Transcript: Human XM_005253309.1

PREDICTED: Homo sapiens FYVE, RhoGEF and PH domain containing 4 (FGD4), transcript variant X10, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FGD4 (121512)
Length:
7983
CDS:
445..2466

Additional Resources:

NCBI RefSeq record:
XM_005253309.1
NBCI Gene record:
FGD4 (121512)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005253309.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048236 CCCTTGCTTGATACGCACATA pLKO.1 526 CDS 100% 4.950 6.930 N FGD4 n/a
2 TRCN0000048235 CCGAGATAATGAAGTGACAAT pLKO.1 1836 CDS 100% 4.950 6.930 N FGD4 n/a
3 TRCN0000416144 TTGTGTGGCCAACGGTGTAAT pLKO_005 426 5UTR 100% 13.200 10.560 N FGD4 n/a
4 TRCN0000048234 GCCATTCTAATAGTGCAATAA pLKO.1 1328 CDS 100% 13.200 9.240 N FGD4 n/a
5 TRCN0000446100 GACACGAAGGAGGCATCATTG pLKO_005 1890 CDS 100% 10.800 7.560 N FGD4 n/a
6 TRCN0000048237 CATCAATAAATGCCTTCCATA pLKO.1 938 CDS 100% 4.950 3.465 N FGD4 n/a
7 TRCN0000048233 CCATGAGATGAAGGAGACTAA pLKO.1 753 CDS 100% 4.950 3.465 N FGD4 n/a
8 TRCN0000425877 AGAATGAGCACTTCCATTTAA pLKO_005 2764 3UTR 100% 15.000 9.000 N FGD4 n/a
9 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 7390 3UTR 100% 4.950 2.475 Y ORAI2 n/a
10 TRCN0000109956 CCTTCCATAGTAAATTCCTAT pLKO.1 950 CDS 100% 4.950 3.960 N Fgd4 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 7464 3UTR 100% 13.200 6.600 Y LIAS n/a
12 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 7597 3UTR 100% 10.800 5.400 Y SMIM11A n/a
13 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 7387 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005253309.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13083 pDONR223 100% 67.4% 64.9% None (many diffs) n/a
2 ccsbBroad304_13083 pLX_304 0% 67.4% 64.9% V5 (many diffs) n/a
3 TRCN0000491703 AGACAAAGGTGCAGGATCGAAAAT pLX_317 24.1% 67.4% 64.9% V5 (many diffs) n/a
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