Construct: ORF ccsbBroad304_11018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001189.1_s304c1, BRDN0000462030
- Derived from:
- ccsbBroadEn_11018
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCCA (5095)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11018
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020609.1 | 98.6% | 98.5% | (many diffs) |
| 2 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_000282.4 | 96.5% | 96.5% | 1_75del;627A>G |
| 3 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001352605.2 | 94% | 94% | 1_75del;627A>G;1844_1845ins54 |
| 4 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001127692.2 | 92.9% | 92.9% | 1_75del;105_106ins78;549A>G |
| 5 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001178004.1 | 90% | 90.1% | 1_75del;627A>G;1898_1899ins141 |
| 6 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001352606.2 | 89.9% | 89.9% | 1_75del;627A>G;1063_1064ins144 |
| 7 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_011521093.1 | 86.7% | 83.2% | (many diffs) |
| 8 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001352607.2 | 86.4% | 86.5% | (many diffs) |
| 9 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001352608.2 | 86.3% | 86.4% | (many diffs) |
| 10 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020607.1 | 85.4% | 80.6% | (many diffs) |
| 11 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XR_001749574.1 | 84.8% | (many diffs) | |
| 12 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001352609.2 | 84.3% | 83.6% | (many diffs) |
| 13 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020605.1 | 83.9% | 79.3% | (many diffs) |
| 14 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020612.1 | 83.4% | 79.3% | (many diffs) |
| 15 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NR_148030.2 | 82% | (many diffs) | |
| 16 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020611.1 | 82% | 82.3% | (many diffs) |
| 17 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020613.1 | 81.8% | 78.5% | (many diffs) |
| 18 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NR_148031.2 | 80.7% | (many diffs) | |
| 19 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020606.1 | 80.5% | 75.9% | (many diffs) |
| 20 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XR_001749567.1 | 79.9% | (many diffs) | |
| 21 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020615.1 | 78.9% | 77.1% | (many diffs) |
| 22 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NR_148028.2 | 78% | (many diffs) | |
| 23 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XR_001749568.1 | 77.4% | (many diffs) | |
| 24 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NR_148027.2 | 76.5% | (many diffs) | |
| 25 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NR_148029.2 | 75% | (many diffs) | |
| 26 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XR_931615.1 | 72.8% | (many diffs) | |
| 27 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XR_001749569.1 | 72.2% | (many diffs) | |
| 28 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XM_017020616.1 | 71.8% | 71.8% | (many diffs) |
| 29 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XR_001749576.1 | 68.2% | (many diffs) | |
| 30 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | XR_001749577.1 | 64.1% | (many diffs) | |
| 31 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001352610.2 | 58.7% | 58.7% | 0_1ins870 |
| 32 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001352611.2 | 56.1% | 56.1% | 0_1ins870;899_900ins54 |
| 33 | human | 5095 | PCCA | propionyl-CoA carboxylase s... | NM_001352612.2 | 51.9% | 51.9% | 0_1ins870;118_119ins144 |
| 34 | mouse | 110821 | Pcca | propionyl-Coenzyme A carbox... | NM_144844.2 | 84.7% | 87.8% | (many diffs) |
| 35 | mouse | 110821 | Pcca | propionyl-Coenzyme A carbox... | XM_006518430.3 | 82.4% | 85.3% | (many diffs) |
| 36 | mouse | 110821 | Pcca | propionyl-Coenzyme A carbox... | XM_006518432.3 | 80.3% | 84.6% | (many diffs) |
| 37 | mouse | 110821 | Pcca | propionyl-Coenzyme A carbox... | XR_001781088.1 | 73.3% | (many diffs) | |
| 38 | mouse | 110821 | Pcca | propionyl-Coenzyme A carbox... | XM_011244922.2 | 47.5% | 48.5% | (many diffs) |
| 39 | mouse | 110821 | Pcca | propionyl-Coenzyme A carbox... | XR_001781087.1 | 17.7% | (many diffs) | |
| 40 | mouse | 110821 | Pcca | propionyl-Coenzyme A carbox... | XR_383133.3 | 16.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2175
- ORF length:
- 2109
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gagcgcggcg ctgcggaccc tgaagcatgt tctgtactat tcaagacagt 121 gcttaatggt gtcccgtaat cttggttcag tgggatatga tcctaatgaa aaaacttttg 181 ataaaattct tgttgctaat agaggagaaa ttgcatgtcg ggttattaga acttgcaaga 241 agatgggcat taagacagtt gccatccaca gtgatgttga tgctagttct gttcatgtga 301 aaatggcgga tgaggctgtc tgtgttggcc cagctcccac cagtaaaagc tacctcaaca 361 tggatgccat catggaagcc attaagaaaa ccagggccca agctgtacat ccaggttatg 421 gattcctttc agaaaacaaa gaatttgcca gatgtttggc agcagaagat gtcgttttca 481 ttggacctga cacacatgct attcaagcca tgggcgacaa gattgaaagc aaattattag 541 ctaagaaagc agaggttaat acaatccctg gctttgatgg agtagtcaag gatgcagaag 601 aagctgtcag aattgcgagg gaaattggct accctgtcat gatcaaggcc tcagcaggtg 661 gtggtgggaa aggcatgcgc attgcttggg atgatgaaga gaccagggat ggttttagat 721 tgtcatctca agaagctgct tctagttttg gcgatgatag actactaata gaaaaattta 781 ttgataatcc tcgtcatata gaaatccagg ttctaggtga taaacatggg aatgctttat 841 ggcttaatga aagagagtgc tcaattcaga gaagaaatca gaaggtggtg gaggaagcac 901 caagcatttt tttggatgcg gagactcgaa gagcgatggg agaacaagct gtagctcttg 961 ccagagcagt aaaatattcc tctgctggga ccgtggagtt ccttgtggac tctaagaaga 1021 atttttattt cttggaaatg aatacaagac tccaggttga gcatcctgtc acagaatgca 1081 ttactggcct ggacctagtc caggaaatga tccgtgttgc taagggctac cctctcaggc 1141 acaaacaagc tgatattcgc atcaacggct gggcagttga atgtcgggtt tatgctgagg 1201 acccctacaa gtcttttggt ttaccatcta ttgggagatt gtctcagtac caagaaccgt 1261 tacatctacc tggtgtccga gtggacagtg gcatccaacc aggaagtgat attagcattt 1321 attatgatcc tatgatttca aaactaatca catatggctc tgatagaact gaggcactga 1381 agagaatggc agatgcactg gataactatg ttattcgagg tgttacacat aatattgcat 1441 tacttcgaga ggtgataatc aactcacgct ttgtaaaagg agacatcagc actaaatttc 1501 tctccgatgt gtatcctgat ggcttcaaag gacacatgct aaccaagagt gagaagaacc 1561 agttattggc aatagcatca tcattgtttg tggcattcca gttaagagca caacattttc 1621 aagaaaattc aagaatgcct gttattaaac cagacatagc caactgggag ctctcagtaa 1681 aattgcatga taaagttcat accgtagtag catcaaacaa tgggtcagtg ttctcggtgg 1741 aagttgatgg gtcgaaacta aatgtgacca gcacgtggaa cctggcttcg cccttattgt 1801 ctgtcagcgt tgatggcact cagaggactg tccagtgtct ttctcgagaa gcaggtggaa 1861 acatgagcat tcagtttctt ggtacagtgt acaaggtgaa tatcttaacc agacttgccg 1921 cagaattgaa caaatttatg ctggaaaaag tgactgagga cacaagcagt gttctgcgtt 1981 ccccgatgcc cggagtggtg gtggccgtct ctgtcaagcc tggagacgcg gtagcagaag 2041 gtcaagaaat ttgtgtgatt gaagccatga aaatgcagaa tagtatgaca gctgggaaaa 2101 ctggcacggt gaaatctgtg cactgtcaag ctggagacac agttggagaa ggggatctgc 2161 tcgtggagct ggaatgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 2221 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2281 cgacaatcaa cctctggatt a