Construct: ORF ccsbBroad304_06840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004756.1_s304c1, BRDN0000408694
- Derived from:
- ccsbBroadEn_06840
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAP1GDS1 (5910)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06840
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100426.2 | 99.9% | 99.8% | 713A>G |
2 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_021159.5 | 99.7% | 99.6% | 713A>G;1301_1302insAGC |
3 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100427.2 | 99.7% | 99.5% | 3_4insGCA;710A>G |
4 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454164.1 | 99.6% | 99.3% | 3_4insGCA;710A>G;1298_1299insAGC |
5 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100428.2 | 91.8% | 91.7% | 364_365ins147;566A>G |
6 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454165.1 | 91.7% | 91.6% | 364_365ins147;566A>G;1154_1155insAGC |
7 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100429.2 | 91.7% | 91.4% | 3_4insGCA;361_362ins147;563A>G |
8 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454166.1 | 91.5% | 91.2% | (many diffs) |
9 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454167.1 | 86.6% | 86.5% | 0_1ins243;470A>G |
10 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454168.1 | 86.6% | 86.5% | 0_1ins243;470A>G |
11 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | NM_001100430.2 | 84.8% | 84.5% | 364_365ins276;437A>G |
12 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454169.1 | 78.5% | 78.4% | 0_1ins243;121_122ins147;323A>G |
13 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454170.1 | 78.5% | 78.4% | 0_1ins243;121_122ins147;323A>G |
14 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454171.1 | 78.5% | 78.4% | 0_1ins243;121_122ins147;323A>G |
15 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454172.1 | 78.3% | 78.2% | (many diffs) |
16 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454173.1 | 78.3% | 78.2% | (many diffs) |
17 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454174.1 | 70.5% | 70.3% | 0_1ins537;176A>G |
18 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454175.1 | 70.5% | 70.3% | 0_1ins537;176A>G |
19 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XM_024454176.1 | 70.5% | 70.3% | 0_1ins537;176A>G |
20 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XR_002959742.1 | 67.6% | (many diffs) | |
21 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XR_002959743.1 | 62% | (many diffs) | |
22 | human | 5910 | RAP1GDS1 | Rap1 GTPase-GDP dissociatio... | XR_002959744.1 | 61.9% | (many diffs) | |
23 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | NM_001040690.2 | 89% | 94.9% | (many diffs) |
24 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | NM_001286759.1 | 88.8% | 94.7% | (many diffs) |
25 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | NM_145544.3 | 81.3% | 87.5% | (many diffs) |
26 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | XM_017319566.1 | 81.1% | 87.3% | (many diffs) |
27 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | XM_006501428.3 | 62.2% | 68% | (many diffs) |
28 | mouse | 229877 | Rap1gds1 | RAP1, GTP-GDP dissociation ... | XM_006501429.3 | 62.2% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1893
- ORF length:
- 1824
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagataat ctcagtgata ccttgaagaa gctgaagata acagctgttg 121 acaagactga ggatagttta gaaggatgct tggattgtct gcttcaagcc ctggctcaaa 181 ataatacgga aacaagtgaa aaaatccaag caagtggaat acttcagctg tttgcaagtc 241 tgttgactcc acagtcttcc tgcaaagcca aagtagctaa catcatagca gaagtagcca 301 aaaatgagtt tatgcgaatt ccatgtgtgg atgctggatt gatttcacca ctggtgcagc 361 tgctaaatag caaagaccag gaagtgctgc ttcaaacggg cagggctcta ggaaacatat 421 gttacgatag ccatgagggc agaagtgcag ttgaccaagc aggtggtgca cagattgtaa 481 ttgaccattt aaggtcactg tgcagtataa cagatcccgc caatgagaag ctcttgactg 541 tcttttgtgg catgctgatg aactatagca atgagaatga ttcgcttcaa gctcagctta 601 tcaatatggg tgttattcct accttagtga aattactggg catccactgc caaaatgcag 661 ctcttacaga aatgtgtctt gttgcatttg gtaatttagc agaacttgag tcaagtaaag 721 aacagtttgc cagtacaaac attgctgaag agctagtaaa actcttcaag aaacaaatag 781 gacatgataa gagagaaatg atttttgaag ttcttgctcc attggcagaa aatgatgcta 841 ttaaactaca gctggttgaa gcaggcctag tagagtgtct actagagatt gttcagcaaa 901 aagtggatag tgacaaagaa gatgatatta ctgagctcaa aactggttca gatctcatgg 961 ttttattact tcttggagat gaatccatgc agaagttatt tgaaggagga aaaggtagtg 1021 tatttcaaag ggtactctct tggatcccat caaataacca ccagctacag cttgctggag 1081 cattggcaat tgcaaatttt gccagaaatg atgcaaattg tattcatatg gtagacaatg 1141 ggattgtaga aaaacttatg gatttactgg acagacatgt agaagatgga aatgtaacag 1201 tacagcatgc agcactaagt gccctcagaa acctggccat tccagttata aataaagcaa 1261 agatgttatc agctggggtc acagaggcag ttttgaaatt tcttaaatct gaaatgcctc 1321 ctgttcagtt caaacttctg ggaacattaa gaatgttaat agatgcacaa gcagaagctg 1381 ctgaacaatt gggaaagaat gttaagttag tggagcgttt ggtggaatgg tgtgaagcca 1441 aagatcatgc tggtgtgatg ggggagtcaa acagactgct gtctgccctt atacgacaca 1501 gtaaatcaaa agatgtaatt aaaaccattg tgcagagtgg tggcatcaag catctagtta 1561 ccatggcaac tagtgaacat gtaataatgc agaatgaagc tcttgttgct ttggcattaa 1621 tagcagcttt agaattgggc actgctgaga aagatctaga aagtgctaaa cttgtacaga 1681 ttttacatag actgctagca gatgagagaa gtgctcctga aatcaaatat aattccatgg 1741 tcctgatatg tgctcttatg ggatctgaat gtctacacaa ggaagtacag gatttggctt 1801 ttctagatgt cgtatccaaa cttcgcagtc atgagaacaa aagtgttgcc cagcaggcct 1861 ctctcacaga gcagagactt actgtggaaa gcttgccaac tttcttgtac aaagtggttg 1921 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1981 accggtggcg cgttaagtcg acaatcaacc tctggatta