Transcript: Mouse XM_011243123.2

PREDICTED: Mus musculus glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Grik2 (14806)
Length:
10035
CDS:
799..3585

Additional Resources:

NCBI RefSeq record:
XM_011243123.2
NBCI Gene record:
Grik2 (14806)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011243123.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000100274 CGTTTATGACTCTTGGAATAA pLKO.1 2393 CDS 100% 13.200 10.560 N Grik2 n/a
2 TRCN0000100271 GCCGTTTATGACTCTTGGAAT pLKO.1 2391 CDS 100% 4.950 3.960 N Grik2 n/a
3 TRCN0000100272 CCTCCGATTATGCTTTCTTAA pLKO.1 3092 CDS 100% 13.200 9.240 N Grik2 n/a
4 TRCN0000100273 GCATTCAGATTTGCTGTGAAT pLKO.1 964 CDS 100% 0.495 0.347 N Grik2 n/a
5 TRCN0000100270 GTTATCAACATGCACACATTT pLKO.1 3710 3UTR 100% 13.200 7.920 N Grik2 n/a
6 TRCN0000166364 CACACACACACACACACACAA pLKO.1 5582 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011243123.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13864 pDONR223 100% 57.3% 61.5% None (many diffs) n/a
2 ccsbBroad304_13864 pLX_304 0% 57.3% 61.5% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000471328 TCAAACTAGATTACAATTCGATTC pLX_317 18.9% 57.3% 61.5% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_10863 pDONR223 100% 32.7% 33.4% None (many diffs) n/a
5 ccsbBroad304_10863 pLX_304 0% 32.7% 33.4% V5 (many diffs) n/a
6 TRCN0000471009 TTGCTGATAGATCAATTCTGAAGC pLX_317 31.6% 32.7% 33.4% V5 (many diffs) n/a
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