Construct: ORF ccsbBroad304_06859

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001538.1_s304c1, BRDN0000400299
Derived from:
ccsbBroadEn_06859
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RFX2 (5990)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06859
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5990 RFX2 regulatory factor X2 NM_000635.4 99.9% 99.7% 8A>N;1829G>A
2 human 5990 RFX2 regulatory factor X2 NM_134433.3 96.4% 96.2% 8A>N;522_523ins75;1754G>A
3 human 5990 RFX2 regulatory factor X2 XM_011528172.2 94.7% 94.4% (many diffs)
4 human 5990 RFX2 regulatory factor X2 XM_017027107.1 93.7% 93.6% 0_1ins135;1694G>A
5 human 5990 RFX2 regulatory factor X2 XR_001753732.2 73.6% (many diffs)
6 mouse 19725 Rfx2 regulatory factor X, 2 (inf... NM_027787.1 83.3% 87.5% (many diffs)
7 mouse 19725 Rfx2 regulatory factor X, 2 (inf... NM_009056.2 80.5% 84.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2235
ORF length:
2169
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca ganttccgag ggtggagcgg attcgccagc gtccgtggct ctgcgtccct
121cggcggcagc cccgcctgtg ccagcctccc cgcagagggt gttggtccag gcagccagct
181ccaatcccaa aggggcccag atgcagccga tctccctccc cagagttcag caggtacccc
241agcaggtgca gccggtgcag cacgtgtatc ctgcccaggt gcagtacgtg gaagggggag
301acgccgtcta caccaatgga gccatacgaa cagcctacac ctacaacccc gagcctcaga
361tgtacgcccc cagcagcacg gcttcttact tcgaggcccc aggcggtgcc caggtgaccg
421tggcagcctc gtccccgcca gcggtcccct cccacagcat ggtgggcatc accatggatg
481tcggggggag ccccatcgtc tccagcgcgg gagcctatct catccacggg gggatggaca
541gcaccagaca ctccctggcc cacacctccc gctcatcgcc cgccacgctt gaaatggcga
601ttgaaaacct ccaaaaaagc gaaggaatca catcacacaa aagcggttta ctcaacagcc
661atctccagtg gctgttggat aattatgaaa cagcggaagg tgtgagtctc cccagaagtt
721ctctttacaa ccactacctt cggcactgcc aggagcacaa gctagaccca gtgaacgccg
781cctccttcgg gaaactgatc cgttctgtgt ttatggggct gagaacgcgg cggctgggca
841ccaggggcaa ctcgaagtac cattactatg ggattcgtct gaagccggac tcaccactga
901accggctgca ggaggacacg cagtacatgg ccatgcggca gcagcccatg caccagaagc
961ccaggtaccg gccagcccag aagacggaca gcctcgggga cagcggctcc cacagcggcc
1021tgcacagcac tccggaacag accatggccg tgcagagcca gcaccaccag cagtacatag
1081atgtctccca cgtcttcccc gagttcccag cgcccgacct gggcagcttc ctgctgcagg
1141acggcgtcac actgcacgac gtcaaggccc tgcagctggt gtacagacgg cactgcgagg
1201caactgtaga tgtggtgatg aacctccagt tccactacat cgagaagctg tggctctcct
1261tctggaactc taaggcctcc tccagcgacg gccccacctc tcttcctgcc agtgacgaag
1321accccgaggg cgccgtcctg cccaaggaca agcttatctc cctgtgtcag tgcgacccca
1381tcctcaggtg gatgaggagc tgcgaccaca tcctctacca ggcgctggtg gagattctca
1441tccccgacgt gctgaggccg gtccccagta ccttgacaca ggccatccgt aactttgcca
1501agagcttgga aggctggttg acaaatgcca tgagtgactt cccacaacag gtcatccaga
1561ccaaggtggg cgtcgtcagt gccTTCGCCC AGACGCTGCG GCGCTACACG TCCCTCAACC
1621ACCTGGCGCA GGCGGCCCGG GCGGTGCTGC AGAACACGTC CCAGATCAAC CAGATGCTCA
1681GCGACCTCAA CCGCGTGGAC TTTGCCAACG TGCAGGAGCA GGCCTCGTGG GTGTGCCAGT
1741GCGAGGAGAG TGTGGTGCAG CGGCTGGAGC AGGATTTCAA GCTGACCCTG CAGCAGCAGA
1801GCTCCCTGGA CCAGTGGGCC AGCTGGCTGG ACAGTGTGGT CACCCAGGTC CTGAAGCAGC
1861ATGCCGGCAG CCCCAGCTTC CCCAAGGCCG CCCAGCAGTT CTTGCTGAAA TGGTCCTTTT
1921ACAGCTCCAT GGTGATCCGG GACCTGACCC TGCGCAGCGC TGCCAGCTTC GGCTCCTTCC
1981ACCTCATCCG CCTGCTCTAC GACGAGTACA TGTTCTACCT GGTGGAGCAC CGCGTCGCGG
2041AGGCCACCGG AGAGACGCCG ATCGCTGTGA TGGGAGAGTT CAACGATCTC GCCTCTCTGT
2101CGCTGACGCT GCTCGACAAA GATGACATGG GCGATGAGCA GCGTGGCAGC GAGGCGGGCC
2161CAGACGCCCG CAGCCTGGGT GAGCCCCTGG TAAAGCGGGA GCGCAGTGAC CCCAACCACT
2221CCCTGCAGGG CATCTACCCA ACTTTCTTGT ACaaagtggt tggtaagcct atccctaacc
2281ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2341cgacaatcaa cctctggatt a

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