Construct: ORF ccsbBroad304_08780
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007358.1_s304c1, BRDN0000401996
- Derived from:
- ccsbBroadEn_08780
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ARHGAP22 (58504)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08780
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NM_021226.4 | 99.6% | 38.6% | (many diffs) |
| 2 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NM_001256025.3 | 98.2% | 37% | (many diffs) |
| 3 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NM_001347738.2 | 98.2% | 37% | (many diffs) |
| 4 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NM_001256024.2 | 97.4% | 37.8% | (many diffs) |
| 5 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_011540002.2 | 96% | 36.1% | (many diffs) |
| 6 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_011540003.1 | 96% | 36.1% | (many diffs) |
| 7 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_024448099.1 | 96% | 36.1% | (many diffs) |
| 8 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NM_001347735.2 | 93.5% | 32.5% | (many diffs) |
| 9 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_011540006.2 | 92.2% | 30.9% | (many diffs) |
| 10 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NM_001256026.2 | 86.1% | 23.7% | (many diffs) |
| 11 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_005270014.3 | 84.3% | 23.2% | (many diffs) |
| 12 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_011540005.1 | 84.2% | 23.2% | (many diffs) |
| 13 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_011540011.2 | 80% | 17.9% | (many diffs) |
| 14 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_011540012.3 | 78.5% | 17.3% | (many diffs) |
| 15 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_017016471.2 | 78.5% | 17.3% | (many diffs) |
| 16 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NR_144645.2 | 74.5% | (many diffs) | |
| 17 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XR_002956998.1 | 70.1% | (many diffs) | |
| 18 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NR_144646.2 | 68.9% | (many diffs) | |
| 19 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NR_144643.2 | 65.3% | (many diffs) | |
| 20 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XR_002957004.1 | 65.2% | (many diffs) | |
| 21 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XR_001747166.2 | 65.2% | (many diffs) | |
| 22 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NR_144642.2 | 63.6% | (many diffs) | |
| 23 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NR_144644.2 | 61.2% | (many diffs) | |
| 24 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NR_045675.2 | 60.6% | (many diffs) | |
| 25 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XR_002956999.1 | 59.2% | (many diffs) | |
| 26 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_024448101.1 | 56.5% | 14.6% | (many diffs) |
| 27 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_017016476.1 | 54.7% | 65.2% | (many diffs) |
| 28 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_024448100.1 | 53.8% | 61.9% | (many diffs) |
| 29 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_011540013.3 | 52.6% | 59.7% | (many diffs) |
| 30 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_011540015.3 | 45.6% | 72.5% | (many diffs) |
| 31 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XR_002957000.1 | 42.2% | (many diffs) | |
| 32 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XR_002957003.1 | 39.3% | (many diffs) | |
| 33 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XR_002957002.1 | 38.5% | (many diffs) | |
| 34 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_024448102.1 | 16.8% | 36.6% | (many diffs) |
| 35 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_024448103.1 | 15.9% | 35.6% | (many diffs) |
| 36 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_024448104.1 | 15.8% | 36.2% | (many diffs) |
| 37 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_024448105.1 | 15.4% | 35.4% | (many diffs) |
| 38 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NM_001347737.1 | 11.5% | 28.7% | (many diffs) |
| 39 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | NM_001347736.1 | 11% | 24.8% | (many diffs) |
| 40 | human | 58504 | ARHGAP22 | Rho GTPase activating prote... | XM_024448106.1 | 11% | 24.8% | (many diffs) |
| 41 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | NM_153800.4 | 79.7% | 34.8% | (many diffs) |
| 42 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_011245053.2 | 79.7% | 34.8% | (many diffs) |
| 43 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_006518957.3 | 79.4% | 35.6% | (many diffs) |
| 44 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_017316004.1 | 78.1% | 33.4% | (many diffs) |
| 45 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_006518956.3 | 77.9% | 34% | (many diffs) |
| 46 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_006518954.3 | 76.4% | 32.7% | (many diffs) |
| 47 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_017316006.1 | 72.7% | 27.8% | (many diffs) |
| 48 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_006518961.3 | 69.9% | 22.8% | (many diffs) |
| 49 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_006518962.3 | 69.9% | 22.8% | (many diffs) |
| 50 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_006518959.2 | 68.4% | 22.3% | (many diffs) |
| 51 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_006518960.3 | 68.4% | 22.3% | (many diffs) |
| 52 | mouse | 239027 | Arhgap22 | Rho GTPase activating prote... | XM_017316007.1 | 40.3% | 62.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 882
- ORF length:
- 813
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgagccca aagatcaggc aggccaggag ggcccgctcc aaaagcctag 121 tgatggggga gcagagccgg agccctgggc ggatgccgtg ccctcacagg ctgggccccg 181 tgctgaaggc gggctggctg aagaagcaga ggagcatcat gaagaactgg cagcagcgct 241 ggtttgtgct gcgtggggat cagcttttct actacaagga caaagatgag atcaagcccc 301 agggatttat ttctctacaa gggacacagg tgactgaact tcctcctggc cccgaggacc 361 cagggaagca cctctttgag atcagcccag gtggtgctgg ggagcgggag aaggtgccgg 421 ccaaccccga ggcgctcctg ctcatggcca gctcccagcg tgacatggag gactgggtgc 481 aggccatccg ccgagtcatc tgggccccgc tgggcggagg gatctttggg cagcgcctag 541 aggaaacagt ccaccacgag cggaagtatg gcccccgcct ggcgcacctg ctggtggagc 601 agtgtgtgga cttcatccgg gagcgcgggc tcactgagga ggggctgttc cgcatgccgg 661 gccaggccaa cctggtgagg gacctgcagg attccttcga ctgtggggag aagccactgt 721 ttgacagcac aacagacgtg cacacggtgg cctccctgct gaagctgtac ctgcgggagc 781 tccccgagcc cgtggtcccc ttcgccaggt acgaggactt cctcagctgc gcccagctgc 841 tcaccaagga cgagggggag ggcactctgg agttggctaa ataagtgagc aaccttcctc 901 aggcaaatta caacctgctc agatacatct gcaagtttct ggatgaagtt caggcatact 961 caaatgtcaa caagatgagt gtccagaatc tggcaaccgt ttttggacct aacattctgc 1021 ggccacaggt agaggaccca gtaaccatca tggaaggcac ttccctcgtc cagcacctga 1081 tgaccgtcct catccgcaaa cacagccagc tcttcacggc accggtcccg gaagggccca 1141 cctccccgcg cgggggcctg caatgcgcag tggggtgggg ctccgaggag gtcaccaggg 1201 acagccaagg agagcccggc ggccccggcc tgcccgcgca caggacctct tccctggacg 1261 gggcggccgt ggcggtgctc tccagaacag cccccaaggg gccggggagc cggtgcagcc 1321 ctgggaagaa ggtgcagacc ctgcccagtt ggaagtcctc cttccggcag ccgaggtccc 1381 tatcgggaag cccgaagggg ggcggctcat ccctggaggt gcccatcatc tcctccggcg 1441 ggaactggct tatgaacggg ctgtcctccc tgcgcggaca ccgccgggcc tcgtcgggag 1501 accggctcaa ggactcgggc tccgtgcaga gactctccac ctacgacaat gtgcccgcgc 1561 cgggcctggt ccccggcata cccagcgtgg ccagtatggc gtggtccggg gcctcgtcca 1621 gcgagtcgtc ggtggggggc tcactcagca gctgcacggc ctgccgcgcc agcgactcgt 1681 ctgcccgcag ttccctgcac accgactggg ccctggagcc ctccccgctc cccagcagca 1741 gcgaggaccc caagtccctg gacctggacc acagcatgga cgaggcgggc gcgggtgcca 1801 gcaacagcga gccaagcgag ccggacagcc ccacccggga acacgcgcgc cgctccgagg 1861 ccttacaggg gctggtcact gagctcaggg ccgagctgtg ccgccagcgg actgagtacg 1921 agaggagtgt gaaaagaatc gaagaaggga gtgctgacct gagaaaacga atgtcccggt 1981 tagaagaaga actggaccag gaaaagaaaa aatacatcat gctggaaata aagctgcggg 2041 actctgaacg ggcgcgggag gatgcggaga ggaggaacca gctgttgcag agggaaatgg 2101 aggagttttt ttcgacccta ggaagcttga ctgttggggc aaaaggtgcc agggccccaa 2161 agttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2221 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2281 tctggatta