Construct: ORF ccsbBroad304_10863

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013548.1_s304c1, BRDN0000410059
Derived from:
ccsbBroadEn_10863
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRIK2 (2898)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10863
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2898 GRIK2 glutamate ionotropic recept... XM_017010782.2 56.4% 52.3% (many diffs)
2 human 2898 GRIK2 glutamate ionotropic recept... XM_017010781.2 47.9% 44.6% (many diffs)
3 human 2898 GRIK2 glutamate ionotropic recept... NM_175768.3 39% 36.4% (many diffs)
4 human 2898 GRIK2 glutamate ionotropic recept... XM_024446411.1 39% 36.4% (many diffs)
5 human 2898 GRIK2 glutamate ionotropic recept... NM_001166247.1 38% 35.5% (many diffs)
6 human 2898 GRIK2 glutamate ionotropic recept... XM_011535777.3 38% 35.5% (many diffs)
7 human 2898 GRIK2 glutamate ionotropic recept... XM_024446410.1 38% 35.5% (many diffs)
8 human 2898 GRIK2 glutamate ionotropic recept... NM_021956.4 37.3% 34.9% (many diffs)
9 human 2898 GRIK2 glutamate ionotropic recept... XM_005266945.2 37.3% 34.9% (many diffs)
10 human 2898 GRIK2 glutamate ionotropic recept... XM_005266946.4 32% 29.6% (many diffs)
11 human 2898 GRIK2 glutamate ionotropic recept... XR_002956278.1 20.6% (many diffs)
12 human 7576 ZNF28 zinc finger protein 28 NM_001369767.1 7.6% 1.9% (many diffs)
13 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243127.2 48.1% 48.9% (many diffs)
14 mouse 14806 Grik2 glutamate receptor, ionotro... NM_010349.2 34.9% 35.6% (many diffs)
15 mouse 14806 Grik2 glutamate receptor, ionotro... XM_006512545.3 34% 34.7% (many diffs)
16 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243124.2 33.6% 34.3% (many diffs)
17 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243125.2 33.6% 34.3% (many diffs)
18 mouse 14806 Grik2 glutamate receptor, ionotro... NM_001111268.1 33.4% 34.1% (many diffs)
19 mouse 14806 Grik2 glutamate receptor, ionotro... XM_017313804.1 33.4% 34.1% (many diffs)
20 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243123.2 32.7% 33.4% (many diffs)
21 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243120.2 32.2% 32.9% (many diffs)
22 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243121.2 32.2% 32.9% (many diffs)
23 mouse 14806 Grik2 glutamate receptor, ionotro... XM_011243122.2 32.2% 32.9% (many diffs)
24 mouse 14806 Grik2 glutamate receptor, ionotro... XR_001779465.1 30.4% (many diffs)
25 mouse 14806 Grik2 glutamate receptor, ionotro... XR_001779464.1 27.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1125
ORF length:
1059
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gattattttc ccgattctaa gtaatccagt cttcaggcgc accgttaaac
121tcctgctctg tttactgtgg attggatatt ctcaaggaac cacacatgta ttaagatttg
181gtggtatttt tgaatatgtg gaatctggcc caatgggagc tgaggaactt gcattcagat
241ttgctgtgaa cacaattaac agaaacagaa cattgctacc caatactacc cttacctatg
301atacccagaa gataaacctt tatgatagtt ttgaagcatc caagaaagcc tgtgatcagc
361tgtctcttgg ggtggctgcc atcttcgggc cttcacacag ctcatcagca aacgcagtgc
421agtccatctg caatgctctg ggagttcccc acatacagac ccgctggaag caccaggtgt
481cagacaacaa agattccttc tatgtcagtc tctacccaga cttctcttca ctcagccgtg
541ccattttaga cctggtgcag tttttcaagt ggaaaaccgt cacggttgtg tatgatgaca
601gcactggtct cattcgtttg caagagctca tcaaagctcc atcaaggtat aatcttcgac
661tcaaaattcg tcagttacct gctgatacaa aggatgcaaa acccttacta aaagaaatga
721aaagaggcaa ggagtttcat gtaatctttg attgtagcca tgaaatggca gcaggcattt
781taaaacaggc attagctatg ggaatgatga cagaatacta tcattatatc tttaccactc
841tggacctctt tgctcttgat gttgagccct accgatacag tggtgttaac atgacagggt
901tcagaatatt aaatacagaa aatacccaag tctcctccat cattgaaaag tggtcgatgg
961aacgattgca ggcacctccg aaacccgatt caggtttgct ggatggattt atgacgatgg
1021agtttcactc ttgttgccca ggctggagtg caatggcgcg atctcacctc actgcaacct
1081ctgcctcctg ggttcaagcg attcttctgc ctcagcctcc cgagtaccca actttcttgt
1141acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1201gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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