Construct: ORF ccsbBroad304_03189
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005942.1_s304c1, BRDN0000400136
- Derived from:
- ccsbBroadEn_03189
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NMD3 (51068)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03189
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51068 | NMD3 | NMD3 ribosome export adaptor | NM_015938.4 | 100% | 100% | |
| 2 | human | 51068 | NMD3 | NMD3 ribosome export adaptor | XM_005247511.2 | 100% | 100% | |
| 3 | human | 51068 | NMD3 | NMD3 ribosome export adaptor | XM_005247512.1 | 100% | 100% | |
| 4 | human | 51068 | NMD3 | NMD3 ribosome export adaptor | NM_001320227.1 | 94.9% | 94.1% | (many diffs) |
| 5 | human | 51068 | NMD3 | NMD3 ribosome export adaptor | XR_001740167.2 | 47.2% | 1_60del;1076_1077ins113;1457_2843del | |
| 6 | human | 51068 | NMD3 | NMD3 ribosome export adaptor | XR_001740166.2 | 46% | 1_137del;1153_1154ins113;1534_2920del | |
| 7 | mouse | 97112 | Nmd3 | NMD3 ribosome export adaptor | NM_133787.2 | 91.3% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1575
- ORF length:
- 1509
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gtatatggca gaatccaccg accgcagccc tggacacatc ttgtgctgtg 121 agtgtggtgt tccgataagt ccaaatcctg ccaatatttg tgtggcctgt ttgcgaagta 181 aagtggacat cagccaaggt attccgaaac aagtctcgat ttcgttctgc aaacaatgtc 241 aaaggtattt tcaaccacca ggaacttgga tacagtgtgc tttagaatcc agggaacttc 301 ttgctttgtg cttgaaaaaa atcaaagccc ctctgagtaa ggtacggctt gtagatgcag 361 gctttgtttg gactgagcct cattctaaga gacttaaagt taaactgact attcagaaag 421 aggtgatgaa tggtgctatc cttcaacaag tgtttgtggt ggattatgtt gttcagtccc 481 aaatgtgtgg agattgccat agagtagaag ctaaggattt ctggaaggct gtgattcaag 541 tgaggcaaaa gactttgcat aaaaaaactt tctactatct ggaacagtta attctgaaat 601 atggaatgca tcagaataca cttcgtatca aagagattca tgatggtctg gatttttatt 661 attcctcaaa acaacatgct cagaagatgg tcgaatttct tcagtgtaca gttccctgta 721 gatacaaagc atcacaaaga ctgatctctc aagatatcca tagtaacaca tacaattaca 781 aaagcacttt ttctgtggaa attgttccaa tatgcaagga taatgttgtc tgtctgtctc 841 caaaactggc acaaagcctg ggaaatatga accagatttg tgtgtgtatt cgagtaacca 901 gtgccattca cctcattgat ccaaacaccc tacaagtggc agatattgat gggagcactt 961 tctggagtca ccctttcaat agtttatgtc atcccaaaca gctagaggag tttattgtga 1021 tggaatgcag catagtccaa gatataaaac gtgctgcagg tgctggaatg atatcaaaaa 1081 agcataccct cggggaagtc tgggtacaga agacatctga aatgaataca gataaacagt 1141 atttttgtcg tactcatttg ggacatcttc taaatcccgg agacctggtg ttagggtttg 1201 atttggccaa ctgtaactta aatgatgagc atgtcaacaa aatgaactca gatagagttc 1261 cagatgtggt attaatcaag aagagctatg accggaccaa acgtcagcgt cgtagaaact 1321 ggaaattgaa agagcttgca agagagagag aaaacatgga tacagatgat gaaaggcaat 1381 accaagattt tcttgaagat cttgaagaag atgaggcaat tcgaaaaaat gtcaacattt 1441 acagagattc agccatccct gtggaaagtg acaccgatga tgaaggagca cctcgaatta 1501 gtctggctga gatgcttgaa gaccttcata tttcccaaga tgccactggt gaagaaggtg 1561 catcaatgct gacataccca actttcttgt acaaagtggt tggtaagcct atccctaacc 1621 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1681 cgacaatcaa cctctggatt a