Construct: ORF ccsbBroad304_13083
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003456.1_s304c1, BRDN0000461370
- Derived from:
- ccsbBroadEn_13083
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGD4 (121512)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13083
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_001304483.1 | 99.7% | 99.7% | (many diffs) |
2 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_005253310.4 | 87.3% | 84.2% | (many diffs) |
3 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_011520559.3 | 83.5% | 78.7% | (many diffs) |
4 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_011520558.2 | 79.8% | 77% | (many diffs) |
5 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_024448840.1 | 78% | 74.8% | (many diffs) |
6 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_001370297.1 | 77.8% | 75% | (many diffs) |
7 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_011520557.1 | 77.8% | 75% | (many diffs) |
8 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_001304484.1 | 69% | 65.8% | (many diffs) |
9 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_001330373.1 | 67.4% | 64.9% | (many diffs) |
10 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_001330374.1 | 67.4% | 64.9% | (many diffs) |
11 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_005253308.5 | 67.4% | 64.9% | (many diffs) |
12 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_005253309.1 | 67.4% | 64.9% | (many diffs) |
13 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_024448837.1 | 67.4% | 64.9% | (many diffs) |
14 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_024448838.1 | 67.4% | 64.9% | (many diffs) |
15 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_024448839.1 | 67.4% | 64.9% | (many diffs) |
16 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_139241.3 | 59.2% | 57% | (many diffs) |
17 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_011520555.1 | 59.2% | 57% | (many diffs) |
18 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_011520556.1 | 59.2% | 57% | (many diffs) |
19 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_001304481.1 | 53.3% | 51.4% | (many diffs) |
20 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_011520554.1 | 52.5% | 50.6% | (many diffs) |
21 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_001304480.1 | 51.7% | 49.8% | (many diffs) |
22 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XM_017018803.1 | 49.7% | 47.8% | (many diffs) |
23 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | NM_001370298.1 | 48.8% | 47% | (many diffs) |
24 | human | 121512 | FGD4 | FYVE, RhoGEF and PH domain ... | XR_001748576.1 | 20.6% | (many diffs) | |
25 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | XM_011245874.2 | 55.9% | 57.5% | (many diffs) |
26 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | NM_001301818.1 | 52.7% | 54.2% | (many diffs) |
27 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | NM_139232.3 | 52.7% | 54.2% | (many diffs) |
28 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | XM_006522027.3 | 52.7% | 54.2% | (many diffs) |
29 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | XM_006522029.2 | 52.7% | 54.2% | (many diffs) |
30 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | XM_006522025.3 | 51.6% | 53% | (many diffs) |
31 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | XM_006522024.3 | 49.9% | 51.2% | (many diffs) |
32 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | XM_006522023.2 | 48.1% | 49.4% | (many diffs) |
33 | mouse | 224014 | Fgd4 | FYVE, RhoGEF and PH domain ... | NM_001301817.1 | 45.6% | 46.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1482
- ORF length:
- 1413
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgaataaa atcttttcta atatttcatc aataaatgcc ttccatagta 121 aattcctctt gccagagctg gagaaacgaa tgcaagaatg ggaaactact cctagaattg 181 gagacatcct tcagaaattg gcaccattcc ttaagatgta tggagaatat gtgaaaggat 241 ttgataatgc aatggaattg gttaaaaaca tgacagaacg tattccccag ttcaaatcag 301 tggttgaaga aattcagaaa cagaaaatct gtgggagctt aactttgcag catcacatgc 361 tagaacctgt tcagcggatt ccccggtatg agatgctcct taaggactat ctaaggaaat 421 tgcctcctga ttccctggac tggaatgatg ctaaaaaatc acttgaaatt atatctacag 481 ctgcaagcca ttctaatagt gcaataagga aaatggagaa cctaaagaaa ctcttagaga 541 tttatgaaat gttgggagaa gaagaagaca ttgtaaaccc ttcaaatgaa ctaataaaag 601 aaggacagat cctcaaacta gctgctcgaa acacttcagc acgagaacgc taccttttct 661 tattcaacaa catgttgctg tactgtgtgc ccaaattcag cttggtaggc tctaaattca 721 cagttcgaac cagggttggc attgatggaa tgaaaattgt agagactcaa aatgaagaat 781 atccacatac tttccaggtg tctgggaaag agagaacact ggaactgcag gccagttctg 841 cacaagacaa agaagaatgg atcaaggccc ttcaagaaac catcgatgct tttcatcaaa 901 ggcatgaaac cttcagaaat gcaattgcaa aggataatga cattcactca gaggtttcta 961 ctgctgagct agggaaaaga gccccaagat ggatccgaga taatgaagtg acaatgtgta 1021 tgaaatgtaa agaacctttc aatgcactga cacgaaggag gcatcattgt cgagcatgtg 1081 gatatgtggt ttgttggaaa tgctccgact acaaagctca acttgaatat gatggtggta 1141 aattgagcaa agtttgtaaa gactgttatc aaatcataag tggattcaca gacagtgaag 1201 aaaagaaaag aaaaggaatt ttagagattg aatcagcaga agtatctgga aacagtgtgg 1261 tgtgcagctt tcttcagtat atggagaagt caaaaccttg gcagaaagct tggtgtgtga 1321 tccccaagca agaccctctt gtgctgtaca tgtatggtgc cccccaggta tctaaaccac 1381 atctgtctga agggacagat gcccttgggg gcaaggggaa gcgagtggac agcggactca 1441 aaatctgtag aacaagagtt caggcagttt cactgtttca tttgccaact ttcttgtaca 1501 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1561 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta