Construct: ORF ccsbBroad304_13006
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010277.1_s304c1, BRDN0000462113
- Derived from:
- ccsbBroadEn_13006
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP12 (94134)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13006
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270695.1 | 100% | 100% | |
| 2 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_018287.7 | 99.4% | 99.4% | 1372_1386del |
| 3 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270696.2 | 97% | 97% | 1296_1297ins75 |
| 4 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270699.1 | 94.4% | 94.4% | 946_947ins141 |
| 5 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | XM_005252644.2 | 94.4% | 94.4% | 946_947ins141 |
| 6 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270697.1 | 93.8% | 93.8% | 946_947ins141;1231_1245del |
| 7 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270698.2 | 91.4% | 91.4% | 946_947ins141;1155_1156ins75 |
| 8 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | XM_011519761.2 | 90.6% | 90.6% | 946_947ins222;1150_1164del |
| 9 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | XM_017016954.2 | 88.2% | 88.2% | 946_947ins222;1074_1075ins75 |
| 10 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | NM_001039692.1 | 88.8% | 91.2% | (many diffs) |
| 11 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_006526314.1 | 88.2% | 90.6% | (many diffs) |
| 12 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_011247018.1 | 86.8% | 89.2% | (many diffs) |
| 13 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | NM_029277.2 | 86.5% | 89.2% | (many diffs) |
| 14 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_017318002.1 | 83.6% | 86.3% | (many diffs) |
| 15 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_006526315.1 | 83.1% | 85.8% | (many diffs) |
| 16 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_006526316.1 | 81.3% | 84.4% | (many diffs) |
| 17 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_017318001.1 | 74.5% | 76.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2592
- ORF length:
- 2523
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaatggct gacagaagtg ggaagattat tccaggacaa gtgtatattg 121 aggtggaata tgattatgaa tatgaagcaa aggacagaaa gattgtgata aaacaagggg 181 agaggtacat cttggtgaaa aagaccaatg atgactggtg gcaagtcaag ccagatgaaa 241 actccaaagc gttttatgtg ccagcccagt atgtgaagga ggtcacgcgc aaagctctca 301 tgccacctgt taagcaggta gctggtctgc caaataactc cacgaaaata atgcagagtt 361 tgcatcttca gagatcaaca gaaaatgtga acaaattgcc tgagctttca agtttcggaa 421 agccatcgtc atctgttcaa ggaacaggtc ttattcgtga tgccaatcag aattttggac 481 ccagttataa tcaaggtcag actgtcaacc taagcctgga cctgacccat aataacggaa 541 agtttaacaa tgactcacat tctcctaaag tttccagcca gaataggaca cgctcatttg 601 gtcattttcc cggtccagag ttcttggatg tagagaaaac tagcttctcc caggaacaat 661 cttgtgattc cgcaggagaa ggctctgaaa gaatacatca agattctgaa tctggtgatg 721 aacttagcag cagctccact gaacagataa gggcaaccac acctccaaat caaggaaggc 781 cagattctcc tgtctatgct aaccttcaag aactgaaaat atcccagtct gctcttcccc 841 cacttcctgg gagcccggca attcagatta atggagaatg ggaaactcat aaagacagct 901 cagggcgttg ctattactat aacagaggga cacaggaaag aacttggaaa cctcctcgtt 961 ggactcggga tgcaagcatc agcaaaggag atttccaaaa tccaggggat caagagcttc 1021 tttcatcgga agaaaactac tacagcactt cttacagcca gtcagatagt cagtgtggtt 1081 ctcctccaag gggttggtca gaagagttgg atgaacgtgg gcatacctta tataccagtg 1141 actatactaa tgaaaagtgg ctcaagcatg ttgatgatca aggtagacaa tattactaca 1201 gtgcagacgg atctcggtca gaatgggaat tgccaaagta taatgcttca tcccagcagc 1261 aaagagaaat aattaaaagt aggagcctgg acaggcggct gcaagaacca atagtattaa 1321 caaagtggag acatagcacc attgtattgg acactaatga taaggaatct ccaactgcct 1381 caaaaccctg ctttcctgaa aatgagtctt ctccctcctc accaaagcac caagatacag 1441 atcaagagaa atatggatta ttaaatgtaa caaaaattgc tgaaaatggg aaaaaggttc 1501 gaaagaactg gttgtcttct tgggcggtgt tgcagggttc atctttactt tttaccaaaa 1561 ctcaaggaag tagcacaagt tggtttggca gtaatcagtc caaaccagag ttcacagtgg 1621 acctcaaggg ggcaacaatt gagatggctt caaaggataa atccagcaaa aagaatgtat 1681 ttgagctgaa aactcgtcaa ggaacagaac tgctaattca gtctgacaat gacactgtta 1741 ttaatgattg gtttaaagtt cttagtagta caatcaataa tcaggcagta gaaactgatg 1801 aaggaattga agaggagata ccggattcac caggaataga aaagcatgat aaagaaaagg 1861 aacaaaagga tcccaaaaag cttcgttcct ttaaagtatc tagcatagat tcttcagaac 1921 agaaaaaaac caagaaaaac ttaaagaagt ttcttacacg acgccccact ttgcaagctg 1981 ttcgtgaaaa aggttatatt aaagatcagg tatttggatc caatctcgct aatctgtgtc 2041 agagagagaa tggcacagta ccaaagtttg tgaagttatg tattgaacat gttgaagaac 2101 atggtttgga tattgatggg atatacagag taagtggcaa cctcgcagtg atccagaaac 2161 taaggtttgc agtcaatcat gatgagaaat tggacttgaa tgacagtaaa tgggaagata 2221 ttcatgtcat tactggagcc ctcaaaatgt tttttcgaga attaccagaa cctcttttta 2281 catttaatca ttttaatgat tttgttaatg caattaagca agaaccaaga cagcgagtcg 2341 ctgctgttaa ggacctaatc agacagttgc caaagccaaa ccaagacaca atgcagattc 2401 ttttccgaca tctcagaaga gttatagaaa atggagagaa aaatcgaatg acctatcaga 2461 gtatagcaat tgtttttggt cccactctat taaaaccaga aaaagagact ggtaatatag 2521 cagttcatac tgtgtaccag aatcagattg tagaattaat tcttctggaa ctgagttcca 2581 tcttcggacg tttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2641 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2701 caatcaacct ctggatta