Construct: ORF ccsbBroad304_06291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004708.1_s304c1, BRDN0000410062
- Derived from:
- ccsbBroadEn_06291
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNAI1 (2770)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06291
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2770 | GNAI1 | G protein subunit alpha i1 | NM_002069.6 | 99.7% | 99.4% | 655A>G;819C>T;863C>A |
| 2 | human | 2770 | GNAI1 | G protein subunit alpha i1 | NM_001256414.2 | 85% | 84.7% | (many diffs) |
| 3 | human | 2773 | GNAI3 | G protein subunit alpha i3 | NM_006496.4 | 80.9% | 93.2% | (many diffs) |
| 4 | mouse | 14677 | Gnai1 | guanine nucleotide binding ... | NM_010305.1 | 88.2% | 99.4% | (many diffs) |
| 5 | mouse | 14679 | Gnai3 | guanine nucleotide binding ... | NM_010306.3 | 80.4% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcacgctg agcgccgagg acaaggcggc ggtggagcgg agtaagatga 121 tcgaccgcaa cctccgtgag gacggcgaga aggcggcgcg cgaggtcaag ctgctgctgc 181 tcggtgctgg tgaatctggt aaaagtacaa ttgtgaagca gatgaaaatt atccatgaag 241 ctggttattc agaagaggag tgtaaacaat acaaagcagt ggtctacagt aacaccatcc 301 agtcaattat tgctatcatt agggctatgg ggaggttgaa gatagacttt ggtgactcag 361 cccgggcgga tgatgcacgc caactctttg tgctagctgg agctgctgaa gaaggcttta 421 tgactgcaga acttgctgga gttataaaga gattgtggaa agatagtggt gtacaagcct 481 gtttcaacag atcccgagag taccagctta atgattctgc agcatactat ttgaatgact 541 tggacagaat agctcaacca aattacatcc cgactcaaca agatgttctc agaactagag 601 tgaaaactac aggaattgtt gaaacccatt ttactttcaa agatcttcat tttaaaatgt 661 ttgatgtggg aggtcagaga tctgagcgga agaagtggat tcattgcttc gaaggagtgg 721 cggcgatcat cttctgtgta gcactgagtg actacgacct ggttctagct gaagatgaag 781 aaatgaaccg aatgcatgaa agcatgaaat tgtttgacag catatgtaac aacaagtggt 841 ttacagatac atccattata ctttttctaa acaagaagga tctttttgaa gaaaaaatca 901 aaaagagccc tctcactata tgctatcaag aatatgcagg atcaaacaca tatgaagagg 961 cagctgcata tattcaatgt cagtttgaag acctcaataa aagaaaggac acaaaggaaa 1021 tatacaccca cttcacatgt gccacagata ctaagaatgt gcagtttgtt tttgatgctg 1081 taacagatgt catcataaaa aataatctaa aagattgtgg tctcttttac ccaactttct 1141 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1201 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta