Transcript: Human NM_025267.3

Homo sapiens PTGES3L-AARSD1 readthrough (PTGES3L-AARSD1), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
PTGES3L-AARSD1 (100885850)
Length:
1800
CDS:
146..1723

Additional Resources:

NCBI RefSeq record:
NM_025267.3
NBCI Gene record:
PTGES3L-AARSD1 (100885850)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_025267.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000147170 CAGTTGCTGACCATCTATTTA pLKO.1 834 CDS 100% 15.000 7.500 Y AARSD1 n/a
2 TRCN0000276177 CAGTTGCTGACCATCTATTTA pLKO_005 834 CDS 100% 15.000 7.500 Y PTGES3L-AARSD1 n/a
3 TRCN0000276112 AGCGCTCTTCCCGCTCTATTA pLKO_005 294 CDS 100% 13.200 6.600 Y PTGES3L-AARSD1 n/a
4 TRCN0000276176 CCGATGTCCACGTGCTTATTG pLKO_005 171 CDS 100% 13.200 6.600 Y PTGES3L-AARSD1 n/a
5 TRCN0000147352 GAACAGAACCAACCTGATATT pLKO.1 1183 CDS 100% 13.200 6.600 Y AARSD1 n/a
6 TRCN0000276113 GAACAGAACCAACCTGATATT pLKO_005 1183 CDS 100% 13.200 6.600 Y PTGES3L-AARSD1 n/a
7 TRCN0000179266 GCAGTTGCTGACCATCTATTT pLKO.1 833 CDS 100% 13.200 6.600 Y AARSD1 n/a
8 TRCN0000102508 GACCTTCAGGTCATTAAGATT pLKO.1 1139 CDS 100% 5.625 2.813 Y Aarsd1 n/a
9 TRCN0000327620 GACCTTCAGGTCATTAAGATT pLKO_005 1139 CDS 100% 5.625 2.813 Y Aarsd1 n/a
10 TRCN0000147937 GCAGAAGAATAACCTGAATCT pLKO.1 1339 CDS 100% 4.950 2.475 Y AARSD1 n/a
11 TRCN0000149557 GAAGACAACATCATGGGAGTT pLKO.1 859 CDS 100% 4.050 2.025 Y AARSD1 n/a
12 TRCN0000276174 GAAGACAACATCATGGGAGTT pLKO_005 859 CDS 100% 4.050 2.025 Y PTGES3L-AARSD1 n/a
13 TRCN0000183695 GAGATTGAGTTCTATGCCAAA pLKO.1 248 CDS 100% 4.050 2.025 Y AARSD1 n/a
14 TRCN0000178854 CCTGATATTTCTGTCTGGGAA pLKO.1 1195 CDS 100% 2.640 1.320 Y AARSD1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025267.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09044 pDONR223 100% 99.9% 100% None 399C>T n/a
2 ccsbBroad304_09044 pLX_304 0% 99.9% 100% V5 399C>T n/a
3 TRCN0000467834 ACAGACACAACCAACTTTGAGGAA pLX_317 28.9% 99.9% 100% V5 399C>T n/a
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