Construct: ORF ccsbBroad304_07008
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015268.1_s304c1, BRDN0000409315
- Derived from:
- ccsbBroadEn_07008
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAT5A (6776)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07008
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6776 | STAT5A | signal transducer and activ... | NM_001288718.1 | 99.9% | 100% | 1902C>T |
| 2 | human | 6776 | STAT5A | signal transducer and activ... | NM_003152.3 | 99.9% | 100% | 1902C>T |
| 3 | human | 6776 | STAT5A | signal transducer and activ... | NM_001288719.1 | 96.1% | 96.2% | 285_286ins90;1812C>T |
| 4 | human | 6776 | STAT5A | signal transducer and activ... | NM_001288720.1 | 96% | 96% | 1379_1380ins93;1809C>T |
| 5 | human | 6776 | STAT5A | signal transducer and activ... | XM_005257624.3 | 90.9% | 86.2% | 1902C>T;2062_2063ins160;2223_2283del |
| 6 | human | 6777 | STAT5B | signal transducer and activ... | NM_012448.4 | 87% | 91% | (many diffs) |
| 7 | human | 6777 | STAT5B | signal transducer and activ... | XM_024450897.1 | 87% | 91% | (many diffs) |
| 8 | human | 6777 | STAT5B | signal transducer and activ... | XM_024450898.1 | 87% | 91% | (many diffs) |
| 9 | human | 6777 | STAT5B | signal transducer and activ... | XM_017024977.1 | 77.2% | 80.7% | (many diffs) |
| 10 | human | 6777 | STAT5B | signal transducer and activ... | XM_005257626.4 | 56.5% | 58.8% | (many diffs) |
| 11 | mouse | 20850 | Stat5a | signal transducer and activ... | NM_011488.3 | 90.4% | 96.3% | (many diffs) |
| 12 | mouse | 20850 | Stat5a | signal transducer and activ... | XM_006532721.1 | 90.4% | 96.3% | (many diffs) |
| 13 | mouse | 20850 | Stat5a | signal transducer and activ... | XM_006532722.1 | 90.4% | 96.3% | (many diffs) |
| 14 | mouse | 20850 | Stat5a | signal transducer and activ... | XM_017314402.1 | 90.4% | 96.3% | (many diffs) |
| 15 | mouse | 20850 | Stat5a | signal transducer and activ... | NM_001164062.1 | 86.1% | 80.1% | (many diffs) |
| 16 | mouse | 20851 | Stat5b | signal transducer and activ... | NM_001113563.1 | 84.9% | 89.4% | (many diffs) |
| 17 | mouse | 20851 | Stat5b | signal transducer and activ... | NM_011489.3 | 84.9% | 89.4% | (many diffs) |
| 18 | mouse | 20851 | Stat5b | signal transducer and activ... | XM_017314403.1 | 84.9% | 89.4% | (many diffs) |
| 19 | mouse | 20851 | Stat5b | signal transducer and activ... | XM_006532723.2 | 83.2% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2448
- ORF length:
- 2382
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gggctggatc caggcccagc agctgcaggg agacgcgctg cgccagatgc 121 aggtgctgta cggccagcac ttccccatcg aggtccggca ctacttggcc cagtggattg 181 agagccagcc atgggatgcc attgacttgg acaatcccca ggacagagcc caagccaccc 241 agctcctgga gggcctggtg caggagctgc agaagaaggc ggagcaccag gtgggggaag 301 atgggttttt actgaagatc aagctggggc actacgccac gcagctccag aaaacatatg 361 accgctgccc cctggagctg gtccgctgca tccggcacat tctgtacaat gaacagaggc 421 tggtccgaga agccaacaat tgcagctctc cggctgggat cctggttgac gccatgtccc 481 agaagcacct tcagatcaac cagacatttg aggagctgcg actggtcacg caggacacag 541 agaatgagct gaagaaactg cagcagactc aggagtactt catcatccag taccaggaga 601 gcctgaggat ccaagctcag tttgcccagc tggcccagct gagcccccag gagcgtctga 661 gccgggagac ggccctccag cagaagcagg tgtctctgga ggcctggttg cagcgtgagg 721 cacagacact gcagcagtac cgcgtggagc tggccgagaa gcaccagaag accctgcagc 781 tgctgcggaa gcagcagacc atcatcctgg atgacgagct gatccagtgg aagcggcggc 841 agcagctggc cgggaacggc gggccccccg agggcagcct ggacgtgcta cagtcctggt 901 gtgagaagtt ggccgagatc atctggcaga accggcagca gatccgcagg gctgagcacc 961 tctgccagca gctgcccatc cccggcccag tggaggagat gctggccgag gtcaacgcca 1021 ccatcacgga cattatctca gccctggtga ccagcacatt catcattgag aagcagcctc 1081 ctcaggtcct gaagacccag accaagtttg cagccaccgt acgcctgctg gtgggcggga 1141 agctgaacgt gcacatgaat cccccccagg tgaaggccac catcatcagt gagcagcagg 1201 ccaagtctct gcttaaaaat gagaacaccc gcaacgagtg cagtggtgag atcctgaaca 1261 actgctgcgt gatggagtac caccaagcca cgggcaccct cagtgcccac ttcaggaaca 1321 tgtcactgaa gaggatcaag cgtgctgacc ggcggggtgc agagtccgtg acagaggaga 1381 agttcacagt cctgtttgag tctcagttca gtgttggcag caatgagctt gtgttccagg 1441 tgaagactct gtccctacct gtggttgtca tcgtccacgg cagccaggac cacaatgcca 1501 cggctactgt gctgtgggac aatgcctttg ctgagccggg cagggtgcca tttgccgtgc 1561 ctgacaaagt gctgtggccg cagctgtgtg aggcgctcaa catgaaattc aaggccgaag 1621 tgcagagcaa ccggggcctg accaaggaga acctcgtgtt cctggcgcag aaactgttca 1681 acaacagcag cagccacctg gaggactaca gtggcctgtc cgtgtcctgg tcccagttca 1741 acagggagaa cttgccgggc tggaactaca ccttctggca gtggtttgac ggggtgatgg 1801 aggtgttgaa gaagcaccac aagccccact ggaatgatgg ggccatccta ggttttgtga 1861 ataagcaaca ggcccacgac ctgctcatca acaagcccga cgggaccttc ttgttgcgct 1921 ttagtgactc agaaatcggg ggcatcacca tcgcctggaa gtttgattcc ccggaacgca 1981 acctgtggaa cctgaaacca ttcaccacgc gggatttctc catcaggtcc ctggctgacc 2041 ggctggggga cctgagctat ctcatctatg tgtttcctga ccgccccaag gatgaggtct 2101 tctccaagta ctacactcct gtgctggcta aagctgttga tggatatgtg aaaccacaga 2161 tcaagcaagt ggtccctgag tttgtgaatg catctgcaga tgctgggggc agcagcgcca 2221 cgtacatgga ccaggccccc tccccagctg tgtgccccca ggctccctat aacatgtacc 2281 cacagaaccc tgaccatgta ctcgatcagg atggagaatt cgacctggat gagaccatgg 2341 atgtggccag gcacgtggag gaactcttac gccgaccaat ggacagtctt gactcccgcc 2401 tctcgccccc tgccggtctt ttcacctctg ccagaggctc cctctcatgc ccaactttct 2461 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2521 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta