Construct: ORF ccsbBroad304_11706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017350.1_s304c1, BRDN0000407242
- Derived from:
- ccsbBroadEn_11706
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND5A (23258)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11706
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23258 | DENND5A | DENN domain containing 5A | NM_001348750.2 | 73.2% | 73.2% | 1_957del |
| 2 | human | 23258 | DENND5A | DENN domain containing 5A | NM_001348749.2 | 69% | 69% | 1_1173del |
| 3 | human | 23258 | DENND5A | DENN domain containing 5A | NM_015213.4 | 67.7% | 67.7% | 1_1245del |
| 4 | human | 23258 | DENND5A | DENN domain containing 5A | NM_001243254.2 | 64.1% | 62.2% | 1_1245del;3645_3646ins34;3723_3724ins104 |
| 5 | human | 23258 | DENND5A | DENN domain containing 5A | NR_145966.2 | 50.9% | 1_1502del;3561_3562ins83;4036_4884del | |
| 6 | mouse | 19347 | Dennd5a | DENN/MADD domain containing 5A | NM_001311139.1 | 62% | 67.5% | (many diffs) |
| 7 | mouse | 19347 | Dennd5a | DENN/MADD domain containing 5A | NM_021494.1 | 60.9% | 66.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2682
- ORF length:
- 2616
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc atttggaatt ccccctgaag ggaatcttca ttgcagtgag agtgcctcca 121 agctgaagag gctgcgggcc tctgagcttg tctcggacaa gaggaatggg aacattgctg 181 gctccccttt gcattcctac gagcttctta aggagaatga aactattgcc cggctgcaag 241 ccttggtcaa gagaactggg gtgagcctgg aaaagttgga agtgcgtgaa gaccccagca 301 gcaataagga tctcaaagtt cagtgtgatg aagaagaact caggatttac cagctaaaca 361 ttcagatccg ggaagttttt gcaaatcgtt tcactcagat gtttgcagat tatgaggtgt 421 ttgtcatcca acccagccag gataaggaat cctggtttac caacagggag caaatgcaaa 481 actttgataa agcatctttt ctgtcagatc agcctgagcc ctacctgccc ttcctctcaa 541 gattcctgga gacccagatg tttgcatctt tcattgacaa caaaataatg tgtcatgatg 601 atgatgataa agaccctgta ctccgggtat ttgattcccg agttgacaag atcaggctgt 661 tgaatgttcg gacacctact ctccgtacat ccatgtacca gaagtgtacc actgtggatg 721 aagcagagaa agcaattgag ctgcgtctgg caaaaattga ccatactgca attcacccac 781 atttacttga catgaagatt ggacaaggga aatatgagcc gggcttcttc cctaagctgc 841 agtctgatgt actttccact gggccagcca gcaacaagtg gacgaaaagg aatgcccctg 901 cccagtggag gcggaaagat cggcagaagc agcacacaga acacctgcgt ttagataatg 961 accagaggga gaagtacatc caggaagcca ggactatggg cagcactatc cgccagccca 1021 aactgtccaa cctctctcca tcagtgattg cccagaccaa ttggaagttt gtagagggcc 1081 tgctgaagga atgccgcaat aagaccaaga ggatgctggt ggaaaagatg ggccgagaag 1141 ctgtggagct agggcatggg gaggtgaaca tcacaggggt ggaagagaac accctgattg 1201 ccagcctttg tgatctcctg gaaaggatct ggagtcatgg actacaagtg aaacagggga 1261 aatcagcctt atggtcccac ctgttacatt atcaggacaa ccggcagaga aaactcacat 1321 caggaagcct cagtacctca ggaatacttc ttgattcaga acgtaggaag tctgatgcca 1381 gctcactcat gcctcccctg aggatctccc tgattcagga tatgaggcac atccagaaca 1441 tcggggaaat caagactgat gtgggaaagg ccagagcatg ggtgcgactg tccatggaaa 1501 aaaagttact ttccagacac ctgaagcagc tcctctcaga ccatgagctc accaaaaagt 1561 tatataagcg ctatgccttc ctgcgctgtg atgacgagaa ggagcagttc ctctatcacc 1621 tcctgtcttt caatgccgtc gattactttt gcttcaccaa tgtcttcaca actatcctga 1681 tcccgtacca cattctgatc gtaccaagca agaagctggg gggctccatg ttcactgcca 1741 acccatggat ctgtatatca ggagaattgg gtgagacaca gatcatgcag attcccagga 1801 atgtgctaga gatgaccttc gagtgccaga acttggggaa gcttactact gtccagattg 1861 gccatgataa ctctgggctg tatgccaaat ggctggtgga gtatgtgatg gtcaggaatg 1921 agatcacagg acatacctac aagttcccgt gtggccggtg gttagggaag ggcatggatg 1981 atggaagcct ggagcggatc ctagttgggg agctgctcac atcccagcct gaggtggatg 2041 agaggccatg ccggaccccg ccgctgcagc agtcccccag tgtcatccgg aggcttgtta 2101 ccatctcacc caacaacaag cccaagctga acactgggca gatccaggag tccatcgggg 2161 aggcagtcaa tggcattgtg aagcacttcc ataagcctga gaaagagcga ggcagtctga 2221 cgctgttgct ctgtggagag tgtggccttg tctcggcctt ggaacaggct ttccagcatg 2281 gatttaaatc gccccggctc ttcaaaaatg tcttcatttg ggatttcctg gaaaaagcac 2341 aaacctatta tgagacatta gagaagaatg aagtagtccc tgaggaaaac tggcatacaa 2401 gagcccggaa cttctgccga tttgtcactg caatcaacaa tactccccgg aacatcggca 2461 aggatggcaa gtttcagatg ctggtgtgct tgggagccag agatcacctc ctacaccact 2521 ggattgccct gctggctgac tgccccatca ctgcacacat gtatgaggat gtggcactga 2581 tcaaagacca tacacttgtc aattccttga ttcgtgtgct gcagacattg caggagttca 2641 acatcacgct ggagacgtcc cttgtcaagg gcatcgacat cagcccaact ttcttgtaca 2701 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2761 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta