Construct: ORF ccsbBroad304_10835
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014285.1_s304c1, BRDN0000405160
- Derived from:
- ccsbBroadEn_10835
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABRR2 (2570)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10835
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2570 | GABRR2 | gamma-aminobutyric acid typ... | NM_002043.5 | 100% | 100% | |
| 2 | human | 2570 | GABRR2 | gamma-aminobutyric acid typ... | XM_011535713.2 | 87% | 84.7% | (many diffs) |
| 3 | human | 2570 | GABRR2 | gamma-aminobutyric acid typ... | XM_011535714.3 | 78.7% | 78.7% | 0_1ins297 |
| 4 | human | 2570 | GABRR2 | gamma-aminobutyric acid typ... | XM_011535715.3 | 78.7% | 78.7% | 0_1ins297 |
| 5 | human | 2570 | GABRR2 | gamma-aminobutyric acid typ... | XM_011535716.3 | 78.7% | 78.7% | 0_1ins297 |
| 6 | human | 2570 | GABRR2 | gamma-aminobutyric acid typ... | XM_011535717.3 | 78.7% | 78.7% | 0_1ins297 |
| 7 | mouse | 14409 | Gabrr2 | gamma-aminobutyric acid (GA... | NM_008076.3 | 83.6% | 85.5% | (many diffs) |
| 8 | mouse | 14409 | Gabrr2 | gamma-aminobutyric acid (GA... | XM_006537616.2 | 81% | 84% | (many diffs) |
| 9 | mouse | 14409 | Gabrr2 | gamma-aminobutyric acid (GA... | XM_006537617.3 | 53.8% | 56.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1464
- ORF length:
- 1395
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gccttatttt acaagactca ttttgttctt gttttgcttg atggttctcg 121 tggagagcag aaaacccaag aggaagcgat ggacagggca ggtggaaatg cccaagccaa 181 gtcacttata taagaagaac cttgatgtga ccaagatccg gaagggaaag cctcagcagc 241 ttctcagagt ggacgagcac gacttcagca tgagacccgc cttcggaggc cctgccatcc 301 cggtgggcgt ggacgtacag gtggagagcc tggacagcat ctccgaggtg gacatggact 361 tcactatgac cctgtacctg cggcattact ggaaggatga gaggctagct ttctccagcg 421 ccagcaacaa gagcatgacc ttcgatggcc ggctggtgaa gaagatctgg gtccctgatg 481 tcttctttgt tcactccaaa agatcgttca ctcatgacac caccactgac aacatcatgc 541 tgagggtgtt cccagatgga cacgtgctgt acagcatgag gattacggtc actgccatgt 601 gcaacatgga cttcagccac tttcccctgg actcccagac ctgttctttg gagctggaga 661 gctatgccta tacagatgaa gatctaatgc tgtactggaa gaatggggat gaatccctaa 721 aaacagatga gaagatctcc ttgtctcagt ttctgattca gaaatttcac acaacttcca 781 ggctggcctt ctacagcagc actggctggt acaaccgtct gtacattaac ttcacgttgc 841 gtcgccacat cttcttcttc ttgctccaaa catatttccc tgccactctg atggtcatgc 901 tgtcctgggt gtccttctgg atcgaccgca gagctgtgcc tgccagagtt tcactgggta 961 tcacgacggt gctgaccatg accaccatca tcacgggcgt gaatgcctcc atgccgcgcg 1021 tctcctacgt caaggccgtg gacatctacc tctgggtcag ctttgtgttc gtgttcctct 1081 cggtgctgga gtatgcggct gtcaactacc tgaccaccgt gcaggagcgc aaggaacgga 1141 agctgcggga gaagttcccg tgcatgtgtg gaatgcttca ttcaaaaacc atgatgctgg 1201 atggaagcta cagtgagtct gaggccaaca gcctggctgg gtaccccaga agccatatcc 1261 tgacagaaga agaaaggcaa gacaaaatag tggtccacct gggcctgagt ggtgaagcca 1321 acgctgccag aaagaagggg cttctgaagg gccagacggg ttttcgtatc ttccagaata 1381 cccatgccat tgacaaatac tctaggttga tattccctgc ctcctacata tttttcaact 1441 taatttattg gtcagtgttt tccttgccaa ctttcttgta caaagtggtt ggtaagccta 1501 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1561 gcgttaagtc gacaatcaac ctctggatta