Transcript: Human XR_002959286.1

PREDICTED: Homo sapiens histamine N-methyltransferase (HNMT), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HNMT (3176)
Length:
3490
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002959286.1
NBCI Gene record:
HNMT (3176)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002959286.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413319 AGTGATTGAGGCATAACTATC pLKO_005 1251 3UTR 100% 10.800 15.120 N HNMT n/a
2 TRCN0000036432 CGGGAAATATGTTGAATCTTT pLKO.1 133 3UTR 100% 5.625 4.500 N HNMT n/a
3 TRCN0000420988 ATCACAAACTCATCCATTAAT pLKO_005 1340 3UTR 100% 15.000 10.500 N HNMT n/a
4 TRCN0000434933 CAATGCTAAGATGCTCATTAT pLKO_005 879 3UTR 100% 13.200 9.240 N HNMT n/a
5 TRCN0000416406 CTGGACAACCTAGGGCTTAAG pLKO_005 1009 3UTR 100% 10.800 7.560 N HNMT n/a
6 TRCN0000036433 GACTGCTTTATTGATGGTAAT pLKO.1 1069 3UTR 100% 10.800 7.560 N HNMT n/a
7 TRCN0000036430 GCCTGAATTTAGTGCTAAGAA pLKO.1 1191 3UTR 100% 5.625 3.938 N HNMT n/a
8 TRCN0000036431 CCCAGGAGTTTGTATCAACAA pLKO.1 621 3UTR 100% 4.950 3.465 N HNMT n/a
9 TRCN0000138391 CGCCTGTAATCCTAGCACTTT pLKO.1 286 3UTR 100% 4.950 2.475 Y DENND6A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002959286.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06386 pDONR223 100% 25% None (many diffs) n/a
2 ccsbBroad304_06386 pLX_304 0% 25% V5 (many diffs) n/a
3 TRCN0000473124 TTGAGCATGCCAGGCCTTCGGCCT pLX_317 46.6% 25% V5 (many diffs) n/a
4 ccsbBroadEn_15448 pDONR223 0% 4.3% None 1_97del;251_3490del n/a
5 ccsbBroad304_15448 pLX_304 0% 4.3% V5 1_97del;251_3490del n/a
6 TRCN0000473500 ATTCCCCAGCCCCATTGAGTCACT pLX_317 100% 4.3% V5 1_97del;251_3490del n/a
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