Construct: ORF ccsbBroad304_05724
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001969.1_s304c1, BRDN0000405057
- Derived from:
- ccsbBroadEn_05724
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ANXA8 (653145)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05724
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653145 | ANXA8 | annexin A8 | NM_001040084.3 | 100% | 100% | |
| 2 | human | 728113 | ANXA8L1 | annexin A8 like 1 | NM_001098845.3 | 99.4% | 99.3% | (many diffs) |
| 3 | human | 653145 | ANXA8 | annexin A8 | XM_006717951.3 | 93% | 92.8% | 1_72del;88G>T |
| 4 | human | 653145 | ANXA8 | annexin A8 | NM_001271702.1 | 89.5% | 89.5% | 112_225del |
| 5 | human | 728113 | ANXA8L1 | annexin A8 like 1 | NM_001278923.2 | 82% | 81.9% | (many diffs) |
| 6 | human | 653145 | ANXA8 | annexin A8 | NM_001271703.1 | 79.6% | 81% | (many diffs) |
| 7 | human | 653145 | ANXA8 | annexin A8 | XM_011540096.1 | 72.9% | 72.9% | 1_363del |
| 8 | human | 653145 | ANXA8 | annexin A8 | XM_011540098.1 | 65.8% | 65.8% | 1_363del;1008_1009ins96 |
| 9 | human | 728113 | ANXA8L1 | annexin A8 like 1 | NM_001278924.2 | 64.7% | 64.6% | (many diffs) |
| 10 | human | 653145 | ANXA8 | annexin A8 | XM_011540099.1 | 56.9% | 54.8% | (many diffs) |
| 11 | human | 653145 | ANXA8 | annexin A8 | XR_945805.1 | 53.4% | 1_378del;1360_1836del | |
| 12 | human | 653145 | ANXA8 | annexin A8 | XR_002957008.1 | 49.4% | 1_378del;1360_1982del | |
| 13 | human | 653145 | ANXA8 | annexin A8 | XR_246202.2 | 46.8% | 1_378del;1360_2094del | |
| 14 | human | 653145 | ANXA8 | annexin A8 | XR_945804.1 | 46.6% | 1_378del;1360_2104del | |
| 15 | human | 653145 | ANXA8 | annexin A8 | XR_428714.3 | 43% | 1_378del;788_789ins80;1280_2014del | |
| 16 | human | 653145 | ANXA8 | annexin A8 | XM_011540101.2 | 32.5% | 31% | (many diffs) |
| 17 | mouse | 11752 | Anxa8 | annexin A8 | NM_013473.4 | 88.7% | 92% | (many diffs) |
| 18 | mouse | 11752 | Anxa8 | annexin A8 | NM_001281845.1 | 81.9% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgggatggc ctggtggaaa tcctggattg aacaggaggg tgtcacagtg aagagcagct 121 cccacttcaa cccagaccct gatgcagaga ccctctacaa agccatgaag gggatcggga 181 ccaacgagca ggctatcatc gatgtgctca ccaagagaag caacacgcag cggcagcaga 241 tcgccaagtc cttcaaggct cagttcggca aggacctcac tgagaccttg aagtctgagc 301 tcagtggcaa gtttgagagg ctcattgtgg cccttatgta cccgccatac agatacgaag 361 ccaaggagct gcatgacgcc atgaagggct taggaaccaa ggagggtgtc atcattgaga 421 tcctggcctc tcggaccaag aaccagctgc gggagataat gaaggcgtat gaggaagact 481 atgggtccag cctggaggag gacatccaag cagacacaag tggctacctg gagaggatcc 541 tggtgtgcct cctgcagggc agcagggatg atgtgagcag ctttgtggac ccaggactgg 601 ccctccaaga cgcacaggat ctgtatgcgg caggcgagaa gattcgtggg actgatgaga 661 tgaaattcat caccatcctg tgcacgcgca gtgccactca cctgctgaga gtgtttgaag 721 agtatgagaa aattgccaac aagagcattg aggacagcat caagagtgag acccatggct 781 cactggagga ggccatgctc actgtggtga aatgcaccca aaacctccac agctactttg 841 cagagagact ctactatgcc atgaagggag cagggacgcg tgatgggacc ctgataagaa 901 acatcgtttc aaggagcgag attgacttaa atcttatcaa atgtcacttc aagaagatgt 961 acggcaagac cctcagcagc atgatcatgg aagacaccag cggtgactac aagaacgccc 1021 tgctgagcct ggtgggcagc gacccctgag gaactttctt gtacaaagtg gttggtaagc 1081 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1141 ggcgcgttaa gtcgacaatc aacctctgga tta