Construct: ORF ccsbBroad304_07395
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012327.1_s304c1, BRDN0000399230
- Derived from:
- ccsbBroadEn_07395
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFTUD2 (9343)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07395
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9343 | EFTUD2 | elongation factor Tu GTP bi... | NM_001258353.1 | 99.9% | 99.8% | 962G>T |
| 2 | human | 9343 | EFTUD2 | elongation factor Tu GTP bi... | NM_004247.4 | 99.9% | 99.8% | 962G>T |
| 3 | human | 9343 | EFTUD2 | elongation factor Tu GTP bi... | NM_001258354.1 | 98.9% | 98.8% | 424_425ins30;932G>T |
| 4 | human | 9343 | EFTUD2 | elongation factor Tu GTP bi... | NM_001142605.1 | 96.3% | 96.2% | 0_1ins105;857G>T |
| 5 | human | 9343 | EFTUD2 | elongation factor Tu GTP bi... | XR_934602.3 | 81.9% | (many diffs) | |
| 6 | mouse | 20624 | Eftud2 | elongation factor Tu GTP bi... | NM_001109995.1 | 91.5% | 99.2% | (many diffs) |
| 7 | mouse | 20624 | Eftud2 | elongation factor Tu GTP bi... | NM_011431.3 | 91.4% | 99.1% | (many diffs) |
| 8 | mouse | 20624 | Eftud2 | elongation factor Tu GTP bi... | XM_006532665.3 | 91.2% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2982
- ORF length:
- 2916
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga taccgactta tatgatgagt ttgggaatta tattggacca gagcttgatt 121 ctgatgaaga tgatgatgaa ttgggtagag agaccaaaga tcttgatgag atggatgatg 181 atgacgacga cgatgacgta ggagatcatg acgatgacca ccctgggatg gaggtggtgc 241 tgcatgagga caagaagtac tacccaacag ccgaggaggt gtatggtcct gaggtggaga 301 ccatagttca agaggaagac actcagcctc tcacagaacc cattattaag ccagtgaaaa 361 ccaagaaatt cactctgatg gagcagacat tacctgttac ggtgtatgag atggatttct 421 tggcggatct gatggataac tcagagctca tcagaaatgt gaccctttgt ggacatctcc 481 accatggcaa gacatgtttt gtggattgtt taattgaaca gactcacccg gaaatcagaa 541 agcgctatga ccaagatctg tgctatactg acatcctctt cacagagcaa gagagaggtg 601 taggcatcaa aagcactcct gtgacagtgg tcttgccaga caccaaagga aaatcttatc 661 tcttcaatat catggacact ccaggacatg tgaatttctc tgatgaggtc acagctggct 721 tgcgcatctc agatggagtg gtccttttca ttgatgctgc tgagggggtg atgctgaaca 781 cagagcggct gatcaagcat gcggtgcagg agaggctggc agtcactgtg tgcatcaaca 841 agattgaccg gctgatcctg gagctgaagc tgcctccaac tgatgcttat tacaagctgc 901 gccacattgt ggatgaggtc aatggattaa taagcatgta ttccactgat gagaacctga 961 tcctttcccc actcctgggt aacgtctgct tctccagctc ccagtacagc atctgcttca 1021 cgctggtctc ctttgccaag atctatgccg acacctttgg tgacattaat taccaagaat 1081 ttgctaaaag actctggggt gacatctact tcaaccctaa gacgcgaaag ttcaccaaaa 1141 aggccccaac tagcagctcc cagagaagtt tcgtggagtt tatcttggag cctctttata 1201 agatcctcgc ccaggttgta ggtgacgtgg acaccagcct cccacggacc ctagacgagc 1261 ttggcatcca cctgacgaag gaggagctga agctgaacat ccgccccttg ctcaggctgg 1321 tctgcaaaaa gttctttggc gagttcacag gctttgtgga catgtgtgtg cagcatatcc 1381 cttctccaaa ggtgggcgcc aagcccaaga ttgagcacac ctacaccggt ggtgtggact 1441 ccgacctcgg cgaggctatg agtgactgtg accctgatgg ccccctgatg tgccacacta 1501 ctaagatgta cagcacagat gatggagtcc agtttcacgc ctttggccgg gtgctgagtg 1561 gcaccattca tgctgggcag cctgtgaagg tactggggga gaactacacc ctggaggatg 1621 aggaagactc ccagatatgc accgtgggcc gcctttggat ctctgtggcc aggtaccaca 1681 tcgaggtgaa ccgtgttcct gctggcaact gggttctgat tgaaggtgtt gatcaaccaa 1741 ttgtgaagac agcaaccata accgaacccc gaggcaatga ggaggctcag attttccgac 1801 ccttgaagtt caataccaca tctgttatca agattgctgt ggagccagtc aacccctcag 1861 agctgcccaa gatgcttgat ggcctgcgca aggtcaacaa gagctatcca tccctcacca 1921 ccaaggtgga ggagtctggc gagcatgtga tcctgggcac tggggagctc tacctggact 1981 gtgtgatgca tgatttgcgg aagatgtact cagagataga catcaaggtg gctgacccag 2041 ttgtcacgtt ttgtgagacg gtggtggaaa catcctccct caagtgcttt gctgaaacgc 2101 ctaataagaa gaacaagatc accatgattg ctgagcctct tgagaagggc ctggcagagg 2161 acatagagaa tgaggtggtc cagattacgt ggaacaggaa gaagctggga gagttcttcc 2221 agaccaagta cgattgggat ctgctggctg cccgttccat ctgggctttt ggccctgatg 2281 cgactggccc caacattctg gtggatgata ctctgccctc tgaggtggac aaggctcttc 2341 ttggttcagt gaaggacagc atcgttcaag gtttccagtg gggaaccagg gagggccccc 2401 tctgtgatga attgattcgg aatgtcaagt ttaagatcct ggatgcggtg gttgcccagg 2461 agcccctgca ccggggcggg ggccagatca tccccacagc caggagagtc gtctactctg 2521 ccttcctcat ggctactcct cgtctgatgg agccttacta ctttgtagag gtccaggccc 2581 ctgcagattg cgtctctgca gtttataccg tcctggccag gcgcaggggg cacgtgactc 2641 aggatgcacc catcccaggc tcccctctgt acaccatcaa agcttttatc ccggccatcg 2701 actcttttgg ctttgagact gatctccgga ctcacaccca gggacaagcc ttttctctgt 2761 ctgtcttcca ccactggcag attgtgcctg gtgatcccct ggacaagagc attgtcatcc 2821 gccccttgga gccacagcca gctcctcacc tggcccggga attcatgatc aaaacccgcc 2881 gtaggaaggg cctcagtgaa gatgtgagca tcagcaaatt cttcgatgat cctatgttgc 2941 tggaacttgc caaacaggat gttgtgctca attaccccat gtgcccaact ttcttgtaca 3001 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 3061 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta