Construct: ORF ccsbBroad304_03462

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014210.1_s304c1, BRDN0000408659
Derived from:
ccsbBroadEn_03462
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLHL28 (54813)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03462
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54813 KLHL28 kelch like family member 28 NM_017658.5 100% 100%
2 human 54813 KLHL28 kelch like family member 28 XM_005267770.4 100% 100%
3 human 54813 KLHL28 kelch like family member 28 XM_011536847.3 100% 100%
4 human 54813 KLHL28 kelch like family member 28 NM_001308112.2 97.6% 97.6% 1_42del
5 human 54813 KLHL28 kelch like family member 28 XM_024449635.1 78.9% 77.9% (many diffs)
6 human 54813 KLHL28 kelch like family member 28 XM_011536849.2 77% 76% (many diffs)
7 human 54813 KLHL28 kelch like family member 28 XM_017021378.1 53.7% 52.8% (many diffs)
8 human 54813 KLHL28 kelch like family member 28 XM_006720174.3 52.4% 51.6% (many diffs)
9 mouse 66689 Klhl28 kelch-like 28 NM_025707.3 91.1% 97% (many diffs)
10 mouse 66689 Klhl28 kelch-like 28 XM_006516156.3 91.1% 97% (many diffs)
11 mouse 66689 Klhl28 kelch-like 28 XM_006516157.3 86.5% 89.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1782
ORF length:
1713
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaccacaca tccccgacct acatgcttgc taacttaacc cacttgcatt
121ctgaacaact tctgcagggc ttgaatcttc ttcgccaaca tcacgaactc tgtgacatca
181ttcttcgagt aggtgatgtt aaaattcatg ctcacaaagt ggtacttgcc agcgtcagcc
241cgtatttcaa agctatgttc actggaaacc tttctgaaaa agagaacagt gaggttgagt
301ttcaatgcat tgatgaaact gctctccagg ccattgtgga gtatgcctat acagggactg
361tttttatttc tcaggacaca gttgaatctc tcctgccagc agcaaaccta ctccagataa
421aacttgtcct gaaagaatgt tgtgcatttc ttgaaagcca acttgatcct ggtaattgta
481ttggaatttc tcgttttgca gaaacatatg gttgccgtga cctttatttg gcagccacta
541aatacatatg ccagaatttt gaagctgttt gccagactga agagtttttt gagcttacac
601atgctgactt ggatgaaatt gtttccaatg actgtttgaa tgtagctacc gaagagactg
661ttttttatgc attagagtct tggatcaagt atgatgtaca agaacgccag aaatacttag
721cacagttact aaacagtgta cgattaccat tgttgagtgt taagtttctc actagactat
781atgaagcaaa tcatcttatt cgtgatgatc gcacttgtaa acatcttttg aatgaagccc
841taaagtacca ctttatgcct gaacatagac tctctcatca gacagtcttg atgacacgac
901ctcgctgtgc tcccaaagta ctttgtgcag taggagggaa atctggactc tttgcctgtt
961tggatagtgt ggagatgtac tttcctcaga atgactcttg gattggtttg gcacccctaa
1021acattcctcg ctatgaattt ggaatatgcg ttttagacca aaaagtatat gttataggtg
1081gtattgcaac taatgtgcgt cctggcgtca ctatcagaaa acatgaaaat tcagtggaat
1141gctggaatcc tgatacaaat acttggactt ctctagagag aatgaatgaa agccgaagta
1201ctcttggagt agtagtactt gcaggagaac tttatgcctt aggtggttat gatggacaat
1261cttatttaca atctgtagag aagtacattc ccaaaataag aaaatggcaa cctgtggcac
1321caatgacgac aacaagaagt tgttttgctg cagcggtatt ggatggaatg atatatgcca
1381ttggtgggta tggtcctgcc cacatgaaca gtgtggagcg ttatgatcca agtaaggact
1441cctgggagat ggttgcatcc atggcagata aaaggattca ctttggcgtg ggtgtcatgc
1501taggctttat ttttgtggtg ggtggacata atggagtctc acatttgtcc agcattgaaa
1561gatacgatcc tcatcaaaat cagtggactg tgtgtagacc aatgaaagaa cctagaacag
1621gagttggtgc tgctgtaatc gataactacc tttatgtcgt cggtggtcac tcagggtctt
1681cctatctgaa tacagtgcag aaatatgatc ctatctcaga tacgtggctg gattcagctg
1741gcatgatata ctgtcgctgc aactttgggt taactgcact tttgccaact ttcttgtaca
1801aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1861ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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