Construct: ORF ccsbBroad304_12277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011538.1_s304c1, BRDN0000406705
- Derived from:
- ccsbBroadEn_12277
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST6GALNAC1 (55808)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12277
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | XM_017024843.1 | 93.4% | 92.3% | 493C>A;1137G>A;1309_1310ins90 |
| 2 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | XM_011524996.2 | 85.8% | 75.1% | (many diffs) |
| 3 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | XM_011524995.3 | 83.2% | 73% | (many diffs) |
| 4 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | XM_017024842.1 | 83.2% | 73% | (many diffs) |
| 5 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | NM_018414.5 | 78.3% | 68.9% | (many diffs) |
| 6 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | XM_017024844.2 | 65.4% | 64.3% | (many diffs) |
| 7 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | NR_110309.2 | 59.1% | (many diffs) | |
| 8 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | XR_002958047.1 | 57.7% | (many diffs) | |
| 9 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | NM_001289107.2 | 56.3% | 48.1% | (many diffs) |
| 10 | human | 55808 | ST6GALNAC1 | ST6 N-acetylgalactosaminide... | XR_001752559.2 | 55.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1479
- ORF length:
- 1413
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gtcctgcctg tggagatgca ggcacctgag ccaaggcgtc cagtggtcct 121 tgcttctggc tgtcctggtc ttctttctct tcgccttgcc ctcttttatt aaggagcctc 181 aaacaaagcc ttccaggcat caacgcacag agaacattaa agaaaggtct ctacagtccc 241 tggcaaagcc taagtcccag gcacccacaa gggcaaggag gacaaccatc tatgcagagc 301 cagtgccaga gaacaatgcc ctcaacacac aaacccagcc caaggcccac accaccggag 361 acagaggaaa ggaggccaac caggcaccgc cggaggagca ggacaaggtg ccccacacag 421 cacagagggc agcatggaag agcccagaaa aagagaaaac catggtgaac acactgtcac 481 ccagagggca agatgcaggg atggcctctg gcaggacaga ggcacaatca tggaagagcc 541 aggacacaaa gacgaccaaa ggaaatgggg gccagaccag gaagctgacg gcctccagga 601 cggtgtcaga gaagcaccag ggcaaagcgg caaccacagc caagacgctc attcccaaaa 661 gtcagcacag aatgctggct cccacaggag cagtgtcaac aaggacgaga cagaaaggag 721 tgaccacagc agtcatccca cctaaggaga agaaacctca ggccacccca ccccctgccc 781 ctttccagag ccccacgacg cagagaaacc aaagactgaa ggccgccaac ttcaaatctg 841 agcctcggtg ggattttgag gaaaaataca gcttcgaaat aggaggcctt cagacgactt 901 gccctgactc tgtgaagatc aaagcctcca agtcgctgtg gctccagaaa ctctttctgc 961 ccaacctcac tctcttcctg gactccagac acttcaacca gagtgagtgg gaccgcctgg 1021 aacactttgc accacccttt ggcttcatgg agctcaacta ctccttggtg cagaaggtcg 1081 tgacacgctt ccctccagtg ccccagcagc agctgctcct ggccagcctc cccgctggga 1141 gcctccggtg catcacctgt gccgtggtgg gcaacggggg catcctgaac aactcccaca 1201 taggccagga gatagacagt cacgactacg tgttccgatt gagcggagct ctcattaaag 1261 gctacgaaca ggatgtgggg actcggacat ccttctacgg ctttaccgcc ttctccctga 1321 cccagtcact ccttatattg ggcaatcggg gtttcaagaa cgtgcctctt gggaaggcac 1381 agaccccagg aagcttttcg ggaagccctg cacatggaca ggtacctgtt gctgcaccca 1441 gactttctcc gatacatgaa gaacaggttt ctgaggtcta cccaactttc ttgtacaaag 1501 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1561 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta