Construct: ORF ccsbBroad304_12193
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010866.1_s304c1, BRDN0000410638
- Derived from:
- ccsbBroadEn_12193
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASXL2 (55252)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12193
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55252 | ASXL2 | ASXL transcriptional regula... | NM_001369347.1 | 78% | 78% | 1607C>T;1727_2497del |
| 2 | human | 55252 | ASXL2 | ASXL transcriptional regula... | NM_001369346.1 | 66.6% | 66.5% | 1_606del;2213C>T;2333_3103del |
| 3 | human | 55252 | ASXL2 | ASXL transcriptional regula... | NM_018263.6 | 63.9% | 63.9% | 1_780del;2387C>T;2507_3277del |
| 4 | mouse | 75302 | Asxl2 | additional sex combs like 2... | XM_006515242.2 | 65.8% | 61.9% | (many diffs) |
| 5 | mouse | 75302 | Asxl2 | additional sex combs like 2... | XM_006515241.2 | 64.8% | 61% | (many diffs) |
| 6 | mouse | 75302 | Asxl2 | additional sex combs like 2... | XM_017315231.1 | 61.6% | 57.9% | (many diffs) |
| 7 | mouse | 75302 | Asxl2 | additional sex combs like 2... | XM_017315232.1 | 61.6% | 57.9% | (many diffs) |
| 8 | mouse | 75302 | Asxl2 | additional sex combs like 2... | NM_172421.5 | 56% | 52.6% | (many diffs) |
| 9 | mouse | 75302 | Asxl2 | additional sex combs like 2... | XM_017315230.1 | 56% | 52.6% | (many diffs) |
| 10 | mouse | 75302 | Asxl2 | additional sex combs like 2... | XM_006515239.3 | 54.1% | 50.9% | (many diffs) |
| 11 | mouse | 75302 | Asxl2 | additional sex combs like 2... | NM_001270988.1 | 54.1% | 50.9% | (many diffs) |
| 12 | mouse | 75302 | Asxl2 | additional sex combs like 2... | XM_017315229.1 | 54.1% | 50.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2820
- ORF length:
- 2754
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa aagaactaaa tgtgctgaca ttgacgttga gacaccggac tccattctgg 121 ttaatacaaa tctgcgagca ctgatcaaca agcacacatt ttcagtcctt cctggagatt 181 gccagcaacg actgctttta ctactcccag aggtagatcg acaggttggt ccagatggtt 241 taatgaagtt aaatggctca gcccttaaca atgaattctt cacttcagca gcccaaggct 301 ggaaggaaag actctcagaa ggtgagttta cacctgagat gcaggtgaga attcgacaag 361 agattgagaa ggagaaaaaa gtggagccat ggaaagaaca attctttgaa agctactatg 421 ggcagagttc tggcctgagc cttgaagatt ctaagaaatt gacagcttct cccagtgatc 481 ccaaagtaaa gaaaacccca gctgaacaac caaaatccat gcctgtgtca gaggcctctc 541 ttatcagaat agttccagta gtctcccagt cagagtgtaa agaagaagca ttgcaaatgt 601 catcaccagg cagaaaagaa gagtgtgaaa gccaaggtga agtgcagccg aacttctcca 661 catcttcaga gcccctgctt tcctcagctc tcaatacaca tgagcttagc agcattcttc 721 ccatcaagtg cccaaaggat gaggatctct tggagcagaa gccagtcacc tctgctgaac 781 aggaatctga gaagaaccat ctcaccacag cttctaatta taacaaaagt gaaagccaag 841 aatctttagt tacatcgcca agcaaaccca agagtcctgg ggttgaaaaa ccaatagtga 901 agcccacagc aggagcgggt ccacaggaga ctaatatgaa agaacctcta gcaactcttg 961 ttgatcagag cccagaaagc ctcaagagga agtcttccct cacccaagaa gaggcccctg 1021 tgagctggga gaagaggcca cgtgtcactg agaatcgcca gcaccagcag ccatttcagg 1081 tctcaccaca gccctttctc aatagagggg acagaatcca ggtgcgaaaa gtaccacctc 1141 tcaagatccc ggtctccaga atctccccca tgccgtttca tccatcgcag gtctctccca 1201 gggctcgttt tccagtctcc atcactagtc ctaacagaac aggagccaga actcttgcag 1261 acatcaaagc aaaagcccaa ctggtcaaag cacagagggc agcagctgcc gctgccgccg 1321 cagctgctgc agccgcctca gttggaggga ccattccagg acctggccca gggggtggac 1381 aaggtccagg agagggtggt gaagggcaga ctgctagagg aggcagtcca ggctcagaca 1441 gagtcagtga aactggaaag ggccccacac tggaactggc aggaactgga agcaggggag 1501 gtacgagaga gcttttaccc tgtggtccag agactcagcc ccagtctgag accaagacca 1561 ccccaagcca ggcacagcct catagtgtct ctggagcaca actacagcaa acccccccag 1621 tgcctccaac acctgccgtc agtggagcat gcacaagtgt cccatcacca gtccacatag 1681 agaaattgga taatgaaaaa ctgaacccca ccagagcaac agccacagtg gcctctgtca 1741 gccatccaca agggcccagt agttgcagac aggagaaagc accttctcca acaggtttcc 1801 agctggaaga catctccaca agccagaggt tcatgctggg ttttgctggc agaaggacat 1861 ccaaacctgc aatggcaggg cactacttac tgaatatttc tacctacggc cggggctcag 1921 agagctttag gaggacccat tctgtaaacc ctgaagatcg tttttgtcta agcagcccca 1981 ctgaagcctt gaaaatggga tatacagact gtaaaaatgc aacaggagag agtagcagca 2041 gcaaagaaga tgacactgat gaggaaagta ctggtgatga gcaggaatct gtcacagtga 2101 aagaggagcc ccaggtttcc cagagtgctg gcaagggtga cacaagttca ggacctcaca 2161 gcagggaaac tctatctacc agtgattgct tagctagcaa gaatgtgaag gctgagatac 2221 cattgaatga gcaaaccact ttaagtaagg agaattacct gttcactaga ggccaaacat 2281 ttgatgaaaa gaccctagcc agagatttaa ttcaggcagc acagaagcag atggctcatg 2341 cagtgagagg taaggcaatc cgtagcagcc ccgagctttt cagttctact gttcttcctc 2401 tgcctgcaga cagccccacc caccagcccc tactccttcc acccctgcaa accccgaagt 2461 tgtatggaag ccccacccag atagggccaa gctatagagg catgatcaat gtctccacct 2521 catctgacat ggaccataac tctgctgtac caggtagcca ggtatctagc aatgtaggtg 2581 atgtcatgtc attttcagtg actgtcacta ccatccctgc tagccaagct atgaatccca 2641 gcagccatgg ccagaccatt cctgttcagg cgttctccga agagaacagc atagagggca 2701 cgccttcgaa atgttactgc cgcttgaaag ccatgatcat gtgcaaaggc tgtggcgctt 2761 tctgccatga tgattgcatc ggcccctcca aactgtgcgt ctcctgcctt gtcgttcggt 2821 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 2881 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 2941 ggatta