Construct: ORF ccsbBroad304_08464

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010397.1_s304c1, BRDN0000407658
Derived from:
ccsbBroadEn_08464
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WIPI1 (55062)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08464
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55062 WIPI1 WD repeat domain, phosphoin... NM_017983.7 99.9% 99.7% 92C>T
2 human 55062 WIPI1 WD repeat domain, phosphoin... NM_001320772.1 81.6% 81.6% 0_1ins246
3 human 55062 WIPI1 WD repeat domain, phosphoin... XM_017024808.1 71.3% 71.3% 0_1ins384
4 human 55062 WIPI1 WD repeat domain, phosphoin... NR_135471.1 65.3% (many diffs)
5 human 55062 WIPI1 WD repeat domain, phosphoin... NR_135470.1 64.5% (many diffs)
6 mouse 52639 Wipi1 WD repeat domain, phosphoin... NM_145940.2 87.5% 94.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1404
ORF length:
1338
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ggccgaggcc gcggacgctc ccccgggcgg ggttgagtcg gcgctcagct
121gcttctcttt caaccaggac tgcacatccc tagcaattgg aactaaagcc gggtataagc
181tgttttctct gagttctgtg gagcagctgg atcaagtcca cggaagcaat gaaatcccgg
241acgtctacat cgtggagcgc ctcttctcca gcagcctggt ggtggtagtc agtcacacaa
301aaccacggca gatgaacgtg tatcacttca agaaaggcac agagatctgt aattacagct
361actccagcaa catcttgtcc ataaggctga accggcaaag gctgctggtt tgcctagaag
421agtccattta tattcacaac attaaagaca tgaagctgtt gaagaccctc ctggatattc
481ctgcaaaccc aacaggtcta tgtgctctct ctatcaacca ttccaattct tacctggcct
541atcctggaag cctgacttca ggggagattg tgctttatga tggaaactcc ctgaaaacag
601tctgcactat tgctgcccat gagggaacac tagctgccat caccttcaat gcctcaggct
661ccaaactagc aagtgcgtct gaaaaaggca cagtcatccg ggtgttctct gtccctgatg
721ggcaaaagct ctatgagttc cggagaggga tgaaaaggta tgtgacaatc agctctctag
781tgttcagtat ggattcacaa ttcctctgcg cctccagtaa caccgagacg gtacacatct
841tcaagctgga acaggtcacc aacagtcgac cagaagagcc ttcgacctgg agtggctaca
901tgggaaagat gtttatggct gctaccaact acctccctac ccaggtgtca gacatgatgc
961atcaggacag ggcttttgcc actgcacgct tgaacttctc cggacagagg aacatctgta
1021ccctctcaac gatccagaag ttgccacggc tgctagttgc gtcatccagt ggacaccttt
1081atatgtacaa tttggatcct caggatggag gagagtgtgt cttaatcaaa acccacagct
1141tgcttggctc aggaacaaca gaagagaata aagaaaatga cctcagacct tccttacctc
1201agtcttatgc agcgaccgta gccagaccaa gtgcatcttc agcctccacg gtgccaggtt
1261attctgagga cggcggggcg ctgcgaggag aagttattcc tgaacatgag tttgcgacgg
1321gaccagtgtg tcttgatgat gagaatgagt ttcctcctat aatcttgtgc cgtggaaatc
1381agaagggcaa aacgaagcag tcatgcccaa ctttcttgta caaagtggtt ggtaagccta
1441tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1501gcgttaagtc gacaatcaac ctctggatta

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