Transcript: Human NM_001282539.1

Homo sapiens G protein subunit beta 1 (GNB1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
GNB1 (2782)
Length:
3151
CDS:
345..1367

Additional Resources:

NCBI RefSeq record:
NM_001282539.1
NBCI Gene record:
GNB1 (2782)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001282539.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430937 ATCCTATCCAACCGTACTAAC pLKO_005 1438 3UTR 100% 10.800 15.120 N GNB1 n/a
2 TRCN0000036779 CGAGCAACTTAAGAACCAGAT pLKO.1 377 CDS 100% 4.050 3.240 N GNB1 n/a
3 TRCN0000421587 GAAGGTAAACTTGAGTGTAAT pLKO_005 1673 3UTR 100% 13.200 9.240 N GNB1 n/a
4 TRCN0000034429 GCTGAAACCAAGAGCACAATT pLKO.1 1522 3UTR 100% 13.200 9.240 N Gnb1 n/a
5 TRCN0000301615 GCTGAAACCAAGAGCACAATT pLKO_005 1522 3UTR 100% 13.200 9.240 N Gnb1 n/a
6 TRCN0000429592 ACTCTCTCTCAGATCACAAAC pLKO_005 429 CDS 100% 10.800 7.560 N GNB1 n/a
7 TRCN0000036782 GCAGGATGGTAAACTTATCAT pLKO.1 566 CDS 100% 5.625 3.938 N GNB1 n/a
8 TRCN0000036781 CTGGATAACATTTGCTCCATT pLKO.1 693 CDS 100% 4.950 3.465 N GNB1 n/a
9 TRCN0000036783 GCTTGTGATGCTTCAGCCAAA pLKO.1 951 CDS 100% 4.050 2.835 N GNB1 n/a
10 TRCN0000036780 GCCATTTGCTTCTTTCCAAAT pLKO.1 1035 CDS 100% 1.080 0.756 N GNB1 n/a
11 TRCN0000109292 CGACGACTTCAACTGCAACAT pLKO.1 1211 CDS 100% 4.950 2.475 Y Gnb2 n/a
12 TRCN0000331994 CGACGACTTCAACTGCAACAT pLKO_005 1211 CDS 100% 4.950 2.475 Y Gnb2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001282539.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00654 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00654 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469902 TCTGGTCGCGCACGCAAGCGGTAA pLX_317 41.4% 100% 100% V5 n/a
4 ccsbBroadEn_06297 pDONR223 100% 78.6% 90.2% None (many diffs) n/a
5 ccsbBroad304_06297 pLX_304 0% 78.6% 90.2% V5 (many diffs) n/a
6 TRCN0000475025 GATTCCCGACGGCGCCTATTGAGT pLX_317 50.8% 78.6% 90.2% V5 (many diffs) n/a
7 ccsbBroadEn_00655 pDONR223 100% 78.6% 90.2% None (many diffs) n/a
8 ccsbBroad304_00655 pLX_304 0% 78.6% 90.2% V5 (many diffs) n/a
9 TRCN0000472058 GGGAACAATCACATTGATCACTTT pLX_317 45.7% 78.6% 90.2% V5 (many diffs) n/a
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