Transcript: Human NM_021159.5

Homo sapiens Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
RAP1GDS1 (5910)
Length:
3747
CDS:
183..2006

Additional Resources:

NCBI RefSeq record:
NM_021159.5
NBCI Gene record:
RAP1GDS1 (5910)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021159.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029790 CCCTTATACGACACAGTAAAT pLKO.1 1597 CDS 100% 13.200 18.480 N RAP1GDS1 n/a
2 TRCN0000344256 CCCTTATACGACACAGTAAAT pLKO_005 1597 CDS 100% 13.200 18.480 N RAP1GDS1 n/a
3 TRCN0000029793 GCTGAACAATTGGGAAAGAAT pLKO.1 1491 CDS 100% 5.625 3.938 N RAP1GDS1 n/a
4 TRCN0000344337 GCTGAACAATTGGGAAAGAAT pLKO_005 1491 CDS 100% 5.625 3.938 N RAP1GDS1 n/a
5 TRCN0000029792 GCTAACATCATAGCAGAAGTA pLKO.1 390 CDS 100% 4.950 3.465 N RAP1GDS1 n/a
6 TRCN0000344253 GCTAACATCATAGCAGAAGTA pLKO_005 390 CDS 100% 4.950 3.465 N RAP1GDS1 n/a
7 TRCN0000029789 GCCAGTACAAACATTGCTGAA pLKO.1 843 CDS 100% 4.050 2.835 N RAP1GDS1 n/a
8 TRCN0000344336 GCCAGTACAAACATTGCTGAA pLKO_005 843 CDS 100% 4.050 2.835 N RAP1GDS1 n/a
9 TRCN0000029791 GCAGAACTTGAGTCAAGTAAA pLKO.1 813 CDS 100% 13.200 7.920 N RAP1GDS1 n/a
10 TRCN0000344335 GCAGAACTTGAGTCAAGTAAA pLKO_005 813 CDS 100% 13.200 7.920 N RAP1GDS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021159.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15560 pDONR223 0% 99.9% 99.8% None 170G>C n/a
2 ccsbBroad304_15560 pLX_304 0% 99.9% 99.8% V5 170G>C n/a
3 TRCN0000473828 ACCTTAGATAAAGTTGCTTTGGAC pLX_317 31.2% 99.9% 99.8% V5 170G>C n/a
4 ccsbBroadEn_06840 pDONR223 100% 99.7% 99.6% None 713A>G;1301_1302insAGC n/a
5 ccsbBroad304_06840 pLX_304 0% 99.7% 99.6% V5 713A>G;1301_1302insAGC n/a
6 TRCN0000472959 AGAGCGGCCACCCTCAACTTCTCT pLX_317 5.6% 99.7% 99.6% V5 713A>G;1301_1302insAGC n/a
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