Construct: ORF ccsbBroad304_08410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007037.1_s304c1, BRDN0000403948
- Derived from:
- ccsbBroadEn_08410
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYM (54808)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08410
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54808 | DYM | dymeclin | NM_017653.6 | 99.9% | 99.8% | 1222G>T |
| 2 | human | 54808 | DYM | dymeclin | NM_001353211.3 | 99.8% | 99.7% | 138_139insAGT;1219G>T |
| 3 | human | 54808 | DYM | dymeclin | NM_001353210.3 | 99.8% | 99.5% | 763_764insCAG;1219G>T |
| 4 | human | 54808 | DYM | dymeclin | NM_001374433.1 | 95% | 93% | (many diffs) |
| 5 | human | 54808 | DYM | dymeclin | XM_006722488.3 | 95% | 93% | (many diffs) |
| 6 | human | 54808 | DYM | dymeclin | NM_001374434.1 | 94.2% | 94.1% | 1222G>T;1745_1746ins114 |
| 7 | human | 54808 | DYM | dymeclin | NM_001374435.1 | 94.1% | 93.8% | 763_764insCAG;1219G>T;1742_1743ins114 |
| 8 | human | 54808 | DYM | dymeclin | XM_011526042.2 | 94% | 90% | (many diffs) |
| 9 | human | 54808 | DYM | dymeclin | NM_001374436.1 | 93.6% | 93.4% | 494_495ins126;1096G>T |
| 10 | human | 54808 | DYM | dymeclin | NM_001353214.3 | 92.3% | 92.2% | 1222G>T;1458_1622del |
| 11 | human | 54808 | DYM | dymeclin | NM_001374428.1 | 92.3% | 92.2% | 1222G>T;1458_1622del |
| 12 | human | 54808 | DYM | dymeclin | NM_001353212.3 | 92.2% | 92.1% | 138_139insAGT;1219G>T;1455_1619del |
| 13 | human | 54808 | DYM | dymeclin | NM_001353213.3 | 92.2% | 91.9% | 763_764insCAG;1219G>T;1455_1619del |
| 14 | human | 54808 | DYM | dymeclin | NM_001374429.1 | 92% | 91.8% | (many diffs) |
| 15 | human | 54808 | DYM | dymeclin | XM_011526039.2 | 91.9% | 88.5% | (many diffs) |
| 16 | human | 54808 | DYM | dymeclin | NM_001353216.3 | 90.8% | 90.7% | 1222G>T;1563_1564ins183 |
| 17 | human | 54808 | DYM | dymeclin | NM_001374437.1 | 90.8% | 90.5% | 763_764ins183;1039G>T |
| 18 | human | 54808 | DYM | dymeclin | NM_001374438.1 | 90.6% | 90.4% | 763_764insCAG;1219G>T;1560_1561ins183 |
| 19 | human | 54808 | DYM | dymeclin | XM_017025800.2 | 90.6% | 90.4% | 763_764insCAG;1219G>T;1560_1561ins183 |
| 20 | human | 54808 | DYM | dymeclin | NM_001374439.1 | 90.5% | 90.2% | (many diffs) |
| 21 | human | 54808 | DYM | dymeclin | XM_017025801.1 | 90.5% | 90.2% | (many diffs) |
| 22 | human | 54808 | DYM | dymeclin | XM_011526036.2 | 89.7% | 81.5% | 1222G>T;1859_1976del;2126_2235del |
| 23 | human | 54808 | DYM | dymeclin | XM_011526037.1 | 89.6% | 81.3% | (many diffs) |
| 24 | human | 54808 | DYM | dymeclin | XM_011526038.2 | 89.6% | 81.2% | (many diffs) |
| 25 | human | 54808 | DYM | dymeclin | XM_017025795.1 | 89.4% | 81.1% | (many diffs) |
| 26 | human | 54808 | DYM | dymeclin | NM_001374440.1 | 88.5% | 88.3% | 193_194ins228;994G>T |
| 27 | human | 54808 | DYM | dymeclin | NM_001374430.1 | 87.9% | 85.9% | (many diffs) |
| 28 | human | 54808 | DYM | dymeclin | NM_001374431.1 | 87.1% | 87% | 1222G>T;1458_1622del;1910_1911ins114 |
| 29 | human | 54808 | DYM | dymeclin | NM_001374432.1 | 86.5% | 86.3% | 494_495ins126;1096G>T;1332_1496del |
| 30 | human | 54808 | DYM | dymeclin | NM_001353215.3 | 83.9% | 83.8% | 1222G>T;1458_1622del;1728_1729ins183 |
| 31 | human | 54808 | DYM | dymeclin | XM_011526040.1 | 81.8% | 73.3% | (many diffs) |
| 32 | human | 54808 | DYM | dymeclin | XM_011526041.2 | 81.5% | 73.5% | (many diffs) |
| 33 | human | 54808 | DYM | dymeclin | XM_017025796.2 | 81.4% | 73.5% | (many diffs) |
| 34 | human | 54808 | DYM | dymeclin | XM_006722492.4 | 74.5% | 72.9% | (many diffs) |
| 35 | human | 54808 | DYM | dymeclin | XR_002958177.1 | 73.6% | (many diffs) | |
| 36 | human | 54808 | DYM | dymeclin | NM_001374442.1 | 71.5% | 71.3% | 193_194ins570;652G>T |
| 37 | human | 54808 | DYM | dymeclin | NM_001374443.1 | 71.4% | 71.3% | 191_192ins573;649G>T |
| 38 | human | 54808 | DYM | dymeclin | NM_001374441.1 | 66.1% | 65.8% | 193_194ins570;652G>T;888_1052del |
| 39 | human | 54808 | DYM | dymeclin | NM_001374444.1 | 62.4% | 62.1% | 193_194ins570;652G>T;993_994ins183 |
| 40 | mouse | 69190 | Dym | dymeclin | NM_027727.2 | 86.1% | 94.6% | (many diffs) |
| 41 | mouse | 69190 | Dym | dymeclin | XM_006526216.3 | 77.9% | 85.6% | (many diffs) |
| 42 | mouse | 69190 | Dym | dymeclin | XM_006526217.3 | 68% | 68.3% | (many diffs) |
| 43 | mouse | 69190 | Dym | dymeclin | XM_006526218.3 | 64.6% | 68.3% | (many diffs) |
| 44 | mouse | 69190 | Dym | dymeclin | XR_386014.3 | 64.4% | (many diffs) | |
| 45 | mouse | 69190 | Dym | dymeclin | XM_006526219.3 | 63.8% | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2073
- ORF length:
- 2007
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg atcgaatagc agcagaatcg gcgatcttcc taaaaatgag tacttgaaaa 121 agttatcagg cacggaatct atctctgaga atgacccgtt ctggaatcag cttctctcat 181 tttctttccc tgcaccaact agcagtagtg agttgaaact cttggaggaa gcaaccattt 241 cagtctgcag gtcattagtt gaaaacaatc ctcgaacagg aaatcttggt gcactaatta 301 aggtcttcct ttctagaacc aaagaactaa aactttcagc agaatgtcag aaccacatct 361 tcatttggca gacacacaat gctttgttta ttatttgctg tttgctgaaa gtgttcatct 421 gtcagatgtc agaggaggaa ttacaacttc attttactta tgaagaaaaa tctcctggca 481 attacagttc tgactcagaa gatcttttgg aagaattgct gtgctgtttg atgcagttga 541 tcactgatat tccactctta gatattacat atgaaatatc agtagaagct atatcaacaa 601 tggttgtttt cctttcctgc caactcttcc acaaagaagt tttgcgacag agcatcagcc 661 acaagtattt gatgcgaggt ccatgtcttc catacaccag caaacttgtg aagaccttat 721 tatataactt tatcagacaa gaaaagccac ctcctccagg ggcccatgtt ttccctcagc 781 agtcggatgg gggaggactg ctttatggac ttgcatcagg agtagcaaca ggactctgga 841 ctgtcttcac actaggtggt gtgggcagca aagcggctgc ctctccagag ctttcttccc 901 ctctggccaa ccagagtctc ctgcttctgc tggtgttggc caatctgaca gatgcctcag 961 atgcgccaaa cccctacaga caagccatta tgtccttcaa gaacacacaa gatagcagtc 1021 ctttcccctc atcaattcca catgccttcc agatcaactt taatagtttg tacacagctc 1081 tttgtgaaca gcagacatct gatcaagcaa ctctcctctt gtataccttg ctccatcaaa 1141 atagtaatat tagaacatac atgttggctc gcacagatat ggaaaatctt gttttaccaa 1201 ttcttgagat tctgtatcat gttgaagaaa ggaattcaca ccatgtgtat atggccctta 1261 taatattgtt gatccttacg gaagattatg gcttcaacag atccattcat gaagtgatac 1321 taaaaaatat tacttggtat tcagaacgag ttttaactga aatctccttg gggagtctcc 1381 tgatcctggt ggtaataaga accattcaat acaacatgac taggacacga gacaagtacc 1441 ttcacacaaa ttgtttggca gctttagcaa atatgtcggc acagtttcgt tctctccatc 1501 agtatgctgc ccagaggatc atcagtttat tttctttgct gtctaaaaaa cacaacaaag 1561 ttctggaaca agccacacag tccttgagag gttcgctgag ttctaatgat gttcctctac 1621 cagattatgc acaagaccta aatgtcattg aagaagtgat tcgaatgatg ttagagatca 1681 tcaactcctg cctgacaaat tcccttcacc acaacccaaa cttggtatac gccctgcttt 1741 acaaacgcga tctctttgaa caatttcgaa ctcatccttc atttcaggat ataatgcaaa 1801 atattgatct ggtgatctcc ttctttagct caaggttgct gcaagctgga gctgagctgt 1861 cagtggaacg ggtcctggaa atcattaagc aaggcgtcgt tgcgctgccc aaagacagac 1921 tgaagaaatt tccagaattg aaattcaaat atgtggaaga ggagcagccc gaggagtttt 1981 ttatccccta tgtctggtct cttgtctaca actcagcagt cggcctgtac tggaatccac 2041 aggacatcca gctgttcacc atggattccg actgcccaac tttcttgtac aaagtggttg 2101 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2161 accggtggcg cgttaagtcg acaatcaacc tctggatta