Construct: ORF ccsbBroad304_05039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004857.1_s304c1, BRDN0000409576
- Derived from:
- ccsbBroadEn_05039
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENTHD1 (150350)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05039
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150350 | ENTHD1 | ENTH domain containing 1 | NM_152512.4 | 100% | 100% | |
| 2 | human | 150350 | ENTHD1 | ENTH domain containing 1 | XM_006724149.3 | 100% | 100% | |
| 3 | human | 150350 | ENTHD1 | ENTH domain containing 1 | XM_011529927.2 | 96.5% | 96.5% | 1218_1219ins63 |
| 4 | human | 150350 | ENTHD1 | ENTH domain containing 1 | XM_006724150.3 | 91.2% | 91.2% | 0_1ins159 |
| 5 | human | 150350 | ENTHD1 | ENTH domain containing 1 | XM_011529928.2 | 86.6% | 86.6% | 349_350ins243 |
| 6 | human | 150350 | ENTHD1 | ENTH domain containing 1 | XM_006724151.2 | 81.2% | 80.7% | (many diffs) |
| 7 | human | 150350 | ENTHD1 | ENTH domain containing 1 | XM_011529930.2 | 56% | 54.3% | (many diffs) |
| 8 | human | 150350 | ENTHD1 | ENTH domain containing 1 | XM_011529931.2 | 48% | 46.3% | (many diffs) |
| 9 | human | 150350 | ENTHD1 | ENTH domain containing 1 | XM_006724153.3 | 45.7% | 45.6% | 832_833insAT;834_835ins985 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1887
- ORF length:
- 1821
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gttcaggaga caagtgaaaa actttgtgaa aaattactca gatgctgaaa 121 taaaagtcag ggaagcaact tctaacgacc cttggggtcc ctctagttct ctgatgttag 181 atatcagtga cttgactttc aacacaattt ctctctcaga gattatgaat atgctgtggc 241 acagactcaa tgaccatggg aagaactggc gccacgtgta taaatccctt accctaatgg 301 attatctcat caagaatgga tcaaagaaag ttattcagca ttgcagagag gggttctgta 361 accttcaaac actaaaagat tttcagcaca tagatgaagc tggaaaagac caaggttatt 421 atatccggga aaaatctaag caagtcatca cattgctgat ggatgaacca ttgctgtgta 481 aagagaggga agtggcatgt cggactagac agcgtacctc ccactctata ttgttttcta 541 aaagacaact tggttcaagt aactcactga cagcgtgcac ttctgccccc acaccggata 601 tttctgcttc agagaagaag tataagcttc ctaagtttgg aaggttacat aataaaagaa 661 atgtgtgcaa ggcaggattg aaacaagagc attgccaaga tgttcatttg cctacagaaa 721 ctatgttgtc ccaggaaaca cttccactca agattcatgg ttggaaatca acagaggacc 781 tgatgacatt tttggatgat gatccagagc tgcctttact agcaacacct ccttccattg 841 tctctccaat cacttgcttg tcagaagcag aagaagtttg taatctttcg ggtgcagatg 901 ctgtgcctac tctctcagaa aatagtcctt ctgggcagag agatgtgagt ttggacaaga 961 gatcagatgg tatctttaca aatactgtca cagaaaacct cttggaaaca cctttagaaa 1021 agcaatcagc tgcagaaggt cttaaaacat tgacaatttt accagcatgt tggtcaagta 1081 aagaggagtt tatcagcccc gacttaaggg tatcaaagtc agattctact ttccataacc 1141 aggcctctgt agaaacactc tgtctctctc cctcattcaa aatatttgac cgagtgaagg 1201 agattgtaat caacaaggcc taccagaaac cagcacaatc cagcattcag atggatgata 1261 aaatcctcaa gacaaccaca cgggtttcaa ctgcttctga gggagcatct tccttttctc 1321 ctttatccat gtcatctcct gatttagcct ctccagagaa gtcagctcat ctcttatcac 1381 caattctggc cggaccttcc ttctggactc tgtcccatca acagttgtct tctacctcct 1441 ttaaagatga agataagaca gccaagctgc atcactcctt tgcttctagg ggcccagtgt 1501 cttctgatgt agaggaaaat gatagcctca atctactggg aattcttcca aataactctg 1561 attctgctaa aaagaatata agtcacattt ctagtagtca ctggggggag ttttccaccc 1621 aaaatgtaga ccagttcatc cctctgtcct gttctggttt tcagtcaacc aaagacttcc 1681 cccaggaacc tgaagcaaag aattccatta gtgttctttt aagggaggta aaacgtgcga 1741 tcgctagatt acatgaagat ctgagcacag tgatccaaga acttaatgtc atcaataaca 1801 tcttgatgag catgagtctg aatagttcac aaataagcca gtcttcccag gtcccccagt 1861 cttctgaggg gagctcagat cagatctacc caactttctt gtacaaagtg gttggtaagc 1921 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1981 ggcgcgttaa gtcgacaatc aacctctgga tta