Construct: ORF ccsbBroad304_05655
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006975.1_s304c1, BRDN0000404216
- Derived from:
- ccsbBroadEn_05655
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND10 (404636)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05655
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 404636 | DENND10 | DENN domain containing 10 | NM_207009.4 | 99.9% | 100% | 942G>A |
| 2 | human | 404636 | DENND10 | DENN domain containing 10 | NM_001303111.2 | 94.7% | 94.7% | (many diffs) |
| 3 | human | 404636 | DENND10 | DENN domain containing 10 | XM_017016261.1 | 94.7% | 94.7% | (many diffs) |
| 4 | human | 404636 | DENND10 | DENN domain containing 10 | XM_017016262.2 | 94.7% | 94.7% | (many diffs) |
| 5 | human | 404636 | DENND10 | DENN domain containing 10 | XM_017016263.1 | 94.7% | 94.7% | (many diffs) |
| 6 | human | 404636 | DENND10 | DENN domain containing 10 | XM_017016264.2 | 84.5% | 83.7% | 899_900insTATTGCTCTAAAAACAAG;903_904insAT;906_907ins145 |
| 7 | human | 404636 | DENND10 | DENN domain containing 10 | NM_001303112.2 | 79.4% | 77.3% | 0_1ins151;32_33ins68;723G>A |
| 8 | human | 404636 | DENND10 | DENN domain containing 10 | XM_006717849.4 | 68.8% | 68.9% | 0_1ins333;609G>A |
| 9 | human | 404636 | DENND10 | DENN domain containing 10 | XM_006717850.3 | 68.8% | 68.9% | 0_1ins333;609G>A |
| 10 | human | 404636 | DENND10 | DENN domain containing 10 | XM_006717851.3 | 68.8% | 68.9% | 0_1ins333;609G>A |
| 11 | human | 404636 | DENND10 | DENN domain containing 10 | XM_006717852.3 | 68.8% | 68.9% | 0_1ins333;609G>A |
| 12 | human | 404636 | DENND10 | DENN domain containing 10 | XM_017016265.1 | 68.8% | 68.9% | 0_1ins333;609G>A |
| 13 | human | 404636 | DENND10 | DENN domain containing 10 | XM_017016266.2 | 68.8% | 68.9% | 0_1ins333;609G>A |
| 14 | human | 404636 | DENND10 | DENN domain containing 10 | XM_017016267.1 | 68.8% | 68.9% | 0_1ins333;609G>A |
| 15 | human | 404636 | DENND10 | DENN domain containing 10 | NM_001303113.2 | 57.6% | 55.4% | 0_1ins304;26_27ins149;489G>A |
| 16 | human | 55855 | DENND10P1 | DENND10 pseudogene 1 | NR_027141.2 | 42.5% | (many diffs) | |
| 17 | human | 404636 | DENND10 | DENN domain containing 10 | NR_130122.2 | 39.2% | (many diffs) | |
| 18 | mouse | 67894 | Fam45a | family with sequence simila... | NM_026437.3 | 88.6% | 90.4% | (many diffs) |
| 19 | mouse | 67894 | Fam45a | family with sequence simila... | NM_001167829.1 | 87% | 88.7% | (many diffs) |
| 20 | mouse | 67894 | Fam45a | family with sequence simila... | XM_006527309.3 | 82.4% | 80.8% | (many diffs) |
| 21 | mouse | 67894 | Fam45a | family with sequence simila... | NM_001347464.1 | 58.3% | 59.9% | (many diffs) |
| 22 | mouse | 67894 | Fam45a | family with sequence simila... | XM_006527311.2 | 58.3% | 59.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcggccgag gtggcggaca ctcagctgat gcttggagtc gggctgatcg 121 aaaaggacac aaatggagaa gttctgtggg tgtggtgtta tccttccacg acagccacat 181 taaggaacct gctgctgaga aaatgctgcc ttacagatga aaacaaactt ctccatccct 241 ttgtctttgg tcagtacaga agaacatggt tttatatcac aacaattgaa gttccagatt 301 cttccatttt gaaaaaggtg actcattttt ctattgtcct gaccgccaaa gattttaacc 361 cagagaagta tgctgccttc actaggatat tgtgtagaat gtacctgaaa catgggagcc 421 cagttaaaat gatggagagt tatattgcag ttctcacaaa ggggatatgc cagagtgaag 481 aaaacggctc tttccttagt aaggattttg atgcccgaaa ggcctacctg gctggctcca 541 tcaaagacat tgtatctcag tttggaatgg aaactgttat cttacacaca gcactgatgc 601 taaagaaaag aattgtggtg tatcacccca agatagaagc ggtccaggag ttcaccagga 661 ctctgcctgc cctggtgtgg caccgacagg actggaccat ccttcactct tacgtgcacc 721 tcaacgccga tgagctggaa gccctgcaga tgtgcacagg ttacgtcgct ggatttgtag 781 acttggaggt gagcaacaga ccagacctct atgatgtgtt tgtgaatctg gcagagagtg 841 agattaccat tgctcccctt gcaaaagagg ccatggcaat gggcaaactg cacaaagaaa 901 tgggtcagct aattgttcag tctgcagaag atccagagaa atcagagagc cacgttatac 961 aggatattgc tctaaaaaca agagaaatct ttaccaacct agcaccattt tcagaagttt 1021 cggctgatgg agaaaagaga gtccttaatt tggaggcgct aaagcaaaaa cgatttccac 1081 cagcaacaga aaacttcctt tatcatctag cagcagccga acaaatgctg aaaatctgcc 1141 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1201 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1261 tta