Construct: ORF ccsbBroad304_07462

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011665.1_s304c1, BRDN0000411437
Derived from:
ccsbBroadEn_07462
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDE4DIP (9659)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07462
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001002811.2 100% 100%
2 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001002812.2 79% 67.3% (many diffs)
3 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002901.2 50.1% 50.1% 3396_6771delinsA
4 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002900.2 49.7% 49.8% 3396_6819delinsA
5 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002897.2 47.8% 47.8% 3396_7098delinsA
6 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002896.2 47.8% 47.8% 3396_7101delinsA
7 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002890.2 47.4% 47.5% 3396_7149delinsA
8 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002886.2 47.1% 47.2% 3396_7194delinsA
9 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002885.2 47.1% 47.1% 3396_7197delinsA
10 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002884.2 46.5% 46.6% 3396_7287delinsA
11 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002883.2 46.2% 46.2% 3396_7335delinsA
12 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002882.2 46.2% 46.2% 3396_7338delinsA
13 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002881.2 46.2% 46.2% 3396_7341delinsA
14 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002879.2 45.9% 45.9% 3396_7389delinsA
15 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711648.3 45.5% 45.6% 3396_7446delinsA
16 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001350520.1 45.1% 45.1% 3396_7524delinsA
17 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_005272981.4 45.1% 45.1% 3396_7527delinsA
18 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510174.2 44.7% 44.7% 3396_7590delinsA
19 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002876.2 44.5% 44.5% 3396_7626delinsA
20 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002875.2 44.4% 44.4% 3396_7635delinsA
21 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_005277442.5 43.8% 43.8% 3396_7743delinsA
22 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711643.3 43.6% 43.6% 3396_7773delinsA
23 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711642.3 43.6% 43.6% 3396_7776delinsA
24 human 9659 PDE4DIP phosphodiesterase 4D intera... XR_001737551.2 42.2% 1_407del;3803_8036delinsA
25 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510176.2 39.5% 32.9% (many diffs)
26 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_024451068.1 39.3% 32.7% (many diffs)
27 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001198832.2 39.1% 32.7% (many diffs)
28 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002903.2 39.1% 32.7% (many diffs)
29 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001350523.1 38.9% 32.5% (many diffs)
30 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002899.2 38.2% 31.9% (many diffs)
31 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_024451067.1 37.9% 31.6% (many diffs)
32 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001350522.1 37.5% 31.4% (many diffs)
33 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001350521.2 37.2% 31% (many diffs)
34 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510175.2 36.9% 30.8% (many diffs)
35 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002878.2 36.9% 30.8% (many diffs)
36 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510179.1 35.9% 31% (many diffs)
37 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_014644.5 35.9% 31% (many diffs)
38 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510173.2 35.8% 29.9% (many diffs)
39 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_011510172.2 35.8% 29.8% (many diffs)
40 human 9659 PDE4DIP phosphodiesterase 4D intera... NM_001198834.3 35.6% 30.8% (many diffs)
41 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711646.2 35.4% 29.6% (many diffs)
42 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711652.1 34.7% 30.1% (many diffs)
43 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711651.1 34.7% 30.1% (many diffs)
44 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_006711650.4 34.3% 30.3% (many diffs)
45 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002887.1 34.1% 29.9% (many diffs)
46 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002888.1 34.1% 29.9% (many diffs)
47 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002889.2 34.1% 29.9% (many diffs)
48 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002891.2 33.1% 30.2% (many diffs)
49 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002892.2 33.1% 30.2% (many diffs)
50 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002893.1 33.1% 30.2% (many diffs)
51 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002894.2 33.1% 30.2% (many diffs)
52 human 9659 PDE4DIP phosphodiesterase 4D intera... XM_017002895.2 33.1% 30.2% (many diffs)
53 mouse 83679 Pde4dip phosphodiesterase 4D intera... NM_178080.4 84.3% 85.1% (many diffs)
54 mouse 83679 Pde4dip phosphodiesterase 4D intera... NR_110360.1 43% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3462
ORF length:
3396
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa ggagatttgc aggatctgtg cccgagagct gtgtggaaac cagcggcgct
121ggatcttcca cacggcgtcc aagctcaatc tccaggttct gctttcgcac gtcttgggca
181aggatgtccc ccgcgatggc aaagccgagt tcgcttgcag caagtgtgct ttcatgcttg
241atcgaatcta tcgattcgac acagttattg cccggattga agcgctttct attgagcgct
301tgcaaaagct gctactggag aaggatcgcc tcaagttctg cattgccagt atgtatcgga
361agaataacga tgactctggc gcggagatca aggcggggaa tgggacggtt gacatgtccg
421tcttacccga tgcgagatac tctgcactgc tccaggagga cttcgcctat tcagggtttg
481agtgctgggt ggagaatgag gatcagatcc aggagccaca cagctgccat ggttcagaag
541gccctggaaa ccgacccagg agatgccgtg gttgtgccgc tttgcgggtt gctgattctg
601actatgaagc catttgtaag gtacctcgaa aggtggccag aagtatctcc tgcggccctt
661ctagcaggtg gtcgaccagc atttgcactg aagaaccagc gttgtctgag gttgggccac
721ccgacttagc aagcacaaag gtacccccag atggagaaag catggaggaa gagacgcctg
781gttcctctgt ggaatctttg gatgcaagcg tccaggctag ccctccacaa cagaaagatg
841aggagactga gagaagtgca aaggaacttg gaaagtgtga ctgttgttca gatgatcagg
901ctccgcagca tgggtgtaat cacaagctgg aattagctct tagcatgatt aaaggtcttg
961attataagcc catccagagc ccccgaggga gcaggcttcc gattccagtg aaatccagcc
1021tacctggagc caagcctggc cctagcatga cagatggagt tagttccggt ttccttaaca
1081ggtctttgaa acccctttac aagacacctg tgagttatcc cttggagctt tcagacctgc
1141aggagctgtg ggatgatctc tgtgaagatt atttgccgct ccgggtccag cccatgactg
1201aagagttgct gaaacaacaa aagctgaatt cacatgagac cactataact cagcagtctg
1261tatctgattc ccacttggca gaactccagg aaaaaatcca gcaaacagag gccaccaaca
1321agattcttca agagaaactt aatgaaatga gctatgaact aaagtgtgct caggagtcgt
1381ctcaaaagca agatggtaca attcagaacc tcaaggaaac tctgaaaagc agggaacgtg
1441agactgagga gttgtaccag gtaattgaag gtcaaaatga cacaatggca aagcttcgag
1501aaatgctgca ccaaagccag cttggacaac ttcacagctc agagggtact tctccagctc
1561agcaacaggt agctctgctt gatcttcaga gtgctttatt ctgcagccaa cttgaaatac
1621agaagctcca gagggtggta cgacagaaag agcgccaact ggctgatgcc aaacaatgtg
1681tgcaatttgt agaggctgca gcacacgaga gtgaacagca gaaagaggct tcttggaaac
1741ataaccagga attgcgaaaa gccttgcagc agctacaaga agaattgcag aataagagcc
1801aacagcttcg tgcctgggag gctgaaaaat acaatgagat tcgaacccag gaacaaaaca
1861tccagcacct aaaccatagt ctgagtcaca aggagcagtt gcttcaggaa tttcgggagc
1921tcctacagta tcgagataac tcagacaaaa cccttgaagc aaatgaaatg ttgcttgaga
1981aacttcgcca gcgaatacat gataaagctg ttgctctgga gcgggctata gatgaaaaat
2041tctctgctct agaagagaaa gaaaaagaac tgcgccagct tcgtcttgct gtgagagagc
2101gagatcatga cttagagaga ctgcgcgatg tcctctcctc caatgaagct actatgcaaa
2161gtatggagag tctcctgagg gccaaaggcc tggaagtgga acagttatct actacctgtc
2221aaaacctcca gtggctgaaa gaagaaatgg aaaccaaatt tagccgttgg cagaaggaac
2281aagagagtat cattcagcag ttacagacgt ctcttcatga taggaacaaa gaagtggagg
2341atcttagtgc aacactgctc tgcaaacttg gaccagggca gagtgagata gcagaggagc
2401tgtgccagcg tctacagcga aaggaaagga tgctgcagga ccttctaagt gatcgaaata
2461aacaagtgct ggaacatgaa atggagattc aaggcctgct tcagtctgtg agcaccaggg
2521agcaggaaag ccaagctgct gcagagaagt tggtgcaagc cttaatggaa agaaattcag
2581aattacaggc cctgcgccaa tatttaggag ggagagactc cctgatgtcc caagcaccca
2641tctctaacca acaagctgaa gttaccccca ctggccgtct tggaaaacag actgatcaag
2701gttcaatgca gataccttcc agagatgata gcacttcatt gactgccaaa gaggatgtca
2761gcatacccag atccacatta ggagacttgg acacagttgc agggctggaa aaagaactga
2821gtaatgccaa agaggaactt gaactcatgg ctaaaaaaga aagagaaagt cagatggaac
2881tttctgctct acagtccatg atggctgtgc aggaagaaga gctgcaggtg caggctgctg
2941atatggagtc tctgaccagg aacatacaga ttaaagaaga tctcataaag gacctgcaaa
3001tgcaactggt tgatcctgaa gacataccag ctatggaacg cctgacccag gaagtcttac
3061ttcttcggga aaaagttgct tcagtagaat cccagggtca agaaatttca ggaaaccgaa
3121gacaacagtt gctgctgatg ctagaaggac tagtagatga acggagtcgg ctcaatgagg
3181ccttacaagc agagagacag ctctatagca gtctggtgaa gttccatgcc catccagaga
3241gctctgagag agaccgaact ctgcaggtgg aactggaagg ggctcaggtg ttacgcagtc
3301ggctagaaga agttcttgga agaagcttgg agcgcttaaa caggctggag accctggccg
3361ccattggagg tgcagctgca ggggatgaca ccgaagatac aagcactgag ttcactgaca
3421gtattgagga ggaggctgca caccatagtc accagcaact atacccaact ttcttgtaca
3481aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
3541ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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