Construct: ORF ccsbBroad304_13018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014070.1_s304c1, BRDN0000409124
- Derived from:
- ccsbBroadEn_13018
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASC4 (113201)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13018
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113201 | CASC4 | cancer susceptibility 4 | XM_017021880.2 | 64.8% | 60.5% | 482_572del;623_819del |
| 2 | human | 113201 | CASC4 | cancer susceptibility 4 | NM_177974.3 | 46.5% | 43.5% | 482_572del;623_1140del |
| 3 | human | 113201 | CASC4 | cancer susceptibility 4 | NM_138423.4 | 40.5% | 37.9% | 482_572del;623_1308del |
| 4 | human | 113201 | CASC4 | cancer susceptibility 4 | NR_157849.2 | 10.2% | 1_311del;793_883del;934_5198del | |
| 5 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_199038.3 | 47% | 47.2% | (many diffs) |
| 6 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_001205370.1 | 42.9% | 43.1% | (many diffs) |
| 7 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_177054.5 | 40.4% | 40.6% | (many diffs) |
| 8 | mouse | 319996 | Casc4 | cancer susceptibility candi... | NM_001205369.1 | 37.3% | 37.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gggtttcggg gccaaccggc gggctggccg cctgccctct ctcgtgctgg 121 tggtgctgct ggtggtgatc gtcgtcctcg ccttcaacta ctggagcatc tcctcccgcc 181 acgtcctgct tcaggaggag gtggccgagc tgcagggcca ggtccagcgc accgaagtgg 241 cccgcgggcg gctggaaaag cgcaattcgg acctcttgct gttggtggac acgcacaaga 301 aacagatcga ccagaaggag gccgactacg gccgcctcag cagccggctg caggccagag 361 agggcctcgg gaagagatgc gaggatgaca aggttaaact acagaacaac atatcgtatc 421 agatggcaga catacatcat ttaaaggagc aacttgctga gcttcgtcag gaatttcttc 481 gacaagaaga ccagcttcag gactatagga agaacaatac ttaccttgtg aagaggttag 541 aatatgaaag caagagaccc aaaagattca atcaaatgat ggaaaggaat tggatatacc 601 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 661 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 721 tta