Construct: ORF ccsbBroad304_06386

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009936.1_s304c1, BRDN0000404135
Derived from:
ccsbBroadEn_06386
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HNMT (3176)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06386
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3176 HNMT histamine N-methyltransferase NM_006895.3 99.8% 99.6% 700A>G
2 human 3176 HNMT histamine N-methyltransferase XM_017003948.1 85.9% 84.2% (many diffs)
3 human 3176 HNMT histamine N-methyltransferase XM_011511064.2 56.7% 56.5% 0_1ins378;322A>G
4 human 3176 HNMT histamine N-methyltransferase XM_017003949.2 53.7% 48.3% (many diffs)
5 human 3176 HNMT histamine N-methyltransferase NM_001024075.2 34.8% 25.8% (many diffs)
6 human 3176 HNMT histamine N-methyltransferase XR_002959286.1 25% (many diffs)
7 human 3176 HNMT histamine N-methyltransferase NM_001024074.3 16.5% 15.7% (many diffs)
8 mouse 140483 Hnmt histamine N-methyltransferase NM_080462.2 86.2% 82.7% (many diffs)
9 mouse 140483 Hnmt histamine N-methyltransferase XM_006497671.3 86.2% 82.7% (many diffs)
10 mouse 140483 Hnmt histamine N-methyltransferase XM_006497670.3 82.8% 79.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
942
ORF length:
876
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc atcttccatg aggagcttgt tttctgacca cgggaaatat gttgaatctt
121tccggaggtt tctcaaccat tccacggaac accagtgcat gcaggaattc atggacaaga
181agctgccagg cataatagga aggattggag acacaaaatc agaaattaag attctaagca
241taggcggagg tgcaggtgaa attgatcttc aaattctctc caaagttcag gctcaatacc
301caggagtttg tatcaacaat gaagttgttg agccaagtgc tgaacaaatt gccaaataca
361aagagcttgt agccaagaca tcgaacctcg agaacgtaaa gtttgcttgg cataaggaga
421catcatctga ataccaaagt agaatgttgg agaaaaagga gcttcaaaag tgggacttta
481ttcatatgat tcaaatgctg tattatgtaa aagacatccc agctaccctg aaattcttcc
541atagtctctt aggtaccaat gctaagatgc tcattattgt tgtgtcagga agcagtggct
601gggacaagct gtggaaaaag tacggatcac gctttcccca ggatgacctc tgccagtata
661tcacatcaga tgacctcact cagatgctgg acaacctagg gcttaagtat gagtgctatg
721accttttgtc caccatggat atatctgact gctttattga tggtgatgaa aatggagacc
781tgctttggga ttttttgact gaaacctgca actttaatgc cacagcacca cctgatctca
841gagcagagct tgggaaagat ctacaagagc ctgaatttag tgctaagaaa gaggggaagg
901ttctttttaa taatactctg agtttcatag tgattgaggc atacccaact ttcttgtaca
961aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1021ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)