Construct: ORF ccsbBroad304_09680

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002536.1_s304c1, BRDN0000407166
Derived from:
ccsbBroadEn_09680
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
C2CD6 (151254)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09680
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 151254 C2CD6 C2 calcium dependent domain... NM_152525.6 99.8% 99.8% 291G>A;1128T>A
2 human 151254 C2CD6 C2 calcium dependent domain... XM_006712331.3 99.7% 99.6% 291G>A;1128T>A;1161_1163delAGC
3 human 151254 C2CD6 C2 calcium dependent domain... XM_024452728.1 95.8% 95.8% 291G>A;1128T>A;1504_1505ins75
4 human 151254 C2CD6 C2 calcium dependent domain... XM_006712332.3 95.7% 95.6% (many diffs)
5 human 151254 C2CD6 C2 calcium dependent domain... NM_001168217.2 86.2% 81.2% (many diffs)
6 human 151254 C2CD6 C2 calcium dependent domain... XM_006712333.3 75.2% 74% (many diffs)
7 human 151254 C2CD6 C2 calcium dependent domain... XM_006712334.3 75.2% 74% (many diffs)
8 human 151254 C2CD6 C2 calcium dependent domain... XM_006712335.4 74.4% 74% (many diffs)
9 human 151254 C2CD6 C2 calcium dependent domain... NM_001168216.2 62.7% 61.5% (many diffs)
10 human 151254 C2CD6 C2 calcium dependent domain... XM_011510736.2 62.7% 61.5% (many diffs)
11 human 151254 C2CD6 C2 calcium dependent domain... XM_006712336.3 62.4% 61.9% (many diffs)
12 human 151254 C2CD6 C2 calcium dependent domain... NM_001168221.2 32.5% 29.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1935
ORF length:
1869
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gccaccccaa gagacaaata ggcctttcag cacacttgat aaccgcagcg
121ggcaggtcca agtcctgtcc gccaccccgc tattgcagag gaatccctac agcagcccgg
181atatcatgca cattaaaggg tcggaggctt cttcggtccc ttacgccctg aaccagggca
241cgacggccct gcctaagaac aagaaccagg agggcaccgg gcaccggctg ctgaacatgc
301tgagaaaaac tcttaaagag tctgatagtg aagaactgga aataacacag gagacaccaa
361atttggtgcc atttggggat gtggttggct gcctgggcat tcatataaag aactgcagac
421attttatgcc taagatcagt ttacaacatt atgctaattt attcattcgc atctctataa
481acaaagctgt gaaatgtaca aaaatgtgta gcttgctatc caaaaacgat gagaagaaca
541ctgtaattaa gttcgatgaa gtgaagtatt tttctgtaca ggttcccaga cgttatgatg
601ataagcggaa taatatttta ttggaactca tacaatatga caatagagaa aaacgtgctt
661tcttgttagg gagtgttcag atacatcttt atgaggtaat tcagaaaggg tgcttcattg
721aagaggtcca agtgttgcat ggaaacatat ttgtctgcag gctggaggtg gaatttatgt
781tctcctatgg aaactttggt tatggatttt cacatcagtt aaaacctctt cagaaaatta
841ctgagccatc catgtttatg aatcttgcac cacctccaga aagaacagat cccgtgacaa
901aagttattac accacagaca gtagaatatc cagcattctt atccccagac ctgaatgtta
961ctgttgggac tccagctgtg caatcctcca accagccatc tgtagtgcga cttgaaaaac
1021ttcagcaaca accccgggaa aggctggaaa agatgaaaaa agaatataga aatctgaata
1081catggataga taaagctaac tatttagaaa gcattctaat gccaaaattg gaacataagg
1141attctgagga aactaatata gatgaggcat ctgaaaacac gaaaagtaat caacctgaag
1201aggagcttga aaatattgta ggagtagata tccctcttgt aaatgaggaa gctgaaacta
1261ctgcaaatga gttactggat aatgattctg agaaaggcct gactatacca actttgaacc
1321aatcggacca agataattca actgctgatg cttctaaaaa tgatgaatca acaccttcac
1381ccacagaagt gcactcattg tgcactattt ctaaccagga aacaataaaa gcaggcagaa
1441taccaccttt aggtgaacgt caatcagaaa gcatgccaga cagaaaaatg aaaaatgtat
1501tttttccatt agaagtaaaa ttaaaagata actatccaag cattttaaaa gcagactcat
1561ctctatcaga ggtggctttt tcaccaaaag aatataattc accatctttc agaccagaat
1621atatagaatt caaaccaaaa tttcaggatt gttcagataa atttgaggat ttacatgaca
1681tgacctcatt tacacacctt aaaaaagtaa aatcaagatc aaggctttta ggaaaaagct
1741cagatgatat ccataaccat gccagacact ctgcaagacc atatactgct ccggaggtta
1801acaagcaacg agaaagctac agtggaaagt ttacaagtcg tcgaatggtt tcatcaggct
1861tagttcatat aaatgataaa acatcagatt atgaaatgca taaaatgcgg ccaaaaaaaa
1921ttaaaagagg atattgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1981ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2041cgacaatcaa cctctggatt a

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