Construct: ORF ccsbBroad304_09113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016374.1_s304c1, BRDN0000462226
- Derived from:
- ccsbBroadEn_09113
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LONP2 (83752)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09113
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83752 | LONP2 | lon peptidase 2, peroxisomal | NM_031490.5 | 99.9% | 99.8% | 2548A>T |
| 2 | human | 83752 | LONP2 | lon peptidase 2, peroxisomal | NM_001300948.3 | 94.7% | 94.7% | 468_469ins132;2416A>T |
| 3 | human | 83752 | LONP2 | lon peptidase 2, peroxisomal | NM_001348078.2 | 93.9% | 93.9% | 2548A>T;2556_2718delinsG |
| 4 | human | 83752 | LONP2 | lon peptidase 2, peroxisomal | XM_017023755.2 | 75.9% | 75.8% | 1939_1941delATA;1943C>T;1947_1948ins612 |
| 5 | human | 83752 | LONP2 | lon peptidase 2, peroxisomal | XM_017023756.1 | 70.7% | 70.6% | (many diffs) |
| 6 | mouse | 66887 | Lonp2 | lon peptidase 2, peroxisomal | NM_025827.3 | 88.2% | 95.4% | (many diffs) |
| 7 | mouse | 66887 | Lonp2 | lon peptidase 2, peroxisomal | NM_001168591.1 | 44.5% | 47.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2625
- ORF length:
- 2556
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcatcagtg agccccatcc agatccccag tcgcctcccg ctgctgctca 121 cccacgaggg cgtcctgctg cccggctcca ccatgcgcac cagcgtggac tcggcccgca 181 acctgcagct ggtgcggagc cgccttctga agggcacgtc gctgcaaagc accatcctgg 241 gcgtcatccc caacacgcct gaccccgcca gcgacgcgca ggacctgccg ccgctgcaca 301 ggattggcac agctgcactg gccgttcagg ttgtgggcag taactggccc aagccccact 361 acactctgtt gattacaggc ctatgccgtt tccagattgt acaggtctta aaagagaagc 421 catatcccat tgctgaagtg gagcagttgg accgacttga ggagtttccc aacacctgta 481 aaatgaggga ggagctagga gaactatcag agcagtttta caaatatgca gtacaattgg 541 ttgaaatgtt ggatatgtct gtccctgcag ttgctaaatt gagacgtctt ttagatagtc 601 ttccaaggga agctttacca gacatcttga catcaattat ccgaacaagc aacaaagaga 661 aactccagat tttagatgct gtgagcctag aggagcggtt caagatgact ataccactgc 721 ttgtcagaca aattgaaggc ctgaaattgc ttcaaaaaac cagaaaaccc aagcaagatg 781 atgataagag ggttatagca atacgcccta ttaggagaat tacacatatc tcaggtactt 841 tagaagatga agatgaagat gaagataatg atgacattgt catgctagag aaaaaaatac 901 gaacatctag tatgccagag caggcccata aagtctgtgt caaagagata aagagactca 961 aaaaaatgcc tcagtcaatg ccagaatatg ctctgactag aaattatttg gaacttatgg 1021 tagaacttcc ttggaacaaa agtacaactg accgcctgga cattagggca gcccggattc 1081 ttctggataa tgaccattac gccatggaaa aattgaagaa aagagtactg gaatacttgg 1141 ctgtcagaca gctcaaaaat aacctgaagg gcccaatcct atgctttgtt ggccctcctg 1201 gagttggtaa aacaagtgtg ggaagatcag tggccaagac tctaggtcga gagttccaca 1261 ggattgcact tggaggagta tgtgatcagt ctgacattcg aggacacagg cgcacctatg 1321 ttggcagcat gcctggtcgc atcatcaacg gcttgaagac tgtgggagtg aacaacccag 1381 tgttcctatt agatgaggtt gacaaactgg gaaaaagtct acagggtgat ccagcagcag 1441 ctctgcttga ggtgttggat cctgaacaaa accataactt cacagatcat tatctaaatg 1501 tggcctttga cctttctcaa gttcttttta tagctactgc caacaccact gctaccattc 1561 cagctgcctt gttggacaga atggagatca ttcaggttcc aggttataca caggaggaga 1621 agatagagat tgcccatagg cacttgatcc ccaagcagct ggaacaacat gggctgactc 1681 cacagcagat tcagataccc caggtcacca ctcttgacat catcaccagg tataccagag 1741 aggcaggggt tcgttctctg gatagaaaac ttggggccat ttgccgagct gtggccgtga 1801 aggtggcaga aggacagcat aaggaagcca agttggaccg ttctgatgtg actgagagag 1861 aaggttgcag agaacacatc ttagaagatg aaaaacctga atctatcagt gacactactg 1921 acttggctct accacctgaa atgccgattt tgattgattt ccatgctctg aaagacatcc 1981 ttgggccccc gatgtatgaa atggaggtat ctcagcgttt gagtcagcca ggagtagcaa 2041 taggtttggc ttggactccc ttaggtggag aaatcatgtt cgtggaggcg agtcgaatgg 2101 atggcgaggg ccagttaact ctgaccggcc agctcgggga cgtgatgaag gagtccgccc 2161 acctcgctat cagctggctc cgcagcaacg caaagaagta ccagctgacc aatgcttttg 2221 gaagttttga tcttcttgac aacacagaca tccatctgca cttcccagct ggagctgtca 2281 caaaagatgg accatctgct ggagttacca tagtaacctg tctcgcctca ctttttagtg 2341 ggcggctggt acgttcagat gtagccatga ctggagaaat tacactgaga ggtcttgttc 2401 ttccagtggg tggaattaaa gacaaagtgc tggcggcaca cagagcggga ctgaagcaag 2461 tcattattcc tcggagaaat gaaaaagacc ttgagggaat cccaggcaac gtacgacagg 2521 atttaagttt tgtcacagca agctgcctgg atgaggttct taatgcagct tttgatggtg 2581 gctttactgt caagaccaga cctggtctgt taaattgcaa actgttgcca actttcttgt 2641 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2701 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a