Construct: ORF ccsbBroad304_07465

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008678.1_s304c1, BRDN0000410151
Derived from:
ccsbBroadEn_07465
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LRRC14 (9684)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07465
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9684 LRRC14 leucine rich repeat contain... NM_001272036.2 99.9% 100% 73T>C
2 human 9684 LRRC14 leucine rich repeat contain... NM_014665.4 99.9% 100% 73T>C
3 human 9684 LRRC14 leucine rich repeat contain... XM_005272358.5 99.9% 100% 73T>C
4 human 9684 LRRC14 leucine rich repeat contain... XM_005272359.5 99.9% 100% 73T>C
5 human 9684 LRRC14 leucine rich repeat contain... XM_024447336.1 99.9% 100% 73T>C
6 human 9684 LRRC14 leucine rich repeat contain... XM_005272360.5 92% 92% 0_1ins117
7 human 9684 LRRC14 leucine rich repeat contain... XM_017014005.2 92% 92% 0_1ins117
8 human 9684 LRRC14 leucine rich repeat contain... XM_024447337.1 92% 92% 0_1ins117
9 human 9684 LRRC14 leucine rich repeat contain... XM_024447338.1 76% 76% 0_1ins354
10 human 9684 LRRC14 leucine rich repeat contain... XM_024447339.1 76% 76% 0_1ins354
11 human 9684 LRRC14 leucine rich repeat contain... XM_024447340.1 76% 76% 0_1ins354
12 human 9684 LRRC14 leucine rich repeat contain... XM_024447341.1 76% 76% 0_1ins354
13 mouse 223664 Lrrc14 leucine rich repeat contain... NM_145471.2 85.4% 94.1% (many diffs)
14 mouse 223664 Lrrc14 leucine rich repeat contain... XM_006520785.3 85.4% 94.1% (many diffs)
15 mouse 223664 Lrrc14 leucine rich repeat contain... XM_006520786.3 85.4% 94.1% (many diffs)
16 mouse 223664 Lrrc14 leucine rich repeat contain... XM_006520787.2 85.4% 94.1% (many diffs)
17 mouse 223664 Lrrc14 leucine rich repeat contain... XM_006520789.3 85.4% 94.1% (many diffs)
18 mouse 223664 Lrrc14 leucine rich repeat contain... XM_011245579.2 85.4% 94.1% (many diffs)
19 mouse 223664 Lrrc14 leucine rich repeat contain... XM_017316558.1 85.4% 94.1% (many diffs)
20 mouse 223664 Lrrc14 leucine rich repeat contain... XM_017316559.1 85.4% 94.1% (many diffs)
21 mouse 223664 Lrrc14 leucine rich repeat contain... XM_017316560.1 52.2% 56.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1545
ORF length:
1479
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca cacgcttgtg ttcttgagca cacggcaggt gctgcagtgc cagccagctg
121cctgccaggc cctgcccctg ctgccacgcg aactcttccc cctgctgttc aaggtggcct
181tcatggacaa gaagacagtg gtactgcgcg agttggtaca cacgtggccc ttcccgctgc
241tcagtttcca gcagctgcta caggagtgtg cccactgcag ccgtgccctc ctgcaggagc
301ggcctagcac tgagagcatg caggctgtta tcctggggct gactgcccgg ctccacacct
361cagagcctgg ggccagcaca cagcccctct gcaggaagca tgcgctgcgg gtgctggaca
421tgacgggcct cttggatgat ggtgtggaac aggatcctgg caccatgagc atgtgggact
481gtactgctgc cgtagctcgc acatgcattg cccagcagca gggtggggcc gcagagcctg
541ggccagcccc catccccgtg gaggtgcgcg tggacctgcg ggtgaaccgg gcctcctatg
601cgttcctgcg ggaggcactc cgaagcagcg tgggcagccc gctgcggctc tgctgccggg
661acctgcgagc tgaggacctg cccatgcgca acactgtggc cctgctgcag cttctggatg
721caggctgcct gcgccgcgtg gacctgcgct tcaacaatct gggcctgcgc ggcctgtctg
781tgatcatccc acacgtggcc cgcttccagc acctggccag cctgcggctc cactatgtgc
841atggggattc aaggcagccc tccgtggatg gcgaggacaa cttccgctac ttccttgccc
901agatgggccg cttcacctgt ctgcgtgagc tcagcatggg ctcctctctc ctttcaggga
961ggctggacca gctgctcagc accctgcaga gccccctgga gagcctggag ttggccttct
1021gtgctctgct gcctgaggac ctacgcttcc tggcacggag cccacatgct gcccacctca
1081agaagttgga cctgagtggt aacgacctgt ctggcagcca gctggcaccc ttccagggtc
1141tgttgcaggc atcagcagcc acactgttgc atctggagct gactgagtgt cagctcgcag
1201acacccagct gttggccaca ctacccatcc tgactcagtg cgccagtctc cggtaccttg
1261gcctctatgg caacccactg tccatggcgg gcctcaagga gctgctgcgg gactcagtgg
1321cacaggctga gctgcgtact gtggtgcacc ccttccctgt ggactgctat gagggcttgc
1381cctggccgcc gcctgcctct gtcctgctgg aggcctccat caatgaggag aagtttgccc
1441gcgtagaagc tgagttgcac cagctgcttc tagcctcagg ccgtgcccat gtgctctgga
1501ccacggacat ctacgggcga ctggctgcgg actacttcag cctatgccca actttcttgt
1561acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1621gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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