Construct: ORF ccsbBroad304_05002
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018408.1_s304c1, BRDN0000406729
- Derived from:
- ccsbBroadEn_05002
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF563 (147837)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05002
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147837 | ZNF563 | zinc finger protein 563 | NM_145276.3 | 100% | 100% | |
| 2 | human | 147837 | ZNF563 | zinc finger protein 563 | XM_011527698.2 | 93.2% | 93.2% | 0_1ins96 |
| 3 | human | 147837 | ZNF563 | zinc finger protein 563 | XM_006722650.3 | 92.2% | 86.3% | 0_1ins50;80_81ins61 |
| 4 | human | 147837 | ZNF563 | zinc finger protein 563 | XM_011527699.2 | 92.2% | 86.3% | 0_1ins50;80_81ins61 |
| 5 | human | 147837 | ZNF563 | zinc finger protein 563 | XM_011527700.2 | 92.2% | 86.3% | 0_1ins50;80_81ins61 |
| 6 | human | 147837 | ZNF563 | zinc finger protein 563 | XM_006722651.3 | 88.2% | 88.2% | 189_190ins168 |
| 7 | human | 51710 | ZNF44 | zinc finger protein 44 | NM_016264.4 | 65.1% | 56.4% | (many diffs) |
| 8 | human | 147837 | ZNF563 | zinc finger protein 563 | XM_005259750.4 | 63.4% | 63.4% | 189_190ins522 |
| 9 | human | 79973 | ZNF442 | zinc finger protein 442 | XM_006722908.3 | 62.3% | 55.3% | (many diffs) |
| 10 | human | 90576 | ZNF799 | zinc finger protein 799 | NM_001080821.3 | 62.1% | 54.8% | (many diffs) |
| 11 | human | 79973 | ZNF442 | zinc finger protein 442 | NM_030824.3 | 61.5% | 54.6% | (many diffs) |
| 12 | human | 79973 | ZNF442 | zinc finger protein 442 | XM_017027318.2 | 61.5% | 54.6% | (many diffs) |
| 13 | human | 51710 | ZNF44 | zinc finger protein 44 | XM_011528062.3 | 61.3% | 53% | (many diffs) |
| 14 | human | 79973 | ZNF442 | zinc finger protein 442 | XM_017027316.1 | 60.6% | 53.9% | (many diffs) |
| 15 | human | 51710 | ZNF44 | zinc finger protein 44 | NM_001164276.2 | 60.4% | 52.2% | (many diffs) |
| 16 | human | 10224 | ZNF443 | zinc finger protein 443 | NM_005815.5 | 60% | 53.2% | (many diffs) |
| 17 | human | 79973 | ZNF442 | zinc finger protein 442 | XM_017027317.1 | 59.6% | 52.2% | (many diffs) |
| 18 | human | 90576 | ZNF799 | zinc finger protein 799 | NM_001322497.1 | 58% | 50.9% | (many diffs) |
| 19 | human | 90576 | ZNF799 | zinc finger protein 799 | NM_001322498.1 | 58% | 50.9% | (many diffs) |
| 20 | human | 79973 | ZNF442 | zinc finger protein 442 | NM_001363774.1 | 57.8% | 50.6% | (many diffs) |
| 21 | human | 51710 | ZNF44 | zinc finger protein 44 | NM_001353552.1 | 50% | 43.9% | (many diffs) |
| 22 | human | 51710 | ZNF44 | zinc finger protein 44 | NM_001353553.1 | 50% | 43.9% | (many diffs) |
| 23 | human | 147837 | ZNF563 | zinc finger protein 563 | NM_001363608.1 | 48.1% | 41.7% | 595_596ins118;687_688ins623 |
| 24 | human | 51710 | ZNF44 | zinc finger protein 44 | XR_002958321.1 | 12.7% | (many diffs) | |
| 25 | human | 51710 | ZNF44 | zinc finger protein 44 | XR_001753701.1 | 12.2% | (many diffs) | |
| 26 | human | 51710 | ZNF44 | zinc finger protein 44 | XR_001753702.1 | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1494
- ORF length:
- 1428
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgcagtggcc tttgaggatg tggctgtgaa cttcacccag gaggaatggg 121 ctttgctggg tccatcacag aagaatttat acagatatgt gatgcaagaa accatcagga 181 acctggactg tataagaatg atatgggaag aacagaatac tgaagatcag tacaaaaatc 241 ctaggagaaa tctaagatgt catatggtag agagattcag tgaaagtaaa gacagtagtc 301 agtgtggaga aacatttagc ctcattcgag atagtattgt gaacaacagc atttgtcctg 361 gagaagatcc atgtcaaagc gctgagtgtg aagaagtcat aatgggtcat ttatccctta 421 atagccacat cagagttgat tctggacaca aaccacatga gtatcaggaa tatggagaga 481 agccacatac acataaacaa cgtgggaaag ccttcagtta ccatcactcc tttcagtcgc 541 gtggaaggcc tcacactgga aagaaacgct atgagtgtaa ggaatgtgga aaaaccttca 601 gttctcgtag aaaccttcga agacacatgg tagtgcaagg tggaaataga ccttataaat 661 gtaagttgtg tgggaaagct tttttttggc ccagtttatt acgtatgcat gaaagaactc 721 acactggaga gaaaccgtat gaatgtaagc agtgttctaa agcctttcct ttttacagtt 781 cctatcgaag acatgagaga atgcacactg gggagaaacc gtatgaatgt aagcagtgtt 841 ctaaagcctt gcctgattcc agttcctata taagacatga aagaactcac actggagaga 901 aaccatatac atgtaaacag tgtgggaaag ccttcagtgt ttccagttcc cttcgaagac 961 atgaaaccac tcacagtgca gagaaaccct atgagtgtaa gcaatgcggg aaaacatttc 1021 atcatcttgg aagctttcag atacacatga aaaggcacac tggagatcga cctcataaat 1081 gtaagatatg tgggaaaggc tttgatcgtc ccagtttagt tcgatatcat gaacgaattc 1141 acactggaga gaaaccctat gaatgcaagc agtgtgggaa aacgttatct catagctcaa 1201 gctttcgaag acacatgata atgcacactg gaggtggacc tcataaatgc aagatatgtg 1261 ggaaagcttt tgtttatcct agtgtatgtc aaagacacga aaagtctcac agtggagaga 1321 aaccctatga atgcaagcag tgtgggaaag cgttatctca tagctcaagc tttcgaagac 1381 atatggtaat gcatacggga gatgggccga ataaatgcaa ggtatgtggg aaagcctttg 1441 tttatcccag tgtatgtcaa agacatgaaa agactcactg gagagaaaca atatgcccaa 1501 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1561 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta