Transcript: Mouse XM_006522029.2

PREDICTED: Mus musculus FYVE, RhoGEF and PH domain containing 4 (Fgd4), transcript variant X6, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fgd4 (224014)
Length:
8189
CDS:
236..2536

Additional Resources:

NCBI RefSeq record:
XM_006522029.2
NBCI Gene record:
Fgd4 (224014)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006522029.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000433601 GAATCACTACCTGGTTATAAT pLKO_005 2814 3UTR 100% 15.000 21.000 N Fgd4 n/a
2 TRCN0000109957 GCAGAAAGATTCTACTATGAA pLKO.1 352 CDS 100% 5.625 7.875 N Fgd4 n/a
3 TRCN0000109958 GCCCTTCAAGAAAGTATTGAT pLKO.1 1778 CDS 100% 5.625 7.875 N Fgd4 n/a
4 TRCN0000048237 CATCAATAAATGCCTTCCATA pLKO.1 1008 CDS 100% 4.950 6.930 N FGD4 n/a
5 TRCN0000109956 CCTTCCATAGTAAATTCCTAT pLKO.1 1020 CDS 100% 4.950 3.960 N Fgd4 n/a
6 TRCN0000048234 GCCATTCTAATAGTGCAATAA pLKO.1 1398 CDS 100% 13.200 9.240 N FGD4 n/a
7 TRCN0000424687 TACGTGAAGGGATTTGATAAT pLKO_005 1139 CDS 100% 13.200 9.240 N Fgd4 n/a
8 TRCN0000109955 CCAGTAATACACATAGCTCAA pLKO.1 2564 3UTR 100% 4.050 2.835 N Fgd4 n/a
9 TRCN0000109959 CCAGTTTAAATCAGTGACTGA pLKO.1 1198 CDS 100% 2.640 1.848 N Fgd4 n/a
10 TRCN0000423513 ATGAAGTGAAGGAGACTAATG pLKO_005 825 CDS 100% 10.800 6.480 N Fgd4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006522029.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13083 pDONR223 100% 52.7% 54.2% None (many diffs) n/a
2 ccsbBroad304_13083 pLX_304 0% 52.7% 54.2% V5 (many diffs) n/a
3 TRCN0000491703 AGACAAAGGTGCAGGATCGAAAAT pLX_317 24.1% 52.7% 54.2% V5 (many diffs) n/a
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