Construct: ORF ccsbBroad304_07090
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000622.1_s304c1, BRDN0000399832
- Derived from:
- ccsbBroadEn_07090
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPM1 (7168)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07090
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7168 | TPM1 | tropomyosin 1 | NM_000366.6 | 99.7% | 100% | 180C>T;453C>A |
| 2 | human | 7168 | TPM1 | tropomyosin 1 | XM_017022538.2 | 99.7% | 100% | 180C>T;453C>A |
| 3 | human | 7168 | TPM1 | tropomyosin 1 | XM_006720667.4 | 99.6% | 99.6% | 180C>T;453C>A;852A>G |
| 4 | human | 7168 | TPM1 | tropomyosin 1 | NM_001365779.1 | 96% | 94.3% | (many diffs) |
| 5 | human | 7168 | TPM1 | tropomyosin 1 | NM_001018005.2 | 95.9% | 94% | (many diffs) |
| 6 | human | 7168 | TPM1 | tropomyosin 1 | XM_017022539.2 | 94.6% | 93.6% | (many diffs) |
| 7 | human | 7168 | TPM1 | tropomyosin 1 | XM_005254639.4 | 94.2% | 90.6% | (many diffs) |
| 8 | human | 7168 | TPM1 | tropomyosin 1 | NM_001018006.2 | 93.1% | 92.2% | (many diffs) |
| 9 | human | 7168 | TPM1 | tropomyosin 1 | NM_001365776.1 | 91% | 88% | (many diffs) |
| 10 | human | 7168 | TPM1 | tropomyosin 1 | NM_001365777.1 | 90.6% | 85% | (many diffs) |
| 11 | human | 7168 | TPM1 | tropomyosin 1 | NM_001018004.2 | 89.5% | 86.6% | (many diffs) |
| 12 | human | 7168 | TPM1 | tropomyosin 1 | NM_001301244.2 | 86.6% | 84.5% | (many diffs) |
| 13 | human | 7168 | TPM1 | tropomyosin 1 | XM_017022536.2 | 86.6% | 78.9% | (many diffs) |
| 14 | human | 7168 | TPM1 | tropomyosin 1 | NM_001018020.2 | 84.1% | 82.7% | (many diffs) |
| 15 | human | 7168 | TPM1 | tropomyosin 1 | XM_017022534.2 | 83.4% | 74% | (many diffs) |
| 16 | human | 7168 | TPM1 | tropomyosin 1 | NM_001365781.2 | 81% | 75.8% | (many diffs) |
| 17 | human | 7168 | TPM1 | tropomyosin 1 | XM_005254650.3 | 80.9% | 75.5% | (many diffs) |
| 18 | human | 7168 | TPM1 | tropomyosin 1 | NM_001018007.2 | 80.7% | 77.1% | (many diffs) |
| 19 | human | 7168 | TPM1 | tropomyosin 1 | XM_017022537.2 | 78.4% | 70.7% | (many diffs) |
| 20 | human | 7168 | TPM1 | tropomyosin 1 | NM_001365778.1 | 78.3% | 75.4% | (many diffs) |
| 21 | human | 7168 | TPM1 | tropomyosin 1 | NM_001365782.1 | 77.5% | 70.3% | (many diffs) |
| 22 | human | 7168 | TPM1 | tropomyosin 1 | NM_001330346.2 | 77.4% | 70% | (many diffs) |
| 23 | human | 7168 | TPM1 | tropomyosin 1 | NM_001330351.2 | 76.1% | 69.6% | (many diffs) |
| 24 | human | 7168 | TPM1 | tropomyosin 1 | XM_017022535.2 | 75.4% | 65.8% | (many diffs) |
| 25 | human | 7168 | TPM1 | tropomyosin 1 | NM_001330344.2 | 74.9% | 68.2% | (many diffs) |
| 26 | human | 7168 | TPM1 | tropomyosin 1 | NM_001018008.2 | 72.7% | 64.1% | (many diffs) |
| 27 | human | 7168 | TPM1 | tropomyosin 1 | NM_001365780.1 | 72.6% | 61.5% | (many diffs) |
| 28 | human | 7168 | TPM1 | tropomyosin 1 | NM_001301289.2 | 71.6% | 62.7% | (many diffs) |
| 29 | human | 7168 | TPM1 | tropomyosin 1 | XM_005254646.2 | 69.9% | 58.3% | (many diffs) |
| 30 | human | 7168 | TPM1 | tropomyosin 1 | XM_017022540.1 | 66.9% | 53.5% | (many diffs) |
| 31 | human | 7168 | TPM1 | tropomyosin 1 | XM_024450040.1 | 46.8% | 40.5% | (many diffs) |
| 32 | human | 7168 | TPM1 | tropomyosin 1 | XR_002957675.1 | 26.6% | (many diffs) | |
| 33 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164251.1 | 94.7% | 99.2% | (many diffs) |
| 34 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164248.1 | 91% | 93.6% | (many diffs) |
| 35 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_011242715.2 | 89.5% | 90.2% | (many diffs) |
| 36 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164250.1 | 87.9% | 91.9% | (many diffs) |
| 37 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511043.3 | 87.3% | 89.4% | (many diffs) |
| 38 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511040.3 | 86% | 84.7% | (many diffs) |
| 39 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164255.1 | 85.3% | 87.6% | (many diffs) |
| 40 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_024427.4 | 84.4% | 86.2% | (many diffs) |
| 41 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511041.1 | 83.9% | 84.1% | (many diffs) |
| 42 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511042.3 | 83.8% | 83.8% | (many diffs) |
| 43 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_011242714.2 | 82.4% | 80.5% | (many diffs) |
| 44 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511046.2 | 81.6% | 83.4% | (many diffs) |
| 45 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511044.1 | 80.8% | 82% | (many diffs) |
| 46 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511050.2 | 78.5% | 75.5% | (many diffs) |
| 47 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511045.2 | 78.3% | 77.8% | (many diffs) |
| 48 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164249.1 | 77.5% | 76.4% | (many diffs) |
| 49 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164254.1 | 75% | 69.6% | (many diffs) |
| 50 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_011242716.2 | 73.6% | 66.6% | (many diffs) |
| 51 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511051.3 | 72.9% | 69.3% | (many diffs) |
| 52 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164253.1 | 72.1% | 67.9% | (many diffs) |
| 53 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164256.1 | 69.6% | 63.7% | (many diffs) |
| 54 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164252.1 | 68.9% | 62.4% | (many diffs) |
| 55 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_017313283.1 | 65.9% | 65.1% | (many diffs) |
| 56 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XR_379412.3 | 53.1% | (many diffs) | |
| 57 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XR_379413.1 | 37.6% | (many diffs) | |
| 58 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XR_001778851.1 | 24% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 918
- ORF length:
- 852
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgccatcaag aagaagatgc agatgctgaa gctcgacaag gagaacgcct 121 tggatcgagc tgagcaggcg gaggccgaca agaaggcggc ggaagacagg agcaagcagc 181 tggaagatga gctggtgtca ctgcaaaaga aactcaaggg caccgaagat gaactggaca 241 aatattctga ggctctcaaa gatgcccagg agaagctgga gctggcagag aaaaaggcca 301 ccgatgctga agccgacgta gcttctctga acagacgcat ccagctggtt gaggaagagt 361 tggatcgtgc ccaggagcgt ctggcaacag ctttgcagaa gctggaggaa gctgagaagg 421 cagcagatga gagtgagaga ggcatgaaag tcattgagag tcgagcccaa aaagatgaag 481 aaaaaatgga aattcaggag atccaactga aagaggcaaa gcacattgct gaagatgccg 541 accgcaaata tgaagaggtg gcccgtaagc tggtcatcat tgagagcgac ctggaacgtg 601 cagaggagcg ggctgagctc tcagaaggcc aagtccgaca gctggaagaa caattaagaa 661 taatggatca gaccttgaaa gcattaatgg ctgcagagga taagtactcg cagaaggaag 721 acagatatga ggaagagatc aaggtccttt ccgacaagct gaaggaggct gagactcggg 781 ctgagtttgc ggagaggtca gtaactaaat tggagaaaag cattgatgac ttagaagacg 841 agctgtacgc tcagaaactg aagtacaaag ccatcagcga ggagctggac cacgctctca 901 acgatatgac ttccatgtac ccaactttct tgtacaaagt ggttggtaag cctatcccta 961 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1021 agtcgacaat caacctctgg atta