Transcript: Mouse NM_027787.1

Mus musculus regulatory factor X, 2 (influences HLA class II expression) (Rfx2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Rfx2 (19725)
Length:
3353
CDS:
135..2288

Additional Resources:

NCBI RefSeq record:
NM_027787.1
NBCI Gene record:
Rfx2 (19725)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_027787.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000084556 CGCATCCTTTGGCAAACTCAT pLKO.1 833 CDS 100% 4.950 3.960 N Rfx2 n/a
2 TRCN0000084554 CAACTCAAAGTACCATTACTA pLKO.1 902 CDS 100% 5.625 3.938 N Rfx2 n/a
3 TRCN0000084557 CATCACCAGCAGTACATAGAT pLKO.1 1116 CDS 100% 5.625 3.938 N Rfx2 n/a
4 TRCN0000084555 GAAGCTCATCTCTCTGTGTAA pLKO.1 1403 CDS 100% 4.950 3.465 N Rfx2 n/a
5 TRCN0000084553 GCAACTAAGATGGTCACTCAA pLKO.1 2452 3UTR 100% 4.950 3.465 N Rfx2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_027787.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06859 pDONR223 100% 83.3% 87.5% None (many diffs) n/a
2 ccsbBroad304_06859 pLX_304 30.2% 83.3% 87.5% V5 (many diffs) n/a
3 TRCN0000491438 GGGTTAACCCAGCTTGCTCCGTTC pLX_317 11.3% 83.3% 87.5% V5 (many diffs) n/a
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