGCCACGACC pLX_317 100% 80% 68.3% V5 (not translated due to frame shift) (many diffs) n/a
7 ccsbBroadEn_05736 pDONR223 100% 80% 71.7% None (many diffs) n/a
8 ccsbBroad304_05736 pLX_304 0% 80% 71.7% V5 (many diffs) n/a
9 TRCN0000467570 TATCCGGGAATCACCCTTACCCAG pLX_317 94.9% 80% 71.7% V5 (many diffs) n/a
10 ccsbBroadEn_06249 pDONR223 100% 79.7% 71.7% None (many diffs) n/a
11 ccsbBroad304_06249 pLX_304 0% 79.7% 71.7% V5 (many diffs) n/a
12 TRCN0000478101 CATCGACAATAGGTAGTCAAACGT pLX_317 40.9% 79.7% 71.7% V5 (many diffs) n/a
13 ccsbBroadEn_02972 pDONR223 100% 77.9% 73.2% None (many diffs) n/a
14 ccsbBroad304_02972 pLX_304 0% 77.9% 73.2% V5 (not translated due to frame shift) (many diffs) n/a
15 TRCN0000467585 CGTTGCGCGATTGGCGCAGTTACT pLX_317 88.8% 77.9% 73.2% V5 (many diffs) n/a
16 ccsbBroadEn_00603 pDONR223 100% 65.6% 57.8% None (many diffs) n/a
17 ccsbBroad304_00603 pLX_304 0% 65.6% 57.8% V5 (many diffs) n/a
18 TRCN0000466263 GTCGTATTTAGCCCTAATACTCGG pLX_317 96.3% 65.6% 57.8% V5 (many diffs) n/a
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