Transcript: Mouse NM_145544.3

Mus musculus RAP1, GTP-GDP dissociation stimulator 1 (Rap1gds1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Rap1gds1 (229877)
Length:
3527
CDS:
163..1839

Additional Resources:

NCBI RefSeq record:
NM_145544.3
NBCI Gene record:
Rap1gds1 (229877)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_145544.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000105791 CCAGGAACGATGGAAATTGTA pLKO.1 1046 CDS 100% 5.625 4.500 N Rap1gds1 n/a
2 TRCN0000105790 CCTGCCTAATAAGATTTCTAA pLKO.1 1985 3UTR 100% 5.625 4.500 N Rap1gds1 n/a
3 TRCN0000029791 GCAGAACTTGAGTCAAGTAAA pLKO.1 643 CDS 100% 13.200 9.240 N RAP1GDS1 n/a
4 TRCN0000344335 GCAGAACTTGAGTCAAGTAAA pLKO_005 643 CDS 100% 13.200 9.240 N RAP1GDS1 n/a
5 TRCN0000105793 CTCAGAATAATGCAGAAACAA pLKO.1 266 CDS 100% 5.625 3.938 N Rap1gds1 n/a
6 TRCN0000105792 CGTTTGGTTGAATGGTGTGAA pLKO.1 1360 CDS 100% 4.950 3.465 N Rap1gds1 n/a
7 TRCN0000105794 CATGTAATCATGCAGAATGAA pLKO.1 1522 CDS 100% 5.625 3.375 N Rap1gds1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145544.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06840 pDONR223 100% 81.3% 87.5% None (many diffs) n/a
2 ccsbBroad304_06840 pLX_304 0% 81.3% 87.5% V5 (many diffs) n/a
3 TRCN0000472959 AGAGCGGCCACCCTCAACTTCTCT pLX_317 5.6% 81.3% 87.5% V5 (many diffs) n/a
4 ccsbBroadEn_15560 pDONR223 0% 81.1% 87.5% None (many diffs) n/a
5 ccsbBroad304_15560 pLX_304 0% 81.1% 87.5% V5 (many diffs) n/a
6 TRCN0000473828 ACCTTAGATAAAGTTGCTTTGGAC pLX_317 31.2% 81.1% 87.5% V5 (many diffs) n/a
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