Transcript: Human XM_024454166.1

PREDICTED: Homo sapiens Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RAP1GDS1 (5910)
Length:
3600
CDS:
186..1859

Additional Resources:

NCBI RefSeq record:
XM_024454166.1
NBCI Gene record:
RAP1GDS1 (5910)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024454166.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029790 CCCTTATACGACACAGTAAAT pLKO.1 1450 CDS 100% 13.200 18.480 N RAP1GDS1 n/a
2 TRCN0000344256 CCCTTATACGACACAGTAAAT pLKO_005 1450 CDS 100% 13.200 18.480 N RAP1GDS1 n/a
3 TRCN0000029793 GCTGAACAATTGGGAAAGAAT pLKO.1 1344 CDS 100% 5.625 3.938 N RAP1GDS1 n/a
4 TRCN0000344337 GCTGAACAATTGGGAAAGAAT pLKO_005 1344 CDS 100% 5.625 3.938 N RAP1GDS1 n/a
5 TRCN0000029792 GCTAACATCATAGCAGAAGTA pLKO.1 390 CDS 100% 4.950 3.465 N RAP1GDS1 n/a
6 TRCN0000344253 GCTAACATCATAGCAGAAGTA pLKO_005 390 CDS 100% 4.950 3.465 N RAP1GDS1 n/a
7 TRCN0000029789 GCCAGTACAAACATTGCTGAA pLKO.1 696 CDS 100% 4.050 2.835 N RAP1GDS1 n/a
8 TRCN0000344336 GCCAGTACAAACATTGCTGAA pLKO_005 696 CDS 100% 4.050 2.835 N RAP1GDS1 n/a
9 TRCN0000029791 GCAGAACTTGAGTCAAGTAAA pLKO.1 666 CDS 100% 13.200 7.920 N RAP1GDS1 n/a
10 TRCN0000344335 GCAGAACTTGAGTCAAGTAAA pLKO_005 666 CDS 100% 13.200 7.920 N RAP1GDS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024454166.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15560 pDONR223 0% 91.7% 91.4% None 3_4insGCA;167G>C;361_362ins147 n/a
2 ccsbBroad304_15560 pLX_304 0% 91.7% 91.4% V5 3_4insGCA;167G>C;361_362ins147 n/a
3 TRCN0000473828 ACCTTAGATAAAGTTGCTTTGGAC pLX_317 31.2% 91.7% 91.4% V5 3_4insGCA;167G>C;361_362ins147 n/a
4 ccsbBroadEn_06840 pDONR223 100% 91.5% 91.2% None (many diffs) n/a
5 ccsbBroad304_06840 pLX_304 0% 91.5% 91.2% V5 (many diffs) n/a
6 TRCN0000472959 AGAGCGGCCACCCTCAACTTCTCT pLX_317 5.6% 91.5% 91.2% V5 (many diffs) n/a
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