Construct: ORF ccsbBroad304_06702

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003657.1_s304c1, BRDN0000401901
Derived from:
ccsbBroadEn_06702
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDE3B (5140)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06702
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5140 PDE3B phosphodiesterase 3B NM_000922.4 99.8% 99.9% (many diffs)
2 human 5140 PDE3B phosphodiesterase 3B XM_017017911.2 96.4% 96.4% (many diffs)
3 human 5140 PDE3B phosphodiesterase 3B NM_001363569.1 95.2% 95.3% (many diffs)
4 human 5140 PDE3B phosphodiesterase 3B NM_001363570.1 93.3% 93.3% (many diffs)
5 human 5140 PDE3B phosphodiesterase 3B XM_017017912.1 86.4% 86.5% (many diffs)
6 human 5140 PDE3B phosphodiesterase 3B XM_006718249.3 65.9% 66% (many diffs)
7 human 5140 PDE3B phosphodiesterase 3B XR_001747903.2 54.1% (many diffs)
8 mouse 18576 Pde3b phosphodiesterase 3B, cGMP-... NM_011055.2 83% 80.1% (many diffs)
9 mouse 18576 Pde3b phosphodiesterase 3B, cGMP-... XM_006507438.3 82% 79.2% (many diffs)
10 mouse 18576 Pde3b phosphodiesterase 3B, cGMP-... XR_001785491.1 34.6% (many diffs)
11 mouse 18576 Pde3b phosphodiesterase 3B, cGMP-... XR_001785490.1 32.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3405
ORF length:
3336
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaggagggac gagcgagacg ccaaagccat gcggtccctg cagccgccgg
121atggggccgg ctcgcccccc gagagtctga ggaacggcta cgtgaagagc tgcgtgagcc
181ccttgcggca ggaccctccg cgcggcttct tcttccacct ctgccacttc tgcaacgtgg
241agctgcggcc gccgccggcc tctccccagc agccgcggcg ctgctccccc ttctgccggg
301cgcgcctctc gctgggcgcc ctggctgcct ttgtcctcgc cctgctgctg ggcgcggaac
361ccgagagctg ggctgccggg gccgcctggc tgcggacgct gctgagcgtg tgttcgcaca
421gcttgagccc cctcttcagc atcgcctgtg ccttcttctt cctcacctgc ttcctcaccc
481ggaccaagcg gggacccggc ccgggccgga gctgcggctc ctggtggctg ctggcgctgc
541ccgcctgctg ttacctgggg gacttcttgg tgtggcagtg gtggtcttgg ccttgggggg
601atggcgacgc agggtccgcg gccccgcaca cgcccccgga ggcggcagcg ggcaggttgc
661tgctggtgct gagctgcgta gggctgctgc tgacgctcgc gcacccgctg cggctgcggc
721actgcgttct ggtgctgctc ctggccagct tcgtctggtg ggtctccttc accagcctcg
781ggtcgctgcc ctccgccctc aggccgctgc tctccggcct ggtggggggc gctggctgcc
841tgctggccct ggggttggat cacttctttc aaatcaggga agcgcctctt catcctcgac
901tgtccagtgc cgccgaagaa aaagtgcctg tgatccgacc ccggaggagg tccagctgcg
961tgtcgttagg agaaactgca gccagttact atggcagttg caaaatattc aggagaccgt
1021cgttgccttg tatttccaga gaacagatga ttctttggga ttgggactta aaacaatggt
1081ataagcctca ttatcaaaat tctggaggtg gaaatggagt tgatctttca gtgctaaatg
1141aggctcgcaa tatggtgtca gatcttctga ctgatccaag ccttccacca caagtcattt
1201cctctctacg gagtattagt agcttaatgg gtgctttctc aggttcctgt aggccaaaga
1261ttaatcctct cacaccattt cctggatttt acccctgttc tgaaatagag gacccagctg
1321agaaagggga tagaaaactt aacaagggac taaataggaa tagtttgcca actccacagc
1381tgaggagaag ctcgggaact tcaggattgc tacctgttga acagtcttca aggtgggatc
1441gtaataatgg caaaaggcct caccaagaat ttggcatttc aagtcaagga tgctatctaa
1501atgggccttt taattcaaat ctactgacta tcccgaagca aaggtcatct tctgtatcac
1561tgactcacca tgtaggtctc agaagagctg gtgttttgtc cagtctgagt cctgtgaatt
1621cttccaacca tggaccagtg tctactggct ctctaactaa tcgatcaccc atagaatttc
1681ctgatactgc tgattttctt aataagccaa gcgttatctt gcagagatct ctgggcaatg
1741cacctaatac tccagatttt tatcagcaac ttagaaattc tgatagcaat ctgtgtaaca
1801gctgtggaca tcaaatgctg aaatatgttt caacatctga atcagatggt acagattgct
1861gcagtggaaa atcaggtgaa gaagaaaaca ttttctcgaa agaatcattc aaacttatgg
1921aaactcaaca agaagaggaa acagagaaga aagacagcag aaaattattt caggaaggtg
1981ataagtggct aacagaagag gcacagagtg aacagcaaac aaatattgaa caggaagtat
2041cactggacct gattttagta gaagagtatg actcattaat agaaaagatg agcaactgga
2101attttccaat ttttgaactt gtagaaaaga tgggagagaa atcaggaagg attctcagtc
2161aggttatgta taccttattt caagacactg gtttattgga aatatttaaa attcccactc
2221aacaatttat gaactatttt cgtgcattag aaaatggcta tcgagacatt ccttatcaca
2281atcgtataca tgccacagat gtgctacatg cagtttggta tctgacaaca cggccagttc
2341ctggcttaca gcagatccac aatggttgtg gaacaggaaa tgaaacagat tctgatggta
2401gaattaacca tgggcgaatt gcttatattt cttcgaagag ctgctctaat cctgatgaga
2461gttatggctg cctgtcttca aacattcctg cattagaatt gatggctcta tacgtggcag
2521ctgccatgca tgattatgat cacccaggga ggacaaatgc atttctagtg gctacaaatg
2581cccctcaggc agttttatac aatgacagat ctgttctgga aaatcatcat gctgcgtcag
2641cttggaatct atatctttct cgcccagaat acaacttcct tcttcatctt gatcatgtgg
2701aattcaagcg ctttcgtttt ttagtcattg aagcaatcct tgctacggat cttaaaaagc
2761attttgattt tctcgcagaa ttcaatgcca aggcaaatga tgtaaatagt aatggcatag
2821aatggagtaa tgaaaatgat cgcctcttgg tatgccaggt gtgcatcaaa ctggcagata
2881taaatggccc agcaaaagtt cgagacttgc atttgaaatg gacagaaggc attgtcaatg
2941aattttatga gcagggagat gaagaagcaa atcttggtct gcccatcagt ccattcatgg
3001atcgttcttc tcctcaacta gcaaaactcc aagaatcttt tatcacccac atagtgggtc
3061ccctgtgtaa ctcctatgat gctgctggtt tgctaccagg tcagtggtta gaagcagaag
3121aggataatga tactgaaagt ggtgatgatg aagacggtga agaattagat acagaagatg
3181aagaaatgga aaacaatcta aatccaaaac caccaagaag gaaaagcaga cggcgaatat
3241tttgtcagct aatgcaccac ctcactgaaa accacaagat atggaaggaa atcgtagagg
3301aagaagaaaa atgtaaagct gatgggaata aactgcaggt ggagaattcc tccttacctc
3361aagcagatga gattcaggta attgaagagg cagatgaaga ggaattgcca actttcttgt
3421acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
3481gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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