Construct: ORF ccsbBroad304_06297

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006011.1_s304c1, BRDN0000406550
Derived from:
ccsbBroadEn_06297
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GNB2 (2783)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06297
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2783 GNB2 G protein subunit beta 2 NM_005273.4 99.9% 100% 954C>T
2 human 2782 GNB1 G protein subunit beta 1 NM_001282539.1 78.6% 90.2% (many diffs)
3 human 2782 GNB1 G protein subunit beta 1 NM_002074.5 78.6% 90.2% (many diffs)
4 human 2782 GNB1 G protein subunit beta 1 XM_017001059.2 78.6% 90.2% (many diffs)
5 human 2782 GNB1 G protein subunit beta 1 XM_017001060.2 78.6% 90.2% (many diffs)
6 human 2782 GNB1 G protein subunit beta 1 XM_017001061.2 78.6% 90.2% (many diffs)
7 human 2782 GNB1 G protein subunit beta 1 XM_024446495.1 78.6% 90.2% (many diffs)
8 human 59345 GNB4 G protein subunit beta 4 NM_021629.4 71.7% 90% (many diffs)
9 mouse 14693 Gnb2 guanine nucleotide binding ... NM_010312.4 92.7% 100% (many diffs)
10 mouse 14693 Gnb2 guanine nucleotide binding ... XM_006504553.3 92.7% 100% (many diffs)
11 mouse 14688 Gnb1 guanine nucleotide binding ... XM_017319977.2 77.1% 90.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1086
ORF length:
1020
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag tgagctggag caactgagac aggaggccga gcagctccgg aaccagatcc
121gggatgcccg aaaagcatgt ggggactcaa cactgaccca gatcacagct gggctggacc
181cagtggggag aatccagatg aggacccgga ggaccctccg tgggcacctg gcaaagatct
241atgccatgca ctgggggacc gactcaaggc tgctggtcag cgcctcccag gatgggaagc
301tcatcatctg ggacagctac accaccaaca aggtccacgc catcccgctg cgctcctcct
361gggtaatgac ctgtgcctac gcgccctcag ggaactttgt ggcctgtggg gggttggaca
421acatctgctc catctacagc ctcaagaccc gcgagggcaa cgtcagggtc agccgggagc
481tgcctggcca cactgggtac ctgtcgtgtt gccgcttcct ggatgacaac caaatcatca
541ccagctctgg ggataccacc tgtgccctgt gggacattga gacaggccag cagacagtgg
601gttttgctgg acacagtggg gatgtgatgt ccctgtccct ggcccccgat ggccgcacgt
661ttgtgtcagg cgcctgtgat gcctctatca agctgtggga cgtgcgggat tccatgtgcc
721gacagacctt catcggccat gaatccgaca tcaatgcagt ggctttcttc cccaacggct
781acgccttcac caccggctct gacgacgcca cgtgccgcct cttcgacctg cgggccgatc
841aggagctcct catgtactcc catgacaaca tcatctgtgg catcacctct gttgccttct
901cgcgcagcgg acggctgctg ctcgctggct acgacgactt caactgcaac atctgggatg
961ccatgaaggg cgaccgtgca ggagtcctcg ctggccacga caaccgcgtg agctgccttg
1021gggtcaccga cgatggcatg gctgtggcca cgggctcctg ggactccttc ctcaagatct
1081ggaactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1141gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1201acctctggat ta

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