Construct: ORF ccsbBroad304_03868
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004481.1_s304c1, BRDN0000462152
- Derived from:
- ccsbBroadEn_03868
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM25 (58517)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03868
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58517 | RBM25 | RNA binding motif protein 25 | NM_021239.3 | 100% | 100% | |
| 2 | human | 58517 | RBM25 | RNA binding motif protein 25 | XM_011537044.3 | 100% | 100% | |
| 3 | human | 58517 | RBM25 | RNA binding motif protein 25 | XM_024449680.1 | 75.6% | 75.6% | 0_1ins615 |
| 4 | human | 58517 | RBM25 | RNA binding motif protein 25 | XR_943501.3 | 55.5% | 1_189del;2206_2424del;2938_4549del | |
| 5 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | NM_027349.3 | 92% | 99.1% | (many diffs) |
| 6 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | XM_006516167.3 | 92% | 99.1% | (many diffs) |
| 7 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | XM_006516168.3 | 92% | 99.1% | (many diffs) |
| 8 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | XM_006516164.3 | 89.9% | 96.9% | (many diffs) |
| 9 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | XM_006516165.3 | 89.9% | 96.9% | (many diffs) |
| 10 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | XM_006516166.3 | 89.9% | 96.9% | (many diffs) |
| 11 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | XM_017315161.1 | 89.9% | 96.9% | (many diffs) |
| 12 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | XM_006516169.3 | 70% | 75% | (many diffs) |
| 13 | mouse | 67039 | Rbm25 | RNA binding motif protein 25 | XM_017315162.1 | 70% | 75% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2598
- ORF length:
- 2529
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcttttcca cctcatttga atcgccctcc catgggaatc ccagcactcc 121 caccagggat cccacccccg cagtttccag gatttcctcc acctgtacct ccagggaccc 181 caatgattcc tgtaccaatg agcattatgg ctcctgctcc aactgtctta gtacccactg 241 tgtctatggt tggaaagcat ttgggcgcaa gaaaggatca tccaggctta aaggctaaag 301 aaaatgatga aaattgtggt cctactacca ctgtttttgt tggcaacatt tccgagaaag 361 cttcagacat gcttataaga caactcttag ctaaatgtgg tttggttttg agctggaaga 421 gagtacaagg tgcttccgga aagcttcaag ccttcggatt ctgtgagtac aaggagccag 481 aatctaccct ccgtgcactc agattattac atgacctgca aattggagag aaaaagctac 541 tcgttaaagt tgatgcaaag acaaaggcac agctggatga atggaaagca aagaagaaag 601 cttctaatgg gaatgcaagg ccagaaactg tcactaatga cgatgaagaa gccttggatg 661 aagaaacaaa gaggagagat cagatgatta aaggggctat tgaagtttta attcgtgaat 721 actccagtga gctaaatgcc ccctcacagg aatctgattc tcaccccagg aagaagaaga 781 aggaaaagaa ggaggacatt ttccgcagat ttccagtggc cccactgatc ccttatccac 841 tcatcactaa ggaggatata aatgctatag aaatggaaga agacaaaaga gacctgatat 901 ctcgagagat cagcaaattc agagacacac ataagaaact ggaagaagag aaaggcaaaa 961 aggaaaaaga aagacaggaa attgagaaag aacggagaga aagagagagg gagcgtgaaa 1021 gggaacgaga aaggcgagaa cgggaacgag aaagggaaag agaacgtgaa cgagaaaagg 1081 agaaagaacg ggagcgggaa cgagaacggg atagggaccg tgaccggaca aaagagagag 1141 accgagatcg ggatcgagag agagatcgtg accgggatag agaaaggagc tcagatcgta 1201 ataaggatcg cagtcgatca agagaaaaaa gcagagatcg tgaaagggaa cgagagcggg 1261 aaagagagag agagagagaa cgagagcgag aacgagaacg ggagcgagag agagagcgag 1321 agagggaacg ggagcgagaa agagaaaaag acaaaaaacg ggaccgagaa gaagatgaag 1381 aagatgcata cgaacgaaga aaacttgaaa gaaaactccg agagaaagaa gctgcttatc 1441 aagagcgcct taagaattgg gaaatcagag aacgaaagaa aacccgggaa tatgagaaag 1501 aagctgaaag agaagaagaa agaagaagag aaatggccaa agaagctaaa cgactaaaag 1561 aattcttaga agactatgat gatgatagag atgaccccaa atattacaga ggaagtgctc 1621 ttcagaaaag gttgcgtgat agagaaaagg aaatggaagc agatgaacga gataggaaga 1681 gagagaagga ggagcttgag gaaatcaggc agcgccttct ggcagaaggg catccagatc 1741 cagatgcaga gctccagagg atggaacaag aggctgagag gcgcaggcag ccacaaataa 1801 agcaagagcc agaatcagaa gaggaggaag aagaaaagca agaaaaagaa gaaaaacgag 1861 aagaacccat ggaagaggaa gaggagccag agcaaaagcc ttgtctgaaa cctactctga 1921 ggcccatcag ctctgctcca tctgtttcct ctgccagtgg caatgcaaca cctaacactc 1981 ctggggatga gtctccctgt ggtattatta ttcctcatga aaactcacca gatcaacagc 2041 aacctgagga gcataggcca aaaataggac taagtcttaa actgggtgct tccaatagtc 2101 ctggtcagcc taattctgtg aagagaaaga aactacctgt agatagtgtc tttaacaaat 2161 ttgaggatga agacagtgat gacgtacccc gaaaaaggaa actggttccc ttggattatg 2221 gtgaagatga taaaaatgca accaaaggca ctgtaaacac tgaagaaaag cgtaaacaca 2281 ttaagagtct cattgagaaa atccctacag ccaaacctga gctcttcgct tatcccctgg 2341 attggtctat tgtggattct atactgatgg aacgtcgaat tagaccatgg attaataaga 2401 aaatcataga atatataggt gaagaagaag ctacattagt tgattttgtt tgttctaagg 2461 ttatggctca tagttcaccc cagagcattt tagatgatgt tgccatggta cttgatgaag 2521 aagcagaagt ttttatagtc aaaatgtgga gattattgat atatgaaaca gaagccaaga 2581 aaattggtct tgtgaagttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 2641 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2701 agtcgacaat caacctctgg atta