Construct: ORF ccsbBroad304_07247
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001560.1_s304c1, BRDN0000461977
- Derived from:
- ccsbBroadEn_07247
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPFIA4 (8497)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07247
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XM_011510070.2 | 86.7% | 86.1% | (many diffs) |
| 2 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XM_017002609.2 | 60.2% | 60.2% | 1_1383del;2181C>A;2389C>A |
| 3 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | NM_001304331.2 | 58% | 57.9% | 1_1518del;2316C>A;2524C>A |
| 4 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | NM_001304332.2 | 57.2% | 57.2% | (many diffs) |
| 5 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XM_017002607.2 | 55% | 54.6% | (many diffs) |
| 6 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XM_006711586.2 | 53.1% | 52.7% | (many diffs) |
| 7 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XM_017002606.2 | 52.4% | 52.1% | (many diffs) |
| 8 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XR_001737497.2 | 47.2% | (many diffs) | |
| 9 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XR_426804.3 | 31.8% | (many diffs) | |
| 10 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XR_001737496.2 | 31.2% | (many diffs) | |
| 11 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XR_001737495.1 | 30.5% | (many diffs) | |
| 12 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XR_002957844.1 | 30.5% | (many diffs) | |
| 13 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XR_001737498.2 | 29.3% | (many diffs) | |
| 14 | human | 8497 | PPFIA4 | PTPRF interacting protein a... | XM_017002610.2 | 17.8% | 16.7% | (many diffs) |
| 15 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XM_006529847.3 | 53.2% | 56% | (many diffs) |
| 16 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XM_006529848.3 | 52.4% | 55.2% | (many diffs) |
| 17 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | NM_001144855.1 | 52.1% | 54.7% | (many diffs) |
| 18 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XR_387192.1 | 38.3% | (many diffs) | |
| 19 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XR_878325.2 | 35.5% | (many diffs) | |
| 20 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XM_006529849.3 | 33.5% | 35.1% | (many diffs) |
| 21 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XR_001785569.1 | 30.6% | (many diffs) | |
| 22 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XR_387189.3 | 30.4% | (many diffs) | |
| 23 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XR_387191.3 | 29.9% | (many diffs) | |
| 24 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XR_001785571.1 | 29.6% | (many diffs) | |
| 25 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XR_387190.2 | 29.6% | (many diffs) | |
| 26 | mouse | 68507 | Ppfia4 | protein tyrosine phosphatas... | XR_001785570.1 | 25.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2172
- ORF length:
- 2103
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcagtgca gcagacgtgc ggttctccct gggcacaacc acacacgcac 121 ccccaggcgt gcatcgccgc tactcggcat tgagggaaga gtctgccaag gactgggaga 181 cttctccact gcctgggatg ctggccccgg cagctggccc tgcctttgac agtgaccctg 241 agatctccga cgtggatgag gatgagccag ggggtctggt gggctctgcg gatgttgtct 301 cccccagcgg ccactcagat gcccagaccc tggccatgat gctgcaggag cagctggatg 361 ccatcaatga ggaaatcagg atgattcagg aagagaagga gtccacggag ctccgcgcgg 421 aggagattga gacgcgtgta accagtggca gcatggaagc cctaaacctg aagcagctgc 481 gcaagcgtgg ttccatcccc acctctctga cggccctgtc cctggccagc gcgtccccac 541 cactcagcgg ccgctccaca cctaagctca cctcccgcag tgctgcccag gacctggacc 601 gaatgggggt catgaccctg cccagtgact taagaaagca taggaggaag ctgctgtcgc 661 cagtgtctcg ggaagagaac cgagaggata aagccaccat aaaatgtgag acttctcctc 721 cttcctcacc caggacgctg cggctagaga agcttggcca cccagccctg agccaggaag 781 aaggcaagag tgccttggag gatcagggca gcaaccccag cagcagcaac agcagccagg 841 actccctgca caagggcgcc aagcgaaagg gcatcaagtc gtccattggc cgcctgtttg 901 ggaagaagga gaagggcagg ctgatccagc tgagtcggga tggagccaca ggccatgttc 961 tgctaacaga ctccgaattc agtatgcagg agcctatggt gcctgccaag ctggggaccc 1021 aggcagagaa ggaccggcgg ctaaagaaga aacaccagct gcttgaagat gccagcagga 1081 aaggaatgcc ctttgcccag tgggatggtc ctactgtggt ctcctggttg gagctctggg 1141 tggggatgcc tgcctggtat gtggcagcct gccgggccaa cgtcaagagt ggtgccatca 1201 tgtccgctct gtcggacaca gagatccagc gggagatcgg catcagcaat gccctgcacc 1261 ggctcaagct ccgcctggcc attcaggaga tggtgtcatt gaccagcccc tctgccccac 1321 ccacctccag gacttcttct gggaatgtct gggtcaccca tgaagagatg gaaactctgg 1381 aaacatctac taaaacagac agtgaggagg gcagctgggc tcagaccctg gcctatgggg 1441 acatgaacca tgagtggatt gggaatgaat ggctacccag cctggggctc ccgcagtacc 1501 gcagctactt catggagtgc ctggtggacg cccgcatgct ggaccacctc accaagaagg 1561 acctgcgggt ccacctgaag atggtggaca gcttccatcg aaccagtctt cagtatggca 1621 tcatgtgtct gaagaggctg aattatgacc ggaaggagct ggagaagagg cgagaggaga 1681 gccagcatga gatcaaggat gtgttagtct ggaccaacga ccaggtggtt cattgggtcc 1741 agtctattgg gctccgggac tacgcaggaa acctgcatga gagtggtgtg catggagcct 1801 tgctggccct ggacgagaac ttcgaccaca acacactggc cctgatcctc cagatcccca 1861 cacagaacac ccaggcacgc caagtgatgg aaagagagtt caataacctg ttggccttgg 1921 gcacagaccg gaagctggat gacggggatg acaaggtgtt tcgccgcgcg ccctcctgga 1981 ggaagcgctt ccggccgcgg gagcaccacg gtcgcggcgg catgctcagc gcttccgcgg 2041 agaccctccc ggcgggcttc cgtgtgtcca ccctggggac cctgcagccc ccaccggccc 2101 cgccaaagaa gatcatgcct gaagctcact cccactatct ctacggacac atgctctccg 2161 ccttccggga cttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2221 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2281 caatcaacct ctggatta