Transcript: Mouse XM_017316007.1

PREDICTED: Mus musculus Rho GTPase activating protein 22 (Arhgap22), transcript variant X13, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Arhgap22 (239027)
Length:
1374
CDS:
141..1289

Additional Resources:

NCBI RefSeq record:
XM_017316007.1
NBCI Gene record:
Arhgap22 (239027)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017316007.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197662 CCACTCAGATGTCAATAAGAT pLKO.1 1154 CDS 100% 5.625 7.875 N Arhgap22 n/a
2 TRCN0000181968 CCCACTCAGATGTCAATAAGA pLKO.1 1153 CDS 100% 5.625 3.938 N Arhgap22 n/a
3 TRCN0000047205 CAGGGATTTATTTCTCTACAA pLKO.1 450 CDS 100% 4.950 3.465 N ARHGAP22 n/a
4 TRCN0000047203 GCTCAGATACATCTGCAAGTT pLKO.1 1115 CDS 100% 4.950 3.465 N ARHGAP22 n/a
5 TRCN0000177132 GCTCTTCTACTACAAAGACAA pLKO.1 413 CDS 100% 4.950 3.465 N Arhgap22 n/a
6 TRCN0000182081 CCACAGATAGAGGATCCAGTA pLKO.1 1221 CDS 100% 4.050 2.835 N Arhgap22 n/a
7 TRCN0000182379 GCATCTGATGACTGTCCTCAT pLKO.1 1282 CDS 100% 4.050 2.835 N Arhgap22 n/a
8 TRCN0000181601 GCTAAACAAGTGAGCAACCTT pLKO.1 1074 CDS 100% 3.000 2.100 N Arhgap22 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017316007.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12416 pDONR223 100% 42.2% 41.4% None (many diffs) n/a
2 ccsbBroad304_12416 pLX_304 0% 42.2% 41.4% V5 (many diffs) n/a
3 TRCN0000478477 TTATCTGGAGTACACCTTAATACC pLX_317 16.2% 42.2% 41.4% V5 (many diffs) n/a
4 ccsbBroadEn_08780 pDONR223 100% 40.3% 62.7% None (many diffs) n/a
5 ccsbBroad304_08780 pLX_304 0% 40.3% 62.7% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000478424 GAGTTTAGGGGTTTCCTCCTTGGC pLX_317 13.6% 40.3% 62.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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