Construct: ORF ccsbBroad304_03845
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015946.1_s304c1, BRDN0000412032
- Derived from:
- ccsbBroadEn_03845
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIER1 (57708)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03845
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001146113.1 | 100% | 100% | |
| 2 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001077704.3 | 85.4% | 85.4% | 1_189del |
| 3 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001077702.3 | 82.2% | 82.2% | 1_240del |
| 4 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001932.1 | 81% | 75.8% | (many diffs) |
| 5 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001933.1 | 81% | 75.8% | (many diffs) |
| 6 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001934.1 | 81% | 75.8% | (many diffs) |
| 7 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001146111.1 | 78.7% | 78.7% | 1_240del;1280_1339del |
| 8 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001077703.3 | 76.1% | 76.1% | 1_348del |
| 9 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001146112.2 | 75% | 70.3% | (many diffs) |
| 10 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001931.1 | 75% | 70.3% | (many diffs) |
| 11 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001927.1 | 71.2% | 66.8% | (many diffs) |
| 12 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001928.2 | 71.2% | 66.8% | (many diffs) |
| 13 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001929.1 | 71.2% | 66.8% | (many diffs) |
| 14 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001930.1 | 71.2% | 66.8% | (many diffs) |
| 15 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001077701.3 | 71.1% | 66.7% | (many diffs) |
| 16 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001146110.2 | 68.8% | 64.6% | (many diffs) |
| 17 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_020948.4 | 68.8% | 64.6% | (many diffs) |
| 18 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_005271076.3 | 68.8% | 64.6% | (many diffs) |
| 19 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001924.1 | 68.8% | 64.6% | (many diffs) |
| 20 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001925.1 | 68.8% | 64.6% | (many diffs) |
| 21 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001926.1 | 68.8% | 64.6% | (many diffs) |
| 22 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001923.1 | 67.3% | 63.2% | (many diffs) |
| 23 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001921.1 | 66.8% | 62.7% | (many diffs) |
| 24 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001922.1 | 66.8% | 62.7% | (many diffs) |
| 25 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001919.1 | 66.4% | 62.4% | (many diffs) |
| 26 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_017001920.1 | 66.4% | 62.4% | (many diffs) |
| 27 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001077700.3 | 64.4% | 60.5% | (many diffs) |
| 28 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XM_011541865.2 | 61.8% | 58.1% | (many diffs) |
| 29 | human | 57708 | MIER1 | MIER1 transcriptional regul... | NM_001350530.2 | 60.5% | 56.9% | (many diffs) |
| 30 | human | 57708 | MIER1 | MIER1 transcriptional regul... | XR_001737345.1 | 20% | (many diffs) | |
| 31 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | XM_017320386.1 | 68.2% | 67.7% | (many diffs) |
| 32 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | NM_001286223.1 | 64.7% | 64.3% | (many diffs) |
| 33 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | NM_001039081.2 | 64.6% | 64.2% | (many diffs) |
| 34 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | NM_001286222.1 | 62.5% | 62.2% | (many diffs) |
| 35 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | NM_027696.3 | 62.5% | 62.2% | (many diffs) |
| 36 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | XM_006503401.3 | 62.5% | 62.2% | (many diffs) |
| 37 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | XM_006503402.1 | 62.5% | 62.2% | (many diffs) |
| 38 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | NM_001286221.1 | 59.4% | 59.1% | (many diffs) |
| 39 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | XM_006503399.2 | 58.8% | 58.5% | (many diffs) |
| 40 | mouse | 71148 | Mier1 | MEIR1 treanscription regulator | XM_006503398.2 | 57.2% | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1179
- ORF length:
- 1110
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gccaattcat gaacttctca gcctttatgg ttatggtagt actgttcgac 121 tacctgaaga agatgaggaa gaggaagaag aggaagaaga aggtgaagat gatgaagatg 181 ctgataatga tgacaacagt ggctgtagtg gggaaaataa agaggagaat ataaaggatt 241 catcaggtca ggaggatgaa actcagtctt ccaatgatga tccatcacaa tctgttgctt 301 ctcaagatgc ccaggaaata atccgcccac gtcgatgtaa atattttgat acaaatagtg 361 aagtagaaga agaatctgaa gaagatgaag attatattcc atcagaagac tggaaaaagg 421 agattatggt gggctccatg tttcaagcag aaattccagt tggcatttgt agatacaaag 481 aaaatgaaaa agtatatgaa aatgatgatc agctcctgtg ggaccctgag tacttaccag 541 aagataaagt gattatattt cttaaagatg catctagaag aacaggtgat gagaagggtg 601 tagaagcaat tcctgaagga tctcacataa aagacaatga acaggcttta tatgaattgg 661 ttaaatgcaa ttttgataca gaagaagcat tgagaagatt aagatttaat gtaaaagcag 721 ctagagagga attatctgtt tggacagagg aagagtgtag aaattttgaa caagggctga 781 aggcctatgg aaaggatttt catttgattc aggctaataa agtccgaaca aggtcagttg 841 gtgaatgtgt agcattctat tacatgtgga aaaaatctga acgttatgat ttctttgctc 901 agcaaacacg atttggaaag aagaaatata atcttcatcc tggtgtaacg gattacatgg 961 atcgtcttct agacgaaagt gaaagtgctg catctagtcg agcaccatcc cctcccccaa 1021 ctgcatcaaa cagtagtaac agccagtctg agaaagaaga tggcactgta agcactgcta 1081 atcaaaatgg agtgtcatct aatggaccag gcatactcca aatgcttctt ccagttcatt 1141 tttcagccat cagttcaaga gccaatgcct ttttaaaatt gccaactttc ttgtacaaag 1201 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1261 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta