Transcript: Mouse XM_006518962.3

PREDICTED: Mus musculus Rho GTPase activating protein 22 (Arhgap22), transcript variant X10, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Arhgap22 (239027)
Length:
2322
CDS:
144..1964

Additional Resources:

NCBI RefSeq record:
XM_006518962.3
NBCI Gene record:
Arhgap22 (239027)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006518962.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197662 CCACTCAGATGTCAATAAGAT pLKO.1 761 CDS 100% 5.625 7.875 N Arhgap22 n/a
2 TRCN0000181968 CCCACTCAGATGTCAATAAGA pLKO.1 760 CDS 100% 5.625 3.938 N Arhgap22 n/a
3 TRCN0000047203 GCTCAGATACATCTGCAAGTT pLKO.1 722 CDS 100% 4.950 3.465 N ARHGAP22 n/a
4 TRCN0000182081 CCACAGATAGAGGATCCAGTA pLKO.1 828 CDS 100% 4.050 2.835 N Arhgap22 n/a
5 TRCN0000182379 GCATCTGATGACTGTCCTCAT pLKO.1 878 CDS 100% 4.050 2.835 N Arhgap22 n/a
6 TRCN0000181601 GCTAAACAAGTGAGCAACCTT pLKO.1 681 CDS 100% 3.000 2.100 N Arhgap22 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006518962.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08780 pDONR223 100% 69.9% 22.8% None (many diffs) n/a
2 ccsbBroad304_08780 pLX_304 0% 69.9% 22.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000478424 GAGTTTAGGGGTTTCCTCCTTGGC pLX_317 13.6% 69.9% 22.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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